Identification of four genomic loci highly related to casein-kinase-2-alpha cDNA and characterization of a casein kinase-2-alpha pseudogene within the mouse genome

B Boldyreff, K Wehr, R Hecht, O G Issinger

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1992-Jul-31
OriginalsprogEngelsk
TidsskriftBiochemical and Biophysical Research Communications
Vol/bind186
Udgave nummer2
Sider (fra-til)723-730
Antal sider7
ISSN0006-291X
StatusUdgivet - 31. jul. 1992

Fingeraftryk

Casein Kinase II
Pseudogenes
Nucleotides
Complementary DNA
Clone Cells
Genes
DNA sequences
Introns
Screening
Genomic Library

Citer dette

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title = "Identification of four genomic loci highly related to casein-kinase-2-alpha cDNA and characterization of a casein kinase-2-alpha pseudogene within the mouse genome",
abstract = "Using the coding region of the human CK-2 alpha cDNA as a probe for screening a genomic mouse library, positive clones representing four different genomic loci were isolated. Partial DNA sequences of these loci encompassing the first 120 nucleotides of the putative coding region are reported. One positive clone was further analyzed by sequencing a 3.1 kb XbaI fragment. This clone displays the characteristics of a pseudogene, i.e. lack of introns and several nucleotide insertions and deletions. In its 3' region it contains a 91 bp large CT-rich stretch which consists of (CCTT) and (CT) repeats; in the 5' region three (CCCCCT) repeats.",
keywords = "Animals, Base Sequence, Blotting, Southern, Casein Kinases, DNA, Gene Library, Genome, Humans, Mice, Molecular Sequence Data, Protein Kinases, Pseudogenes, Repetitive Sequences, Nucleic Acid, Restriction Mapping, Sequence Homology, Nucleic Acid",
author = "B Boldyreff and K Wehr and R Hecht and Issinger, {O G}",
year = "1992",
month = "7",
day = "31",
language = "English",
volume = "186",
pages = "723--730",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
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Identification of four genomic loci highly related to casein-kinase-2-alpha cDNA and characterization of a casein kinase-2-alpha pseudogene within the mouse genome. / Boldyreff, B; Wehr, K; Hecht, R; Issinger, O G.

I: Biochemical and Biophysical Research Communications, Bind 186, Nr. 2, 31.07.1992, s. 723-730.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Identification of four genomic loci highly related to casein-kinase-2-alpha cDNA and characterization of a casein kinase-2-alpha pseudogene within the mouse genome

AU - Boldyreff, B

AU - Wehr, K

AU - Hecht, R

AU - Issinger, O G

PY - 1992/7/31

Y1 - 1992/7/31

N2 - Using the coding region of the human CK-2 alpha cDNA as a probe for screening a genomic mouse library, positive clones representing four different genomic loci were isolated. Partial DNA sequences of these loci encompassing the first 120 nucleotides of the putative coding region are reported. One positive clone was further analyzed by sequencing a 3.1 kb XbaI fragment. This clone displays the characteristics of a pseudogene, i.e. lack of introns and several nucleotide insertions and deletions. In its 3' region it contains a 91 bp large CT-rich stretch which consists of (CCTT) and (CT) repeats; in the 5' region three (CCCCCT) repeats.

AB - Using the coding region of the human CK-2 alpha cDNA as a probe for screening a genomic mouse library, positive clones representing four different genomic loci were isolated. Partial DNA sequences of these loci encompassing the first 120 nucleotides of the putative coding region are reported. One positive clone was further analyzed by sequencing a 3.1 kb XbaI fragment. This clone displays the characteristics of a pseudogene, i.e. lack of introns and several nucleotide insertions and deletions. In its 3' region it contains a 91 bp large CT-rich stretch which consists of (CCTT) and (CT) repeats; in the 5' region three (CCCCCT) repeats.

KW - Animals

KW - Base Sequence

KW - Blotting, Southern

KW - Casein Kinases

KW - DNA

KW - Gene Library

KW - Genome

KW - Humans

KW - Mice

KW - Molecular Sequence Data

KW - Protein Kinases

KW - Pseudogenes

KW - Repetitive Sequences, Nucleic Acid

KW - Restriction Mapping

KW - Sequence Homology, Nucleic Acid

M3 - Journal article

VL - 186

SP - 723

EP - 730

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -