Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha

Silvia E. Glont, Evangelia K. Papachristou, Ashley Sawle, Kelly A. Holmes, Jason S. Carroll*, Rasmus Siersbaek

*Kontaktforfatter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

71 Downloads (Pure)

Abstract

Estrogen Receptor alpha (ERα) plays a major role in most breast cancers, and it is the target of endocrine therapies used in the clinic as standard of care for women with breast cancer expressing this receptor. The two methods ChIP-seq (chromatin immunoprecipitation coupled with deep sequencing) and RIME (Rapid Immunoprecipitation of Endogenous Proteins) have greatly improved our understanding of ERα function during breast cancer progression and in response to anti-estrogens. A critical component of both ChIP-seq and RIME protocols is the antibody that is used against the bait protein. To date, most of the ChIP-seq and RIME experiments for the study of ERα have been performed using the sc-543 antibody from Santa Cruz Biotechnology. However, this antibody has been discontinued, thereby severely impacting the study of ERα in normal physiology as well as diseases such as breast cancer and ovarian cancer. Here, we compare the sc-543 antibody with other commercially available antibodies, and we show that 06–935 (EMD Millipore) and ab3575 (Abcam) antibodies can successfully replace the sc-543 antibody for ChIP-seq and RIME experiments.

OriginalsprogEngelsk
Artikelnummere0215340
TidsskriftPLOS ONE
Vol/bind14
Udgave nummer4
Antal sider10
ISSN1932-6203
DOI
StatusUdgivet - 10. apr. 2019
Udgivet eksterntJa

Fingeraftryk

Dyk ned i forskningsemnerne om 'Identification of ChIP-seq and RIME grade antibodies for Estrogen Receptor alpha'. Sammen danner de et unikt fingeraftryk.

Citationsformater