Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Toke Peter Krogager; Lone Vendel Nielsen; Steffen Bak; Clifford Young; Carla Ferreri; Ole Nørregaard Jensen; Peter Højrup; Vladimiros Thoma; Ida Thøgersen; Jan J. Enghild

doi:10.1016/j.jprot.2012.03.023

Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Toke Peter Krogager, Lone Vendel Nielsen, Steffen Bak, Clifford Young, Carla Ferreri, Ole Nørregaard Jensen, Peter Højrup, Vladimiros Thoma, Ida Thøgersen, Jan J. Enghild

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

Abstrakt

Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value

Originalsprog	Engelsk
Tidsskrift	Journal of Proteomics
Vol/bind	75
Udgave nummer	9
Sider (fra-til)	2685-96
Antal sider	12
ISSN	1874-3919
DOI	https://doi.org/10.1016/j.jprot.2012.03.023
Status	Udgivet - 2012

Dokumenter og links

10.1016/j.jprot.2012.03.023

Citationsformater

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title = "Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)",

abstract = "Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value",

author = "Krogager, {Toke Peter} and Nielsen, {Lone Vendel} and Steffen Bak and Clifford Young and Carla Ferreri and Jensen, {Ole N{\o}rregaard} and Peter H{\o}jrup and Vladimiros Thoma and Ida Th{\o}gersen and Enghild, {Jan J.}",

year = "2012",

doi = "10.1016/j.jprot.2012.03.023",

language = "English",

volume = "75",

pages = "2685--96",

journal = "Journal of Proteomics",

issn = "1874-3919",

publisher = "Elsevier",

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TY - JOUR

T1 - Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

AU - Krogager, Toke Peter

AU - Nielsen, Lone Vendel

AU - Bak, Steffen

AU - Young, Clifford

AU - Ferreri, Carla

AU - Jensen, Ole Nørregaard

AU - Højrup, Peter

AU - Thoma, Vladimiros

AU - Thøgersen, Ida

AU - Enghild, Jan J.

PY - 2012

Y1 - 2012

N2 - Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value

AB - Trans fatty acid intake has been correlated to an unfavorable plasma lipoprotein profile and an increased cardiovascular disease risk. The present study aimed to identify a plasma protein biomarker panel related to human intake of elaidic acid. The human liver cell line HepG2-SF was used as a model system, and the cells were maintained for seven days in serum-free medium containing 100 μM elaidic acid (trans∆9-C18:1), oleic acid (cis∆9-C18:1) or stearic acid (C18:0). The secretomes were analyzed by stable isotope labeling of amino acids in cell culture (SILAC), difference in gel electrophoresis (DIGE) and gene expression microarray analysis. Twelve proteins were found to be differentially regulated based on SILAC data (>1.3 fold change, P-value1.3 fold change, P-value1.3 fold change, P-value

U2 - 10.1016/j.jprot.2012.03.023

DO - 10.1016/j.jprot.2012.03.023

M3 - Journal article

C2 - 22483997

VL - 75

SP - 2685

EP - 2696

JO - Journal of Proteomics

JF - Journal of Proteomics

SN - 1874-3919

IS - 9

ER -

Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans∆9-C18:1)

Abstrakt

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