Hydrophobic mismatch triggering texture defects in membrane gel domains

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The orientational texture of gel-phase lipid bilayers is a phenomenon that can structure membrane domains. Using two-photon polarized fluorescence microscopy and image analysis, we map the lateral variation of the lipid orientation (the texture) in single domains. With this method, we uncover a lipid-induced transition between vortex and uniform textures in binary phospholipid bilayers. By tuning the lipid composition, the hydrophobic mismatch at the domain boundary can be varied systematically as monitored by AFM. Low hydrophobic mismatch correlates with domains having uniform texture, while higher mismatch values correlate with a vortex-type texture. The defect pattern created during early growth persists in larger domains, and a minimal model incorporating the anisotropic line tension and the vortex energy can rationalize this finding. The results suggest that the lipid composition and the domain nucleation process are critical factors that determine the texture pattern of membrane domains.
OriginalsprogEngelsk
TidsskriftThe Journal of Physical Chemistry Letters
Vol/bind4
Udgave nummer17
Sider (fra-til)2789-2793
ISSN1948-7185
DOI
StatusUdgivet - 2013

Fingeraftryk

Gels
Textures
Membranes
Defects
Lipids
Vortex flow
Membrane structures
Lipid bilayers
Fluorescence microscopy
Phospholipids
Chemical analysis
Image analysis
Nucleation
Photons
Tuning

Citer dette

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abstract = "The orientational texture of gel-phase lipid bilayers is a phenomenon that can structure membrane domains. Using two-photon polarized fluorescence microscopy and image analysis, we map the lateral variation of the lipid orientation (the texture) in single domains. With this method, we uncover a lipid-induced transition between vortex and uniform textures in binary phospholipid bilayers. By tuning the lipid composition, the hydrophobic mismatch at the domain boundary can be varied systematically as monitored by AFM. Low hydrophobic mismatch correlates with domains having uniform texture, while higher mismatch values correlate with a vortex-type texture. The defect pattern created during early growth persists in larger domains, and a minimal model incorporating the anisotropic line tension and the vortex energy can rationalize this finding. The results suggest that the lipid composition and the domain nucleation process are critical factors that determine the texture pattern of membrane domains.",
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Hydrophobic mismatch triggering texture defects in membrane gel domains. / Dreier, J.; Brewer, J.R.; Simonsen, Adam Cohen.

I: The Journal of Physical Chemistry Letters, Bind 4, Nr. 17, 2013, s. 2789-2793.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Hydrophobic mismatch triggering texture defects in membrane gel domains

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AU - Brewer, J.R.

AU - Simonsen, Adam Cohen

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