Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340

T J Ligtenberg, F J Bikker, J Groenink, I Tornoe, Rikke Leth-Larsen, E C Veerman, A V Nieuw Amerongen, U Holmskov

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Salivary agglutinin is a 300-400 kDa salivary glycoprotein that binds to antigen B polypeptides of oral streptococci, thereby playing a role in their colonization and the development of caries. A mass spectrum was recorded of a trypsin digest of agglutinin. A dominant peak of 1460 Da was sequenced by quadrupole time-of-flight (Q-TOF) tandem MS. The sequence showed 100% identity with part of the scavenger receptor cysteine-rich ('SRCR') domain found in gp-340/DMBT1 (deleted in malignant brain tumours-1). The mass spectrum revealed 11 peaks with an identical mass as a computer-simulated trypsin digest of gp-340. gp-340 is a 340 kDa glycoprotein isolated from bronchoalveolar lavage fluid that binds specifically to lung surfactant protein-D. DMBT1 is a candidate tumour suppressor gene. A search in the human genome revealed only one copy of this gene. The molecular mass, as judged from SDS/PAGE and the amino acid composition of agglutinin, was found to be nearly identical with that of gp-340. It was shown by Western blotting that monoclonal antibodies against gp-340 reacted with salivary agglutinin, and monoclonals against agglutinin reacted with gp-340. It was demonstrated that gp-340 and agglutinin bound in a similar way to Streptococcus mutans and surfactant protein-D. Histochemically, the distribution of gp-340 in the submandibular salivary glands was identical with the agglutinin distribution, as shown in a previous paper [Takano, Bogert, Malamud, Lally and Hand (1991) Anat. Rec. 230, 307-318]. We conclude that agglutinin is identical with gp-340, and that this molecule interacts with S. mutans and surfactant protein-D.

OriginalsprogEngelsk
TidsskriftThe Biochemical journal
Vol/bind359
Udgave nummerPt 1
Sider (fra-til)243-8
Antal sider6
ISSN0264-6021
StatusUdgivet - 1. okt. 2001

Fingeraftryk

Pulmonary Surfactant-Associated Proteins
Pulmonary Surfactant-Associated Protein D
Scavenger Receptors
Agglutinins
Proteins
Genes
Trypsin
Tumors
Glycoproteins
glycoprotein 340
Molecular mass
Human Genome
Cysteine
Brain
Monoclonal Antibodies

Citer dette

Ligtenberg, T J ; Bikker, F J ; Groenink, J ; Tornoe, I ; Leth-Larsen, Rikke ; Veerman, E C ; Nieuw Amerongen, A V ; Holmskov, U. / Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340. I: The Biochemical journal. 2001 ; Bind 359, Nr. Pt 1. s. 243-8.
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title = "Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340",
abstract = "Salivary agglutinin is a 300-400 kDa salivary glycoprotein that binds to antigen B polypeptides of oral streptococci, thereby playing a role in their colonization and the development of caries. A mass spectrum was recorded of a trypsin digest of agglutinin. A dominant peak of 1460 Da was sequenced by quadrupole time-of-flight (Q-TOF) tandem MS. The sequence showed 100{\%} identity with part of the scavenger receptor cysteine-rich ('SRCR') domain found in gp-340/DMBT1 (deleted in malignant brain tumours-1). The mass spectrum revealed 11 peaks with an identical mass as a computer-simulated trypsin digest of gp-340. gp-340 is a 340 kDa glycoprotein isolated from bronchoalveolar lavage fluid that binds specifically to lung surfactant protein-D. DMBT1 is a candidate tumour suppressor gene. A search in the human genome revealed only one copy of this gene. The molecular mass, as judged from SDS/PAGE and the amino acid composition of agglutinin, was found to be nearly identical with that of gp-340. It was shown by Western blotting that monoclonal antibodies against gp-340 reacted with salivary agglutinin, and monoclonals against agglutinin reacted with gp-340. It was demonstrated that gp-340 and agglutinin bound in a similar way to Streptococcus mutans and surfactant protein-D. Histochemically, the distribution of gp-340 in the submandibular salivary glands was identical with the agglutinin distribution, as shown in a previous paper [Takano, Bogert, Malamud, Lally and Hand (1991) Anat. Rec. 230, 307-318]. We conclude that agglutinin is identical with gp-340, and that this molecule interacts with S. mutans and surfactant protein-D.",
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Ligtenberg, TJ, Bikker, FJ, Groenink, J, Tornoe, I, Leth-Larsen, R, Veerman, EC, Nieuw Amerongen, AV & Holmskov, U 2001, 'Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340', The Biochemical journal, bind 359, nr. Pt 1, s. 243-8.

Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340. / Ligtenberg, T J; Bikker, F J; Groenink, J; Tornoe, I; Leth-Larsen, Rikke; Veerman, E C; Nieuw Amerongen, A V; Holmskov, U.

I: The Biochemical journal, Bind 359, Nr. Pt 1, 01.10.2001, s. 243-8.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Human salivary agglutinin binds to lung surfactant protein-D and is identical with scavenger receptor protein gp-340

AU - Ligtenberg, T J

AU - Bikker, F J

AU - Groenink, J

AU - Tornoe, I

AU - Leth-Larsen, Rikke

AU - Veerman, E C

AU - Nieuw Amerongen, A V

AU - Holmskov, U

PY - 2001/10/1

Y1 - 2001/10/1

N2 - Salivary agglutinin is a 300-400 kDa salivary glycoprotein that binds to antigen B polypeptides of oral streptococci, thereby playing a role in their colonization and the development of caries. A mass spectrum was recorded of a trypsin digest of agglutinin. A dominant peak of 1460 Da was sequenced by quadrupole time-of-flight (Q-TOF) tandem MS. The sequence showed 100% identity with part of the scavenger receptor cysteine-rich ('SRCR') domain found in gp-340/DMBT1 (deleted in malignant brain tumours-1). The mass spectrum revealed 11 peaks with an identical mass as a computer-simulated trypsin digest of gp-340. gp-340 is a 340 kDa glycoprotein isolated from bronchoalveolar lavage fluid that binds specifically to lung surfactant protein-D. DMBT1 is a candidate tumour suppressor gene. A search in the human genome revealed only one copy of this gene. The molecular mass, as judged from SDS/PAGE and the amino acid composition of agglutinin, was found to be nearly identical with that of gp-340. It was shown by Western blotting that monoclonal antibodies against gp-340 reacted with salivary agglutinin, and monoclonals against agglutinin reacted with gp-340. It was demonstrated that gp-340 and agglutinin bound in a similar way to Streptococcus mutans and surfactant protein-D. Histochemically, the distribution of gp-340 in the submandibular salivary glands was identical with the agglutinin distribution, as shown in a previous paper [Takano, Bogert, Malamud, Lally and Hand (1991) Anat. Rec. 230, 307-318]. We conclude that agglutinin is identical with gp-340, and that this molecule interacts with S. mutans and surfactant protein-D.

AB - Salivary agglutinin is a 300-400 kDa salivary glycoprotein that binds to antigen B polypeptides of oral streptococci, thereby playing a role in their colonization and the development of caries. A mass spectrum was recorded of a trypsin digest of agglutinin. A dominant peak of 1460 Da was sequenced by quadrupole time-of-flight (Q-TOF) tandem MS. The sequence showed 100% identity with part of the scavenger receptor cysteine-rich ('SRCR') domain found in gp-340/DMBT1 (deleted in malignant brain tumours-1). The mass spectrum revealed 11 peaks with an identical mass as a computer-simulated trypsin digest of gp-340. gp-340 is a 340 kDa glycoprotein isolated from bronchoalveolar lavage fluid that binds specifically to lung surfactant protein-D. DMBT1 is a candidate tumour suppressor gene. A search in the human genome revealed only one copy of this gene. The molecular mass, as judged from SDS/PAGE and the amino acid composition of agglutinin, was found to be nearly identical with that of gp-340. It was shown by Western blotting that monoclonal antibodies against gp-340 reacted with salivary agglutinin, and monoclonals against agglutinin reacted with gp-340. It was demonstrated that gp-340 and agglutinin bound in a similar way to Streptococcus mutans and surfactant protein-D. Histochemically, the distribution of gp-340 in the submandibular salivary glands was identical with the agglutinin distribution, as shown in a previous paper [Takano, Bogert, Malamud, Lally and Hand (1991) Anat. Rec. 230, 307-318]. We conclude that agglutinin is identical with gp-340, and that this molecule interacts with S. mutans and surfactant protein-D.

KW - Agglutinins

KW - Antibodies, Monoclonal

KW - Blotting, Western

KW - Brain Neoplasms

KW - Genome, Human

KW - Humans

KW - Lung

KW - Membrane Proteins

KW - Parotid Gland

KW - Protein Binding

KW - Pulmonary Surfactant-Associated Protein D

KW - Pulmonary Surfactants

KW - Receptors, Cell Surface

KW - Receptors, Immunologic

KW - Receptors, Lipoprotein

KW - Receptors, Scavenger

KW - Saliva

KW - Scavenger Receptors, Class B

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Streptococcus mutans

M3 - Journal article

C2 - 11563989

VL - 359

SP - 243

EP - 248

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - Pt 1

ER -