Resumé
BACKGROUND:: Natural killer (NK) cell phenotype and function have recently gained much attention as playing crucial roles in antibody-dependent cellular cytotoxicity (ADCC). We investigated NK cell function, as measured by ADCC, in HIV-1 positive individuals before and six months after highly active antiretroviral therapy (HAART) initiation.
METHOD:: The ability of antibodies and NK cells to mediate ADCC was investigated separately and in combination in an autologous model. The NK cell subset distribution and NK cell phenotype (i.e. expression of maturation and activation markers within NK cell subsets) were analyzed.
RESULTS:: The ability of NK cells to mediate ADCC was significantly increased after only six months of HAART and was not explained by a normalization of NK cell subsets (CD56CD16 and CD56CD16 NK cells) but rather by normalization in the frequency of NK cells expressing CCR7 and CD27. For individuals with no increase in ADCC after six months of HAART, the frequency of NK cells expressing NKp46 was down-regulated. The ability of antibodies to mediate ADCC alone and in combination in an autologous model was not improved.
CONCLUSION:: HAART improves the ability of NK cells to mediate ADCC after six months. This improvement does not correlate with general immune restoration, as measured by CD4 T cell counts, but rather to a decrease in the frequency of NK cells expressing CCR7 and CD27.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | J A I D S |
| Vol/bind | 68 |
| Udgave nummer | 2 |
| Sider (fra-til) | 103-111 |
| ISSN | 1525-4135 |
| DOI | |
| Status | Udgivet - 2015 |
Fingeraftryk
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HIV-specific ADCC improves after antiretroviral therapy and correlates with normalization of the NK cell phenotype. / Jensen, Sanne Skov; Hartling, Hans Jakob; Tingstedt, Jeanette Linnea; Larsen, Tine ; Nielsen, Susanne Dam; Pedersen, Court; Fomsgaard, Anders; Karlsson, Ingrid.
I: J A I D S, Bind 68, Nr. 2, 2015, s. 103-111.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
TY - JOUR
T1 - HIV-specific ADCC improves after antiretroviral therapy and correlates with normalization of the NK cell phenotype
AU - Jensen, Sanne Skov
AU - Hartling, Hans Jakob
AU - Tingstedt, Jeanette Linnea
AU - Larsen, Tine
AU - Nielsen, Susanne Dam
AU - Pedersen, Court
AU - Fomsgaard, Anders
AU - Karlsson, Ingrid
PY - 2015
Y1 - 2015
N2 - BACKGROUND:: Natural killer (NK) cell phenotype and function have recently gained much attention as playing crucial roles in antibody-dependent cellular cytotoxicity (ADCC). We investigated NK cell function, as measured by ADCC, in HIV-1 positive individuals before and six months after highly active antiretroviral therapy (HAART) initiation.METHOD:: The ability of antibodies and NK cells to mediate ADCC was investigated separately and in combination in an autologous model. The NK cell subset distribution and NK cell phenotype (i.e. expression of maturation and activation markers within NK cell subsets) were analyzed.RESULTS:: The ability of NK cells to mediate ADCC was significantly increased after only six months of HAART and was not explained by a normalization of NK cell subsets (CD56CD16 and CD56CD16 NK cells) but rather by normalization in the frequency of NK cells expressing CCR7 and CD27. For individuals with no increase in ADCC after six months of HAART, the frequency of NK cells expressing NKp46 was down-regulated. The ability of antibodies to mediate ADCC alone and in combination in an autologous model was not improved.CONCLUSION:: HAART improves the ability of NK cells to mediate ADCC after six months. This improvement does not correlate with general immune restoration, as measured by CD4 T cell counts, but rather to a decrease in the frequency of NK cells expressing CCR7 and CD27.
AB - BACKGROUND:: Natural killer (NK) cell phenotype and function have recently gained much attention as playing crucial roles in antibody-dependent cellular cytotoxicity (ADCC). We investigated NK cell function, as measured by ADCC, in HIV-1 positive individuals before and six months after highly active antiretroviral therapy (HAART) initiation.METHOD:: The ability of antibodies and NK cells to mediate ADCC was investigated separately and in combination in an autologous model. The NK cell subset distribution and NK cell phenotype (i.e. expression of maturation and activation markers within NK cell subsets) were analyzed.RESULTS:: The ability of NK cells to mediate ADCC was significantly increased after only six months of HAART and was not explained by a normalization of NK cell subsets (CD56CD16 and CD56CD16 NK cells) but rather by normalization in the frequency of NK cells expressing CCR7 and CD27. For individuals with no increase in ADCC after six months of HAART, the frequency of NK cells expressing NKp46 was down-regulated. The ability of antibodies to mediate ADCC alone and in combination in an autologous model was not improved.CONCLUSION:: HAART improves the ability of NK cells to mediate ADCC after six months. This improvement does not correlate with general immune restoration, as measured by CD4 T cell counts, but rather to a decrease in the frequency of NK cells expressing CCR7 and CD27.
U2 - 10.1097/QAI.0000000000000429
DO - 10.1097/QAI.0000000000000429
M3 - Journal article
C2 - 25394194
VL - 68
SP - 103
EP - 111
JO - J A I D S
JF - J A I D S
SN - 1525-4135
IS - 2
ER -