HIV-specific ADCC improves after antiretroviral therapy and correlates with normalization of the NK cell phenotype

Sanne Skov Jensen, Hans Jakob Hartling, Jeanette Linnea Tingstedt, Tine Larsen, Susanne Dam Nielsen, Court Pedersen, Anders Fomsgaard, Ingrid Karlsson

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

BACKGROUND:: Natural killer (NK) cell phenotype and function have recently gained much attention as playing crucial roles in antibody-dependent cellular cytotoxicity (ADCC). We investigated NK cell function, as measured by ADCC, in HIV-1 positive individuals before and six months after highly active antiretroviral therapy (HAART) initiation.

METHOD:: The ability of antibodies and NK cells to mediate ADCC was investigated separately and in combination in an autologous model. The NK cell subset distribution and NK cell phenotype (i.e. expression of maturation and activation markers within NK cell subsets) were analyzed.

RESULTS:: The ability of NK cells to mediate ADCC was significantly increased after only six months of HAART and was not explained by a normalization of NK cell subsets (CD56CD16 and CD56CD16 NK cells) but rather by normalization in the frequency of NK cells expressing CCR7 and CD27. For individuals with no increase in ADCC after six months of HAART, the frequency of NK cells expressing NKp46 was down-regulated. The ability of antibodies to mediate ADCC alone and in combination in an autologous model was not improved.

CONCLUSION:: HAART improves the ability of NK cells to mediate ADCC after six months. This improvement does not correlate with general immune restoration, as measured by CD4 T cell counts, but rather to a decrease in the frequency of NK cells expressing CCR7 and CD27.

OriginalsprogEngelsk
TidsskriftJ A I D S
Vol/bind68
Udgave nummer2
Sider (fra-til)103-111
ISSN1525-4135
DOI
StatusUdgivet - 2015

Fingeraftryk

HIV Antibodies
HIV-1

Citer dette

Jensen, Sanne Skov ; Hartling, Hans Jakob ; Tingstedt, Jeanette Linnea ; Larsen, Tine ; Nielsen, Susanne Dam ; Pedersen, Court ; Fomsgaard, Anders ; Karlsson, Ingrid. / HIV-specific ADCC improves after antiretroviral therapy and correlates with normalization of the NK cell phenotype. I: J A I D S. 2015 ; Bind 68, Nr. 2. s. 103-111.
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title = "HIV-specific ADCC improves after antiretroviral therapy and correlates with normalization of the NK cell phenotype",
abstract = "BACKGROUND:: Natural killer (NK) cell phenotype and function have recently gained much attention as playing crucial roles in antibody-dependent cellular cytotoxicity (ADCC). We investigated NK cell function, as measured by ADCC, in HIV-1 positive individuals before and six months after highly active antiretroviral therapy (HAART) initiation.METHOD:: The ability of antibodies and NK cells to mediate ADCC was investigated separately and in combination in an autologous model. The NK cell subset distribution and NK cell phenotype (i.e. expression of maturation and activation markers within NK cell subsets) were analyzed.RESULTS:: The ability of NK cells to mediate ADCC was significantly increased after only six months of HAART and was not explained by a normalization of NK cell subsets (CD56CD16 and CD56CD16 NK cells) but rather by normalization in the frequency of NK cells expressing CCR7 and CD27. For individuals with no increase in ADCC after six months of HAART, the frequency of NK cells expressing NKp46 was down-regulated. The ability of antibodies to mediate ADCC alone and in combination in an autologous model was not improved.CONCLUSION:: HAART improves the ability of NK cells to mediate ADCC after six months. This improvement does not correlate with general immune restoration, as measured by CD4 T cell counts, but rather to a decrease in the frequency of NK cells expressing CCR7 and CD27.",
author = "Jensen, {Sanne Skov} and Hartling, {Hans Jakob} and Tingstedt, {Jeanette Linnea} and Tine Larsen and Nielsen, {Susanne Dam} and Court Pedersen and Anders Fomsgaard and Ingrid Karlsson",
year = "2015",
doi = "10.1097/QAI.0000000000000429",
language = "English",
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HIV-specific ADCC improves after antiretroviral therapy and correlates with normalization of the NK cell phenotype. / Jensen, Sanne Skov; Hartling, Hans Jakob; Tingstedt, Jeanette Linnea; Larsen, Tine ; Nielsen, Susanne Dam; Pedersen, Court; Fomsgaard, Anders; Karlsson, Ingrid.

I: J A I D S, Bind 68, Nr. 2, 2015, s. 103-111.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - HIV-specific ADCC improves after antiretroviral therapy and correlates with normalization of the NK cell phenotype

AU - Jensen, Sanne Skov

AU - Hartling, Hans Jakob

AU - Tingstedt, Jeanette Linnea

AU - Larsen, Tine

AU - Nielsen, Susanne Dam

AU - Pedersen, Court

AU - Fomsgaard, Anders

AU - Karlsson, Ingrid

PY - 2015

Y1 - 2015

N2 - BACKGROUND:: Natural killer (NK) cell phenotype and function have recently gained much attention as playing crucial roles in antibody-dependent cellular cytotoxicity (ADCC). We investigated NK cell function, as measured by ADCC, in HIV-1 positive individuals before and six months after highly active antiretroviral therapy (HAART) initiation.METHOD:: The ability of antibodies and NK cells to mediate ADCC was investigated separately and in combination in an autologous model. The NK cell subset distribution and NK cell phenotype (i.e. expression of maturation and activation markers within NK cell subsets) were analyzed.RESULTS:: The ability of NK cells to mediate ADCC was significantly increased after only six months of HAART and was not explained by a normalization of NK cell subsets (CD56CD16 and CD56CD16 NK cells) but rather by normalization in the frequency of NK cells expressing CCR7 and CD27. For individuals with no increase in ADCC after six months of HAART, the frequency of NK cells expressing NKp46 was down-regulated. The ability of antibodies to mediate ADCC alone and in combination in an autologous model was not improved.CONCLUSION:: HAART improves the ability of NK cells to mediate ADCC after six months. This improvement does not correlate with general immune restoration, as measured by CD4 T cell counts, but rather to a decrease in the frequency of NK cells expressing CCR7 and CD27.

AB - BACKGROUND:: Natural killer (NK) cell phenotype and function have recently gained much attention as playing crucial roles in antibody-dependent cellular cytotoxicity (ADCC). We investigated NK cell function, as measured by ADCC, in HIV-1 positive individuals before and six months after highly active antiretroviral therapy (HAART) initiation.METHOD:: The ability of antibodies and NK cells to mediate ADCC was investigated separately and in combination in an autologous model. The NK cell subset distribution and NK cell phenotype (i.e. expression of maturation and activation markers within NK cell subsets) were analyzed.RESULTS:: The ability of NK cells to mediate ADCC was significantly increased after only six months of HAART and was not explained by a normalization of NK cell subsets (CD56CD16 and CD56CD16 NK cells) but rather by normalization in the frequency of NK cells expressing CCR7 and CD27. For individuals with no increase in ADCC after six months of HAART, the frequency of NK cells expressing NKp46 was down-regulated. The ability of antibodies to mediate ADCC alone and in combination in an autologous model was not improved.CONCLUSION:: HAART improves the ability of NK cells to mediate ADCC after six months. This improvement does not correlate with general immune restoration, as measured by CD4 T cell counts, but rather to a decrease in the frequency of NK cells expressing CCR7 and CD27.

U2 - 10.1097/QAI.0000000000000429

DO - 10.1097/QAI.0000000000000429

M3 - Journal article

C2 - 25394194

VL - 68

SP - 103

EP - 111

JO - J A I D S

JF - J A I D S

SN - 1525-4135

IS - 2

ER -