High–performance liquid chromatography of clomipramine and metabolites in human plasma and urine

Karin Kramer Nielsen*, Kim Brøsen

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

An isocratic, high-performance liquid chromatography method has been developed for simultaneous determination of the tricyclic antidepressant clomipramine and six metabolites: desmethylclomipramine, 2- and 8-hydroxy-clomipramine, 2- and 8-hydroxydesmethylclomipramine and didesmethyl-clomipramine in plasma and urine. In addition, clomipramine N-oxide was determined in the unconjugated fraction of urine. The method is based on a three-step liquid-liquid extraction, the chromatographic eluent was 30% acetonitrile and 70% aqueous sodium perchlorate solution, pH 2.5, and the UV detection was performed at 220 nm. The method was applied to the analysis of samples followed by oral doses of clomipramine to patients and healthy volunteers.

OriginalsprogEngelsk
TidsskriftTherapeutic Drug Monitoring
Vol/bind15
Udgave nummer2
Sider (fra-til)122-128
Antal sider7
ISSN0163-4356
DOI
StatusUdgivet - 1. jan. 1993

    Fingerprint

Citationsformater