High temperatures cause reduced growth, plant death and metabolic changes in eelgrass Zostera marina

Kathrine Jul Hammer, Jens Borum*, Harald Hasler-Sheetal, Erin C. Shields, Kaj Sand-Jensen, Kenneth A. Moore

*Kontaktforfatter for dette arbejde

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Resumé

We investigated temperature effects on eelgrass Zostera marina L. growing close to its southern distribution limit along the eastern coast of North America in Virginia, USA. We combined growth and survival experiments with microelectrode measurements of internal meristematic oxygen and analyses of metabolic compounds. Eelgrass shoots were grown at 3 different temperatures (22, 26 and 30°C) and field equivalent light levels (23% of sea surface insolation) for 28 d while water column oxygen concentration was kept at air saturation. Meristematic oxygen concentrations did not vary significantly with temperature, and meristems maintained a relatively high oxygen concentration (average: 38% air saturation) during dark hours. Despite high meristematic oxygen concentrations, shoots growing at 30°C exhibited increased mortality, reduced growth and reduced leaf production compared to shoots growing at 22 and 26°C. The leaf metabolome was significantly altered at 30°C, indicating an increase of reactive oxygen species. In addition, total nitrogen and metabolites related to the nitrogen cycle (amino acids, urea and γ-aminobutyric acid [GABA]) were low in the heat-stressed shoots, whereas soluble sugars increased. In conclusion, high temperature (30°C) has strong negative effects on eelgrass in the lower Chesapeake Bay, affecting growth, tissue integrity, nitrogen metabolism and protein/enzyme synthesis. Future global warming may likely deteriorate populations of eelgrass at its present southern distribution limit.

OriginalsprogEngelsk
TidsskriftMarine Ecology Progress Series
Vol/bind604
Sider (fra-til)121-132
ISSN0171-8630
DOI
StatusUdgivet - okt. 2018

Fingeraftryk

Zostera marina
marina
plant growth
death
oxygen
shoot
shoots
temperature
saturation
metabolome
air
nitrogen cycle
nitrogen
gamma-aminobutyric acid
Chesapeake Bay
nitrogen metabolism
insolation
temperature effect
meristems
global warming

Citer dette

Hammer, Kathrine Jul ; Borum, Jens ; Hasler-Sheetal, Harald ; Shields, Erin C. ; Sand-Jensen, Kaj ; Moore, Kenneth A. / High temperatures cause reduced growth, plant death and metabolic changes in eelgrass Zostera marina. I: Marine Ecology Progress Series. 2018 ; Bind 604. s. 121-132.
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title = "High temperatures cause reduced growth, plant death and metabolic changes in eelgrass Zostera marina",
abstract = "We investigated temperature effects on eelgrass Zostera marina L. growing close to its southern distribution limit along the eastern coast of North America in Virginia, USA. We combined growth and survival experiments with microelectrode measurements of internal meristematic oxygen and analyses of metabolic compounds. Eelgrass shoots were grown at 3 different temperatures (22, 26 and 30°C) and field equivalent light levels (23{\%} of sea surface insolation) for 28 d while water column oxygen concentration was kept at air saturation. Meristematic oxygen concentrations did not vary significantly with temperature, and meristems maintained a relatively high oxygen concentration (average: 38{\%} air saturation) during dark hours. Despite high meristematic oxygen concentrations, shoots growing at 30°C exhibited increased mortality, reduced growth and reduced leaf production compared to shoots growing at 22 and 26°C. The leaf metabolome was significantly altered at 30°C, indicating an increase of reactive oxygen species. In addition, total nitrogen and metabolites related to the nitrogen cycle (amino acids, urea and γ-aminobutyric acid [GABA]) were low in the heat-stressed shoots, whereas soluble sugars increased. In conclusion, high temperature (30°C) has strong negative effects on eelgrass in the lower Chesapeake Bay, affecting growth, tissue integrity, nitrogen metabolism and protein/enzyme synthesis. Future global warming may likely deteriorate populations of eelgrass at its present southern distribution limit.",
keywords = "Climate change, Growth, Metabolomics, Temperature stress, Zostera marina",
author = "Hammer, {Kathrine Jul} and Jens Borum and Harald Hasler-Sheetal and Shields, {Erin C.} and Kaj Sand-Jensen and Moore, {Kenneth A.}",
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High temperatures cause reduced growth, plant death and metabolic changes in eelgrass Zostera marina. / Hammer, Kathrine Jul; Borum, Jens; Hasler-Sheetal, Harald; Shields, Erin C.; Sand-Jensen, Kaj; Moore, Kenneth A.

I: Marine Ecology Progress Series, Bind 604, 10.2018, s. 121-132.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - High temperatures cause reduced growth, plant death and metabolic changes in eelgrass Zostera marina

AU - Hammer, Kathrine Jul

AU - Borum, Jens

AU - Hasler-Sheetal, Harald

AU - Shields, Erin C.

AU - Sand-Jensen, Kaj

AU - Moore, Kenneth A.

PY - 2018/10

Y1 - 2018/10

N2 - We investigated temperature effects on eelgrass Zostera marina L. growing close to its southern distribution limit along the eastern coast of North America in Virginia, USA. We combined growth and survival experiments with microelectrode measurements of internal meristematic oxygen and analyses of metabolic compounds. Eelgrass shoots were grown at 3 different temperatures (22, 26 and 30°C) and field equivalent light levels (23% of sea surface insolation) for 28 d while water column oxygen concentration was kept at air saturation. Meristematic oxygen concentrations did not vary significantly with temperature, and meristems maintained a relatively high oxygen concentration (average: 38% air saturation) during dark hours. Despite high meristematic oxygen concentrations, shoots growing at 30°C exhibited increased mortality, reduced growth and reduced leaf production compared to shoots growing at 22 and 26°C. The leaf metabolome was significantly altered at 30°C, indicating an increase of reactive oxygen species. In addition, total nitrogen and metabolites related to the nitrogen cycle (amino acids, urea and γ-aminobutyric acid [GABA]) were low in the heat-stressed shoots, whereas soluble sugars increased. In conclusion, high temperature (30°C) has strong negative effects on eelgrass in the lower Chesapeake Bay, affecting growth, tissue integrity, nitrogen metabolism and protein/enzyme synthesis. Future global warming may likely deteriorate populations of eelgrass at its present southern distribution limit.

AB - We investigated temperature effects on eelgrass Zostera marina L. growing close to its southern distribution limit along the eastern coast of North America in Virginia, USA. We combined growth and survival experiments with microelectrode measurements of internal meristematic oxygen and analyses of metabolic compounds. Eelgrass shoots were grown at 3 different temperatures (22, 26 and 30°C) and field equivalent light levels (23% of sea surface insolation) for 28 d while water column oxygen concentration was kept at air saturation. Meristematic oxygen concentrations did not vary significantly with temperature, and meristems maintained a relatively high oxygen concentration (average: 38% air saturation) during dark hours. Despite high meristematic oxygen concentrations, shoots growing at 30°C exhibited increased mortality, reduced growth and reduced leaf production compared to shoots growing at 22 and 26°C. The leaf metabolome was significantly altered at 30°C, indicating an increase of reactive oxygen species. In addition, total nitrogen and metabolites related to the nitrogen cycle (amino acids, urea and γ-aminobutyric acid [GABA]) were low in the heat-stressed shoots, whereas soluble sugars increased. In conclusion, high temperature (30°C) has strong negative effects on eelgrass in the lower Chesapeake Bay, affecting growth, tissue integrity, nitrogen metabolism and protein/enzyme synthesis. Future global warming may likely deteriorate populations of eelgrass at its present southern distribution limit.

KW - Climate change

KW - Growth

KW - Metabolomics

KW - Temperature stress

KW - Zostera marina

U2 - 10.3354/meps12740

DO - 10.3354/meps12740

M3 - Journal article

AN - SCOPUS:85054825388

VL - 604

SP - 121

EP - 132

JO - Marine Ecology - Progress Series

JF - Marine Ecology - Progress Series

SN - 0171-8630

ER -