High-performance liquid chromatography of imipramine and six metabolites in human plasma and urine

Karin Kramer Nielsen*, Kim Brøsen

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Abstrakt

A method for the simultaneous quantitation of imipramine and six metabolites (2- and 10-hydroxyimipramine, 2- and 10-hydroxydesipramine, didesmethylimipramine and desipramine) in human plasma and urine has been developed. The method is based on a three-step liquid-liquid extraction followed by isocratic, reversed-phase high-performance liquid chromatography with ultraviolet absorbance detection (detection wavelength: 220 nm). The chromatographic eluent consisted of 30% acetonitrile and 70% aqueous sodium perchlorate solution pH 2.5. Glucuronide conjugates in urine were deconjugated with β-glucuronidase/arylsulphatase prior to extraction.

OriginalsprogEngelsk
TidsskriftJournal of Chromatography B: Biomedical Sciences and Applications
Vol/bind612
Udgave nummer1
Sider (fra-til)87-94
Antal sider8
ISSN0378-4347
DOI
StatusUdgivet - 29. jan. 1993

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