Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

Tine Rosenberg, Charlotte Aaberg-Jessen, Stine Asferg Petterson, Bjarne Winther Kristensen

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Resumé

AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.

MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.

RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.

CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

OriginalsprogEngelsk
ArtikelnummerCNS15
TidsskriftC N S Oncology
Vol/bind7
Udgave nummer2
Antal sider14
ISSN2045-0907
DOI
StatusUdgivet - apr. 2018

Fingeraftryk

Glioblastoma
Nestin
Vascular Endothelial Growth Factor A
Oxygen

Citer dette

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title = "Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia",
abstract = "AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2{\%} or 21{\%} oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.",
keywords = "cancer stem cells, glioblastoma, glioblastoma subtypes, hypoxia, spheroid cultures, stem cell markers",
author = "Tine Rosenberg and Charlotte Aaberg-Jessen and Petterson, {Stine Asferg} and Kristensen, {Bjarne Winther}",
year = "2018",
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Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia. / Rosenberg, Tine; Aaberg-Jessen, Charlotte; Petterson, Stine Asferg; Kristensen, Bjarne Winther.

I: C N S Oncology, Bind 7, Nr. 2, CNS15, 04.2018.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Heterogenic expression of stem cell markers in patient-derived glioblastoma spheroid cultures exposed to long-term hypoxia

AU - Rosenberg, Tine

AU - Aaberg-Jessen, Charlotte

AU - Petterson, Stine Asferg

AU - Kristensen, Bjarne Winther

PY - 2018/4

Y1 - 2018/4

N2 - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

AB - AIM: To investigate the time profile of hypoxia and stem cell markers in glioblastoma spheroids of known molecular subtype.MATERIALS & METHODS: Patient-derived glioblastoma spheroids were cultured up to 7 days in either 2% or 21% oxygen. Levels of proliferation (Ki-67), hypoxia (HIF-1α, CA9 and VEGF) and stem cell markers (CD133, nestin and musashi-1) were investigated by immunohistochemistry.RESULTS: Hypoxia markers as well as CD133 and partially nestin increased in long-term hypoxia. The proliferation rate and spheroid size were highest in normoxia.CONCLUSION: We found differences in hypoxia and stem cell marker profiles between the patient-derived glioblastoma cultures. This heterogeneity should be taken into consideration in development of future therapeutic strategies.

KW - cancer stem cells

KW - glioblastoma

KW - glioblastoma subtypes

KW - hypoxia

KW - spheroid cultures

KW - stem cell markers

U2 - 10.2217/cns-2017-0034

DO - 10.2217/cns-2017-0034

M3 - Journal article

C2 - 29708435

VL - 7

JO - C N S Oncology

JF - C N S Oncology

SN - 2045-0907

IS - 2

M1 - CNS15

ER -