Resumé

Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.
OriginalsprogEngelsk
Publikationsdato11. jun. 2018
StatusUdgivet - 11. jun. 2018
Begivenhed2nd international HeFIB conference on Helium and emerging Focused Ion Beams - Salzgasse, 4, 01067 , Dresden, Tyskland
Varighed: 11. jun. 201813. jun. 2018
https://www.hzdr.de/db/Cms?pNid=3699

Konference

Konference2nd international HeFIB conference on Helium and emerging Focused Ion Beams
LokationSalzgasse, 4, 01067
LandTyskland
ByDresden
Periode11/06/201813/06/2018
Internetadresse

Fingeraftryk

helium ions
microscopy
drying
critical point
ion microscopes
low currents
ion sources
embedding
helium
preparation
high resolution

Citer dette

Leißner, T., Tavares, L., Gustafsson, L. R., Rubahn, H-G., Kjelstrup-Hansen, J., & Marcussen, N. (2018). Helium Ion Microscopy for the imaging of biological samples. Poster session præsenteret på 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Tyskland.
Leißner, Till ; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter ; Kjelstrup-Hansen, Jakob ; Marcussen, Niels. / Helium Ion Microscopy for the imaging of biological samples. Poster session præsenteret på 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Tyskland.
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title = "Helium Ion Microscopy for the imaging of biological samples",
abstract = "Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.",
author = "Till Lei{\ss}ner and Luciana Tavares and Gustafsson, {Linnea Rebecka} and Horst-G{\"u}nter Rubahn and Jakob Kjelstrup-Hansen and Niels Marcussen",
year = "2018",
month = "6",
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language = "English",
note = "2nd international HeFIB conference on Helium and emerging Focused Ion Beams, HeFIB 2018 ; Conference date: 11-06-2018 Through 13-06-2018",
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Leißner, T, Tavares, L, Gustafsson, LR, Rubahn, H-G, Kjelstrup-Hansen, J & Marcussen, N 2018, 'Helium Ion Microscopy for the imaging of biological samples' 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Tyskland, 11/06/2018 - 13/06/2018, .

Helium Ion Microscopy for the imaging of biological samples. / Leißner, Till; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter; Kjelstrup-Hansen, Jakob; Marcussen, Niels.

2018. Poster session præsenteret på 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Tyskland.

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

TY - CONF

T1 - Helium Ion Microscopy for the imaging of biological samples

AU - Leißner, Till

AU - Tavares, Luciana

AU - Gustafsson, Linnea Rebecka

AU - Rubahn, Horst-Günter

AU - Kjelstrup-Hansen, Jakob

AU - Marcussen, Niels

PY - 2018/6/11

Y1 - 2018/6/11

N2 - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

AB - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

M3 - Poster

ER -

Leißner T, Tavares L, Gustafsson LR, Rubahn H-G, Kjelstrup-Hansen J, Marcussen N. Helium Ion Microscopy for the imaging of biological samples. 2018. Poster session præsenteret på 2nd international HeFIB conference on Helium and emerging Focused Ion Beams, Dresden, Tyskland.