Helium Ion Microscopy for the imaging of biological samples

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Resumé

Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.
OriginalsprogEngelsk
Publikationsdato31. maj 2018
StatusUdgivet - 31. maj 2018
BegivenhedInternational Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018 - University of Southern Denmark, Sønderborg, Danmark
Varighed: 30. maj 20181. jun. 2018
https://www.sdu.dk/en/nibs2018

Konference

KonferenceInternational Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018
LokationUniversity of Southern Denmark
LandDanmark
BySønderborg
Periode30/05/201801/06/2018
Internetadresse

Fingeraftryk

helium ions
microscopy
drying
critical point
ion microscopes
low currents
ion sources
embedding
helium
preparation
high resolution

Citer dette

Leißner, T., Tavares, L., Gustafsson, L. R., Rubahn, H-G., Kjelstrup-Hansen, J., & Marcussen, N. (2018). Helium Ion Microscopy for the imaging of biological samples. Poster session præsenteret på International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Danmark.
Leißner, Till ; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter ; Kjelstrup-Hansen, Jakob ; Marcussen, Niels. / Helium Ion Microscopy for the imaging of biological samples. Poster session præsenteret på International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Danmark.
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title = "Helium Ion Microscopy for the imaging of biological samples",
abstract = "Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.",
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Leißner, T, Tavares, L, Gustafsson, LR, Rubahn, H-G, Kjelstrup-Hansen, J & Marcussen, N 2018, 'Helium Ion Microscopy for the imaging of biological samples' International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Danmark, 30/05/2018 - 01/06/2018, .

Helium Ion Microscopy for the imaging of biological samples. / Leißner, Till; Tavares, Luciana ; Gustafsson, Linnea Rebecka ; Rubahn, Horst-Günter; Kjelstrup-Hansen, Jakob; Marcussen, Niels.

2018. Poster session præsenteret på International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Danmark.

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

TY - CONF

T1 - Helium Ion Microscopy for the imaging of biological samples

AU - Leißner, Till

AU - Tavares, Luciana

AU - Gustafsson, Linnea Rebecka

AU - Rubahn, Horst-Günter

AU - Kjelstrup-Hansen, Jakob

AU - Marcussen, Niels

PY - 2018/5/31

Y1 - 2018/5/31

N2 - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

AB - Imaging of biological samples benefits from several unique capabilities of the Helium ion microscope, e.g. charge compensation, large depth of focus, and sequential milling and imaging obtained by combining the different ion sources for investigation of different tissue layers. In this work we investigate the influence of the sample preparation procedures of biological samples on the resulting imaging quality. We tested various fixatives and embedding procedures and investigated the impact of critical point drying. We find that using glutaraldehyde as a fixative combined with critical point drying of the sample preserve the tissue of shrinking and allows imaging the sample with low-current Helium beams at high resolution.

M3 - Poster

ER -

Leißner T, Tavares L, Gustafsson LR, Rubahn H-G, Kjelstrup-Hansen J, Marcussen N. Helium Ion Microscopy for the imaging of biological samples. 2018. Poster session præsenteret på International Conference on Nanotechnology and Innovation in the Baltic Sea Region 2018, Sønderborg, Danmark.