Growth and development of the placenta in the capybara (Hydrochaeris hydrochaeris)

Claudia Kanashiro, Tatiana C Santos, Maria Angelica Miglino, Andrea M Mess, Anthony M Carter

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2009-null
OriginalsprogEngelsk
TidsskriftReproductive Biology and Endocrinology
Vol/bind7
Sider (fra-til)57
Antal sider13
ISSN1477-7827
DOI
StatusUdgivet - 1. jan. 2009

Fingeraftryk

Trophoblasts
Growth and Development
Placenta
Rodentia
Placentation
Limb Buds
Mesoderm
Latex
Guinea Pigs
Mothers
Proliferating Cell Nuclear Antigen
Vimentin
Growth
Transmission Electron Microscopy
Smooth Muscle
Actins
Cell Proliferation

Citer dette

Kanashiro, Claudia ; Santos, Tatiana C ; Miglino, Maria Angelica ; Mess, Andrea M ; Carter, Anthony M. / Growth and development of the placenta in the capybara (Hydrochaeris hydrochaeris). I: Reproductive Biology and Endocrinology. 2009 ; Bind 7. s. 57.
@article{0fa3de10d43b11deb0fe000ea68e967b,
title = "Growth and development of the placenta in the capybara (Hydrochaeris hydrochaeris)",
abstract = "BACKGROUND: The guinea pig is an attractive model for human pregnancy and placentation, mainly because of its haemomonochorial placental type, but is rather small in size. Therefore, to better understand the impact of body mass, we studied placental development in the capybara which has a body mass around 50 kg and a gestation period of around 150 days. We paid attention to the development of the lobulated arrangement of the placenta, the growth of the labyrinth in the course of gestation, the differentiation of the subplacenta, and the pattern of invasion by extraplacental trophoblast. METHODS: Material was collected from six animals at pregnancy stages ranging from the late limb bud stage to mid gestation. Methods included latex casts, standard histology, immunohistochemistry for cytokeratin, vimentin, alpha-smooth muscle actin, and proliferating cell nuclear antigen as well as transmission electron microscopy. RESULTS: At the limb bud stage, the placenta was a pad of trophoblast covered by a layer of mesoderm from which fetal vessels were beginning to penetrate at folds in the surface. By 70 days, the placenta comprised areas of labyrinth (lobes) separated by interlobular areas. Placental growth resulted predominantly from proliferation of cellular trophoblast situated in nests at the fetal side of the placenta and along internally directed projections on fetal mesenchyme. Additional proliferation was demonstrated for cellular trophoblast within the labyrinth.Already at the limb bud stage, there was a prominent subplacenta comprising cellular and syncytial trophoblast with mesenchyme and associated blood vessels. At 90 days, differentiation was complete and similar to that seen in other hystricognath rodents. Overlap of fetal vessels and maternal blood lacunae was confirmed by latex injection of the vessels. At all stages extraplacental trophoblast was associated with the maternal arterial supply and consisted of cellular trophoblast and syncytial streamers derived from the subplacenta. CONCLUSION: All important characteristics of placental development and organization in the capybara resembled those found in smaller hystricognath rodents including the guinea pig. These features apparently do not dependent on body size. Clearly, placentation in hystricognaths adheres to an extraordinarily stable pattern suggesting they can be used interchangeably as models of human placenta.",
keywords = "Animals, Body Size, Chorioallantoic Membrane, Decidua, Embryo Implantation, Female, Gestational Age, Limb Buds, Models, Animal, Placenta, Pregnancy, Pregnancy, Animal, Rodentia, Yolk Sac",
author = "Claudia Kanashiro and Santos, {Tatiana C} and Miglino, {Maria Angelica} and Mess, {Andrea M} and Carter, {Anthony M}",
year = "2009",
month = "1",
day = "1",
doi = "10.1186/1477-7827-7-57",
language = "English",
volume = "7",
pages = "57",
journal = "Reproductive Biology and Endocrinology",
issn = "1477-7827",
publisher = "BioMed Central",

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Growth and development of the placenta in the capybara (Hydrochaeris hydrochaeris). / Kanashiro, Claudia; Santos, Tatiana C; Miglino, Maria Angelica; Mess, Andrea M; Carter, Anthony M.

I: Reproductive Biology and Endocrinology, Bind 7, 01.01.2009, s. 57.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Growth and development of the placenta in the capybara (Hydrochaeris hydrochaeris)

AU - Kanashiro, Claudia

AU - Santos, Tatiana C

AU - Miglino, Maria Angelica

AU - Mess, Andrea M

AU - Carter, Anthony M

PY - 2009/1/1

Y1 - 2009/1/1

N2 - BACKGROUND: The guinea pig is an attractive model for human pregnancy and placentation, mainly because of its haemomonochorial placental type, but is rather small in size. Therefore, to better understand the impact of body mass, we studied placental development in the capybara which has a body mass around 50 kg and a gestation period of around 150 days. We paid attention to the development of the lobulated arrangement of the placenta, the growth of the labyrinth in the course of gestation, the differentiation of the subplacenta, and the pattern of invasion by extraplacental trophoblast. METHODS: Material was collected from six animals at pregnancy stages ranging from the late limb bud stage to mid gestation. Methods included latex casts, standard histology, immunohistochemistry for cytokeratin, vimentin, alpha-smooth muscle actin, and proliferating cell nuclear antigen as well as transmission electron microscopy. RESULTS: At the limb bud stage, the placenta was a pad of trophoblast covered by a layer of mesoderm from which fetal vessels were beginning to penetrate at folds in the surface. By 70 days, the placenta comprised areas of labyrinth (lobes) separated by interlobular areas. Placental growth resulted predominantly from proliferation of cellular trophoblast situated in nests at the fetal side of the placenta and along internally directed projections on fetal mesenchyme. Additional proliferation was demonstrated for cellular trophoblast within the labyrinth.Already at the limb bud stage, there was a prominent subplacenta comprising cellular and syncytial trophoblast with mesenchyme and associated blood vessels. At 90 days, differentiation was complete and similar to that seen in other hystricognath rodents. Overlap of fetal vessels and maternal blood lacunae was confirmed by latex injection of the vessels. At all stages extraplacental trophoblast was associated with the maternal arterial supply and consisted of cellular trophoblast and syncytial streamers derived from the subplacenta. CONCLUSION: All important characteristics of placental development and organization in the capybara resembled those found in smaller hystricognath rodents including the guinea pig. These features apparently do not dependent on body size. Clearly, placentation in hystricognaths adheres to an extraordinarily stable pattern suggesting they can be used interchangeably as models of human placenta.

AB - BACKGROUND: The guinea pig is an attractive model for human pregnancy and placentation, mainly because of its haemomonochorial placental type, but is rather small in size. Therefore, to better understand the impact of body mass, we studied placental development in the capybara which has a body mass around 50 kg and a gestation period of around 150 days. We paid attention to the development of the lobulated arrangement of the placenta, the growth of the labyrinth in the course of gestation, the differentiation of the subplacenta, and the pattern of invasion by extraplacental trophoblast. METHODS: Material was collected from six animals at pregnancy stages ranging from the late limb bud stage to mid gestation. Methods included latex casts, standard histology, immunohistochemistry for cytokeratin, vimentin, alpha-smooth muscle actin, and proliferating cell nuclear antigen as well as transmission electron microscopy. RESULTS: At the limb bud stage, the placenta was a pad of trophoblast covered by a layer of mesoderm from which fetal vessels were beginning to penetrate at folds in the surface. By 70 days, the placenta comprised areas of labyrinth (lobes) separated by interlobular areas. Placental growth resulted predominantly from proliferation of cellular trophoblast situated in nests at the fetal side of the placenta and along internally directed projections on fetal mesenchyme. Additional proliferation was demonstrated for cellular trophoblast within the labyrinth.Already at the limb bud stage, there was a prominent subplacenta comprising cellular and syncytial trophoblast with mesenchyme and associated blood vessels. At 90 days, differentiation was complete and similar to that seen in other hystricognath rodents. Overlap of fetal vessels and maternal blood lacunae was confirmed by latex injection of the vessels. At all stages extraplacental trophoblast was associated with the maternal arterial supply and consisted of cellular trophoblast and syncytial streamers derived from the subplacenta. CONCLUSION: All important characteristics of placental development and organization in the capybara resembled those found in smaller hystricognath rodents including the guinea pig. These features apparently do not dependent on body size. Clearly, placentation in hystricognaths adheres to an extraordinarily stable pattern suggesting they can be used interchangeably as models of human placenta.

KW - Animals

KW - Body Size

KW - Chorioallantoic Membrane

KW - Decidua

KW - Embryo Implantation

KW - Female

KW - Gestational Age

KW - Limb Buds

KW - Models, Animal

KW - Placenta

KW - Pregnancy

KW - Pregnancy, Animal

KW - Rodentia

KW - Yolk Sac

U2 - 10.1186/1477-7827-7-57

DO - 10.1186/1477-7827-7-57

M3 - Journal article

VL - 7

SP - 57

JO - Reproductive Biology and Endocrinology

JF - Reproductive Biology and Endocrinology

SN - 1477-7827

ER -