@article{5bdfeb00fefb11dc86ef000ea68e967b,
title = "Glutamate receptor antagonists and growth factors modulate dentate granule cell neurogenesis in organotypic, rat hippocampal slice cultures.",
abstract = "Generation of dentate granule cells and its modulation by glutamate receptor antagonists, growth factors and pilocarpine-induced seizure-like activity was investigated in rat hippocampal slice cultures derived from 1-week-old rats and grown for 2 weeks. Focussing on the dentate granule cell layer facing CA1 and the immediate subgranular zone, exposure for 3 days to the NMDA receptor blocking agents MK-801 (10 microM) or APV (25 microM) in the culture medium, increased the number of TOAD-64/Ulip/CRMP-4 (TUC-4)-positive cells as counted in the slice cultures at the end of the 3-day treatment period. Exposure to IGF-I (200 ng/ml) and EGF (20 ng/ml) also increased the number of TUC-4-positive cells. Combining APV with IGF-I/EGF had an additive effect. Similar results were obtained by 3 days treatment with the AMPA receptor antagonist CNQX (25 microM). Surprisingly, addition of 5 mM pilocarpine reduced the number of TUC-4-positive cells, just as combining pilocarpine with the neurogenesis-stimulating compounds, prevented or reduced the increase of TUC-4-positive cells. None of the treatments were found to induce dentate granule cell death within the observed period. Labeling of dividing cells by adding 5-bromo-2-deoxyuridine (BrdU) to the culture medium did not result in cells double-labeled with BrdU and TUC-4. The induced increase in TUC-4-positive cells therefore represent neuronal differentiation of existing neural precursor cells when investigated at the 3-day time point. We conclude that 3 days treatment of 2-week-old hippocampal slice cultures with IGF-I and EGF and NMDA and AMPA glutamate receptor antagonists increase granule cell neurogenesis from preexisting neural precursors.",
keywords = "Animals, Cell Differentiation, Dentate Gyrus, Epidermal Growth Factor, Excitatory Amino Acid Antagonists, Insulin-Like Growth Factor I, Nerve Tissue Proteins, Neurons, Organ Culture Techniques, Pilocarpine, Rats, Rats, Wistar, Receptors, Glutamate, Seizures, Stem Cells",
author = "Poulsen, \{Frantz Rom\} and Morten Blaabjerg and Maria Montero and Jens Zimmer",
year = "2005",
month = jul,
day = "27",
doi = "10.1016/j.brainres.2005.05.050",
language = "English",
volume = "1051",
pages = "35--49",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",
}