Glucagon-like peptide-2 induces rapid digestive adaptation following intestinal resection in preterm neonates

Andreas Vegge, Thomas Thymann, Pernille Lund, Barbara Stoll, Stine B Bering, Bolette Hartmann, Jacob Jelsing, Niels Qvist, Douglas G Burrin, Palle B Jeppesen, Jens J Holst, Per T Sangild

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    Resumé

    Short bowel syndrome (SBS) is a frequent complication after intestinal resection in infants suffering from intestinal disease. We tested whether treatment with the intestinotrophic hormone glucagon-like peptide-2 (GLP-2) increases intestinal volume and function in the period immediately following intestinal resection in preterm pigs. Preterm pigs were fed enterally for 48 h before undergoing resection of 50% of the small intestine and establishment of a jejunostomy. Following resection, pigs were maintained on total parenteral nutrition (TPN) without (SBS, n = 8) or with GLP-2 treatment (3.5 μg/kg body wt per h, SBS+GLP-2, n = 7) and compared with a group of unresected preterm pigs (control, n = 5). After 5 days of TPN, all piglets were fed enterally for 24 h, and a nutrient balance study was performed. Intestinal resection was associated with markedly reduced endogenous GLP-2 levels. GLP-2 increased the relative absorption of wet weight (46 vs. 22%), energy (79 vs. 64%), and all macronutrients (all parameters P <0.05). These findings were supported by a 200% increase in sucrase and maltase activities, a 50% increase in small intestinal epithelial volume (P <0.05), as well as increased DNA and protein contents and increased total protein synthesis rate in SBS+GLP-2 vs. SBS pigs (+100%, P <0.05). Following intestinal resection in preterm pigs, GLP-2 induced structural and functional adaptation, resulting in a higher relative absorption of fluid and macronutrients. GLP-2 treatment may be a promising therapy to enhance intestinal adaptation and improve digestive function in preterm infants with jejunostomy following intestinal resection.
    OriginalsprogEngelsk
    ArtikelnummerG277-G285
    TidsskriftA J P: Gastrointestinal and Liver Physiology (Online)
    Vol/bind305
    Antal sider9
    ISSN1522-1547
    DOI
    StatusUdgivet - 2013

    Fingeraftryk

    Glucagon-Like Peptide 2
    Short Bowel Syndrome
    Newborn Infant
    Jejunostomy
    Intestinal Diseases
    alpha-Glucosidases
    Premature Infants
    Small Intestine
    Proteins
    Hormones
    Weights and Measures
    Food

    Citer dette

    Vegge, Andreas ; Thymann, Thomas ; Lund, Pernille ; Stoll, Barbara ; Bering, Stine B ; Hartmann, Bolette ; Jelsing, Jacob ; Qvist, Niels ; Burrin, Douglas G ; Jeppesen, Palle B ; Holst, Jens J ; Sangild, Per T. / Glucagon-like peptide-2 induces rapid digestive adaptation following intestinal resection in preterm neonates. I: A J P: Gastrointestinal and Liver Physiology (Online). 2013 ; Bind 305.
    @article{c5e98bb8eaea4decaff59f1667c24aff,
    title = "Glucagon-like peptide-2 induces rapid digestive adaptation following intestinal resection in preterm neonates",
    abstract = "Short bowel syndrome (SBS) is a frequent complication after intestinal resection in infants suffering from intestinal disease. We tested whether treatment with the intestinotrophic hormone glucagon-like peptide-2 (GLP-2) increases intestinal volume and function in the period immediately following intestinal resection in preterm pigs. Preterm pigs were fed enterally for 48 h before undergoing resection of 50{\%} of the small intestine and establishment of a jejunostomy. Following resection, pigs were maintained on total parenteral nutrition (TPN) without (SBS, n = 8) or with GLP-2 treatment (3.5 μg/kg body wt per h, SBS+GLP-2, n = 7) and compared with a group of unresected preterm pigs (control, n = 5). After 5 days of TPN, all piglets were fed enterally for 24 h, and a nutrient balance study was performed. Intestinal resection was associated with markedly reduced endogenous GLP-2 levels. GLP-2 increased the relative absorption of wet weight (46 vs. 22{\%}), energy (79 vs. 64{\%}), and all macronutrients (all parameters P <0.05). These findings were supported by a 200{\%} increase in sucrase and maltase activities, a 50{\%} increase in small intestinal epithelial volume (P <0.05), as well as increased DNA and protein contents and increased total protein synthesis rate in SBS+GLP-2 vs. SBS pigs (+100{\%}, P <0.05). Following intestinal resection in preterm pigs, GLP-2 induced structural and functional adaptation, resulting in a higher relative absorption of fluid and macronutrients. GLP-2 treatment may be a promising therapy to enhance intestinal adaptation and improve digestive function in preterm infants with jejunostomy following intestinal resection.",
    author = "Andreas Vegge and Thomas Thymann and Pernille Lund and Barbara Stoll and Bering, {Stine B} and Bolette Hartmann and Jacob Jelsing and Niels Qvist and Burrin, {Douglas G} and Jeppesen, {Palle B} and Holst, {Jens J} and Sangild, {Per T}",
    year = "2013",
    doi = "10.1152/ajpgi.00064.2013",
    language = "English",
    volume = "305",
    journal = "A J P: Gastrointestinal and Liver Physiology (Online)",
    issn = "1522-1547",
    publisher = "American Physiological Society",

    }

    Glucagon-like peptide-2 induces rapid digestive adaptation following intestinal resection in preterm neonates. / Vegge, Andreas; Thymann, Thomas; Lund, Pernille; Stoll, Barbara; Bering, Stine B; Hartmann, Bolette; Jelsing, Jacob; Qvist, Niels; Burrin, Douglas G; Jeppesen, Palle B; Holst, Jens J; Sangild, Per T.

    I: A J P: Gastrointestinal and Liver Physiology (Online), Bind 305, G277-G285, 2013.

    Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

    TY - JOUR

    T1 - Glucagon-like peptide-2 induces rapid digestive adaptation following intestinal resection in preterm neonates

    AU - Vegge, Andreas

    AU - Thymann, Thomas

    AU - Lund, Pernille

    AU - Stoll, Barbara

    AU - Bering, Stine B

    AU - Hartmann, Bolette

    AU - Jelsing, Jacob

    AU - Qvist, Niels

    AU - Burrin, Douglas G

    AU - Jeppesen, Palle B

    AU - Holst, Jens J

    AU - Sangild, Per T

    PY - 2013

    Y1 - 2013

    N2 - Short bowel syndrome (SBS) is a frequent complication after intestinal resection in infants suffering from intestinal disease. We tested whether treatment with the intestinotrophic hormone glucagon-like peptide-2 (GLP-2) increases intestinal volume and function in the period immediately following intestinal resection in preterm pigs. Preterm pigs were fed enterally for 48 h before undergoing resection of 50% of the small intestine and establishment of a jejunostomy. Following resection, pigs were maintained on total parenteral nutrition (TPN) without (SBS, n = 8) or with GLP-2 treatment (3.5 μg/kg body wt per h, SBS+GLP-2, n = 7) and compared with a group of unresected preterm pigs (control, n = 5). After 5 days of TPN, all piglets were fed enterally for 24 h, and a nutrient balance study was performed. Intestinal resection was associated with markedly reduced endogenous GLP-2 levels. GLP-2 increased the relative absorption of wet weight (46 vs. 22%), energy (79 vs. 64%), and all macronutrients (all parameters P <0.05). These findings were supported by a 200% increase in sucrase and maltase activities, a 50% increase in small intestinal epithelial volume (P <0.05), as well as increased DNA and protein contents and increased total protein synthesis rate in SBS+GLP-2 vs. SBS pigs (+100%, P <0.05). Following intestinal resection in preterm pigs, GLP-2 induced structural and functional adaptation, resulting in a higher relative absorption of fluid and macronutrients. GLP-2 treatment may be a promising therapy to enhance intestinal adaptation and improve digestive function in preterm infants with jejunostomy following intestinal resection.

    AB - Short bowel syndrome (SBS) is a frequent complication after intestinal resection in infants suffering from intestinal disease. We tested whether treatment with the intestinotrophic hormone glucagon-like peptide-2 (GLP-2) increases intestinal volume and function in the period immediately following intestinal resection in preterm pigs. Preterm pigs were fed enterally for 48 h before undergoing resection of 50% of the small intestine and establishment of a jejunostomy. Following resection, pigs were maintained on total parenteral nutrition (TPN) without (SBS, n = 8) or with GLP-2 treatment (3.5 μg/kg body wt per h, SBS+GLP-2, n = 7) and compared with a group of unresected preterm pigs (control, n = 5). After 5 days of TPN, all piglets were fed enterally for 24 h, and a nutrient balance study was performed. Intestinal resection was associated with markedly reduced endogenous GLP-2 levels. GLP-2 increased the relative absorption of wet weight (46 vs. 22%), energy (79 vs. 64%), and all macronutrients (all parameters P <0.05). These findings were supported by a 200% increase in sucrase and maltase activities, a 50% increase in small intestinal epithelial volume (P <0.05), as well as increased DNA and protein contents and increased total protein synthesis rate in SBS+GLP-2 vs. SBS pigs (+100%, P <0.05). Following intestinal resection in preterm pigs, GLP-2 induced structural and functional adaptation, resulting in a higher relative absorption of fluid and macronutrients. GLP-2 treatment may be a promising therapy to enhance intestinal adaptation and improve digestive function in preterm infants with jejunostomy following intestinal resection.

    U2 - 10.1152/ajpgi.00064.2013

    DO - 10.1152/ajpgi.00064.2013

    M3 - Journal article

    C2 - 23764891

    VL - 305

    JO - A J P: Gastrointestinal and Liver Physiology (Online)

    JF - A J P: Gastrointestinal and Liver Physiology (Online)

    SN - 1522-1547

    M1 - G277-G285

    ER -