Glioblastomas with primitive neuronal component harbor a distinct methylation and copy-number profile with inactivation of TP53, PTEN, and RB1

Abigail K. Suwala, Damian Stichel, Daniel Schrimpf, Sybren L.N. Maas, Martin Sill, Hildegard Dohmen, Rouzbeh Banan, Annekathrin Reinhardt, Philipp Sievers, Felix Hinz, Mirjam Blattner-Johnson, Christian Hartmann, Leonille Schweizer, Henning B. Boldt, Bjarne Winther Kristensen, Jens Schittenhelm, Matthew D. Wood, Guillaume Chotard, Rolf Bjergvig, Anirban DasUri Tabori, Martin Hasselblatt, Andrey Korshunov, Zied Abdullaev, Martha Quezado, Kenneth Aldape, Patrick N. Harter, Matija Snuderl, Jürgen Hench, Stephan Frank, Till Acker, Sebastian Brandner, Frank Winkler, Pieter Wesseling, Stefan M. Pfister, David E. Reuss, Wolfgang Wick, Andreas von Deimling, David T.W. Jones, Felix Sahm

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

11 Downloads (Pure)

Abstrakt

Glioblastoma IDH-wildtype presents with a wide histological spectrum. Some features are so distinctive that they are considered as separate histological variants or patterns for the purpose of classification. However, these usually lack defined (epi-)genetic alterations or profiles correlating with this histology. Here, we describe a molecular subtype with overlap to the unique histological pattern of glioblastoma with primitive neuronal component. Our cohort consists of 63 IDH-wildtype glioblastomas that harbor a characteristic DNA methylation profile. Median age at diagnosis was 59.5 years. Copy-number variations and genetic sequencing revealed frequent alterations in TP53, RB1 and PTEN, with fewer gains of chromosome 7 and homozygous CDKN2A/B deletions than usually described for IDH-wildtype glioblastoma. Gains of chromosome 1 were detected in more than half of the cases. A poorly differentiated phenotype with frequent absence of GFAP expression, high proliferation index and strong staining for p53 and TTF1 often caused misleading histological classification as carcinoma metastasis or primitive neuroectodermal tumor. Clinically, many patients presented with leptomeningeal dissemination and spinal metastasis. Outcome was poor with a median overall survival of only 12 months. Overall, we describe a new molecular subtype of IDH-wildtype glioblastoma with a distinct histological appearance and genetic signature.

OriginalsprogEngelsk
TidsskriftActa Neuropathologica
Vol/bind142
Udgave nummer1
Sider (fra-til)179-189
ISSN0001-6322
DOI
StatusUdgivet - 1. jul. 2021

Bibliografisk note

Funding Information:
The work has received funding from the European Unions Horizon 2020 Research and Innovation Programme under Grant Agreement No 761072 (DACOMAT).

Fingeraftryk

Dyk ned i forskningsemnerne om 'Glioblastomas with primitive neuronal component harbor a distinct methylation and copy-number profile with inactivation of TP53, PTEN, and RB1'. Sammen danner de et unikt fingeraftryk.

Citationsformater