Giant Unilamellar Vesicle Electroformation: From Lipid Mixtures to Native Membranes Under Physiological Conditions

Philippe Meleard, Luis Bagatolli, Tanja Pott

Publikation: Bidrag til tidsskriftReviewForskning

Resumé

Udgivelsesdato: november
OriginalsprogEngelsk
BogserieMethods in Enzymology
Vol/bind465
Sider (fra-til)161-176
Antal sider16
ISSN0076-6879
StatusUdgivet - 1. nov. 2009

Fingeraftryk

Unilamellar Liposomes
Membranes
Lipids
Erythrocyte Membrane
Lipid Bilayers
Cell membranes
Electrolytes
Optical microscopy
Microscopy
Buffers
Membrane Proteins
Blood
Cells
Cell Membrane

Citer dette

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title = "Giant Unilamellar Vesicle Electroformation: From Lipid Mixtures to Native Membranes Under Physiological Conditions",
abstract = "Giant unilamellar vesicles (GUVs) are well-known model systems, especially because they are easily observable using optical microscopy. In this chapter, we revisit in detail the versatile GUV electroformation protocol. We demonstrate how GUV electroformation can be adapted to various membrane systems including synthetic lipid mixtures, natural lipid extracts, and bilayers containing membrane proteins. Further, we show how to adjust this protocol to a given aqueous environment and prove that GUVs can be obtained under physiologically relevant conditions, that is, in the presence of electrolytes. Finally, we provide firm evidence that electroformation is a method of choice to produce giant vesicles from native cell membranes. This is illustrated with the example of GUV electroformation from red blood cell ghosts in a physiologically pertinent buffer. GUVs obtained in this manner maintain the native membrane asymmetry, thereby validating the physiological relevance of GUV electroformation.",
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Giant Unilamellar Vesicle Electroformation: From Lipid Mixtures to Native Membranes Under Physiological Conditions. / Meleard, Philippe; Bagatolli, Luis; Pott, Tanja.

I: Methods in Enzymology, Bind 465, 01.11.2009, s. 161-176.

Publikation: Bidrag til tidsskriftReviewForskning

TY - JOUR

T1 - Giant Unilamellar Vesicle Electroformation: From Lipid Mixtures to Native Membranes Under Physiological Conditions

AU - Meleard, Philippe

AU - Bagatolli, Luis

AU - Pott, Tanja

PY - 2009/11/1

Y1 - 2009/11/1

N2 - Giant unilamellar vesicles (GUVs) are well-known model systems, especially because they are easily observable using optical microscopy. In this chapter, we revisit in detail the versatile GUV electroformation protocol. We demonstrate how GUV electroformation can be adapted to various membrane systems including synthetic lipid mixtures, natural lipid extracts, and bilayers containing membrane proteins. Further, we show how to adjust this protocol to a given aqueous environment and prove that GUVs can be obtained under physiologically relevant conditions, that is, in the presence of electrolytes. Finally, we provide firm evidence that electroformation is a method of choice to produce giant vesicles from native cell membranes. This is illustrated with the example of GUV electroformation from red blood cell ghosts in a physiologically pertinent buffer. GUVs obtained in this manner maintain the native membrane asymmetry, thereby validating the physiological relevance of GUV electroformation.

AB - Giant unilamellar vesicles (GUVs) are well-known model systems, especially because they are easily observable using optical microscopy. In this chapter, we revisit in detail the versatile GUV electroformation protocol. We demonstrate how GUV electroformation can be adapted to various membrane systems including synthetic lipid mixtures, natural lipid extracts, and bilayers containing membrane proteins. Further, we show how to adjust this protocol to a given aqueous environment and prove that GUVs can be obtained under physiologically relevant conditions, that is, in the presence of electrolytes. Finally, we provide firm evidence that electroformation is a method of choice to produce giant vesicles from native cell membranes. This is illustrated with the example of GUV electroformation from red blood cell ghosts in a physiologically pertinent buffer. GUVs obtained in this manner maintain the native membrane asymmetry, thereby validating the physiological relevance of GUV electroformation.

M3 - Review

VL - 465

SP - 161

EP - 176

JO - Methods in Enzymology

JF - Methods in Enzymology

SN - 0076-6879

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