Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Morad Kamand; Mirolyuba Ilieva; Sheena Louise Forsberg; Mads Thomassen; Åsa Fex Svenningsen; Bjørn Holst; Morten Meyer; Tanja Maria Michel

doi:10.1016/j.scr.2020.101834

Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Morad Kamand, Mirolyuba Ilieva, Sheena Louise Forsberg, Mads Thomassen, Åsa Fex Svenningsen, Bjørn Holst, Morten Meyer, Tanja Maria Michel^*

^*Kontaktforfatter

Bioneer A/S

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

113 Downloads (Pure)

Abstract

Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.

Originalsprog	Engelsk
Artikelnummer	101834
Tidsskrift	Stem Cell Research
Vol/bind	46
Antal sider	4
ISSN	1873-5061
DOI	https://doi.org/10.1016/j.scr.2020.101834
Status	Udgivet - jul. 2020

Dokumenter og links

10.1016/j.scr.2020.101834Licens: CC BY-NC-ND

Open Access VersionForlagets udgivne version, 1,09 MBLicens: CC BY-NC-ND

1 Ph.d.-afhandling

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain
Kamand, M., 24. jun. 2022, Syddansk Universitet. Det Sundhedsvidenskabelige Fakultet. 225 s.
Publikation: Afhandling › Ph.d.-afhandling

Citationsformater

@article{b1651e5f246c4fc2b37174cb64310ea9,

title = "Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder",

abstract = "Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.",

author = "Morad Kamand and Mirolyuba Ilieva and Forsberg, {Sheena Louise} and Mads Thomassen and {Fex Svenningsen}, {\AA}sa and Bj{\o}rn Holst and Morten Meyer and Michel, {Tanja Maria}",

year = "2020",

month = jul,

doi = "10.1016/j.scr.2020.101834",

language = "English",

volume = "46",

journal = "Stem Cell Research",

issn = "1873-5061",

publisher = "Elsevier",

}

TY - JOUR

T1 - Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

AU - Kamand, Morad

AU - Ilieva, Mirolyuba

AU - Forsberg, Sheena Louise

AU - Thomassen, Mads

AU - Fex Svenningsen, Åsa

AU - Holst, Bjørn

AU - Meyer, Morten

AU - Michel, Tanja Maria

PY - 2020/7

Y1 - 2020/7

N2 - Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.

AB - Autism spectrum disorders are characterized by impaired social interaction and communication as well as restricted and repetitive interests and behavior. Increasing evidence points to an early-stage disruption of brain development. A human-induced pluripotent stem cell line (SDUKIi002-A) was created from skin fibroblasts from a 22-year old autistic male identified in the “FYNEN-cohort” of Southern Denmark. Reprogramming of the fibroblasts was performed using integration-free episomal plasmids. Further characterization confirmed the expression of pluripotency markers, differentiation into the three germ layers, absence of chromosomal abnormalities, and mycoplasma infection.

U2 - 10.1016/j.scr.2020.101834

DO - 10.1016/j.scr.2020.101834

M3 - Journal article

C2 - 32447258

AN - SCOPUS:85084788952

SN - 1873-5061

VL - 46

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 101834

ER -

Generation of human induced pluripotent stem cells (SDUKIi002-A) from a 22-year-old male diagnosed with autism spectrum disorder

Abstract

Dokumenter og links

Fingeraftryk

Relaterede publikationer

Studying autism spectrum disorders (ASD) using a three-dimensional brain organoid model derived from patient-specific induced pluripotent stem cells (iPSCs): Autism from skin to brain

Citationsformater