Generation and characterization of induced pluripotent stem (iPS) cell line (SDUCTi001-A) using fibroblasts derived from male-healthy donor

Morad Kamand; Sonja Hohmann; Sheena Louise Forsberg; Mads Thomassen; Mirolyuba Ilieva; Åsa Fex Svenningsen; Morten Meyer; Tanja Maria Michel

doi:10.1016/j.scr.2022.102961

Generation and characterization of induced pluripotent stem (iPS) cell line (SDUCTi001-A) using fibroblasts derived from male-healthy donor

Morad Kamand^*, Sonja Hohmann, Sheena Louise Forsberg, Mads Thomassen, Mirolyuba Ilieva, Åsa Fex Svenningsen, Morten Meyer, Tanja Maria Michel

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Abstrakt

Induced pluripotent stem (iPS) cell lines have wide valuable applications in experimental research, including developmental, pathological, and drug screening studies. Using integration-free episomal plasmids, we have generated a new iPS cell line from a 26-year-old healthy male donor. Characterization of the established cell line confirmed the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

Originalsprog	Engelsk
Artikelnummer	102961
Tidsskrift	Stem Cell Research
Vol/bind	65
ISSN	1873-5061
DOI	https://doi.org/10.1016/j.scr.2022.102961
Status	Udgivet - dec. 2022

Bibliografisk note

Funding Information:
We would like to acknowledge Associate Professor Tore Bjerregaard Stage for providing RNA from TMOi001-A cells, and the Psychiatric Research Fund of the Region of Southern Denmark (Psykiatriens Forskningsfond; R36-A1784/1808 1 0861), Faculty of Health Sciences, University of Southern Denmark (SDUSF‐2016‐144 ‐(738)) Denmark, and the JASCHA Foundation, Denmark for financial support (6208/1808 2 0861).

Dokumenter og links

10.1016/j.scr.2022.102961Licens: CC BY-NC-ND

Open Access VersionForlagets udgivne version, 2 MBLicens: CC BY-NC-ND

Citationsformater

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abstract = "Induced pluripotent stem (iPS) cell lines have wide valuable applications in experimental research, including developmental, pathological, and drug screening studies. Using integration-free episomal plasmids, we have generated a new iPS cell line from a 26-year-old healthy male donor. Characterization of the established cell line confirmed the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.",

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AU - Kamand, Morad

AU - Hohmann, Sonja

AU - Louise Forsberg, Sheena

AU - Thomassen, Mads

AU - Ilieva, Mirolyuba

AU - Fex Svenningsen, Åsa

AU - Meyer, Morten

AU - Maria Michel, Tanja

PY - 2022/12

Y1 - 2022/12

N2 - Induced pluripotent stem (iPS) cell lines have wide valuable applications in experimental research, including developmental, pathological, and drug screening studies. Using integration-free episomal plasmids, we have generated a new iPS cell line from a 26-year-old healthy male donor. Characterization of the established cell line confirmed the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

AB - Induced pluripotent stem (iPS) cell lines have wide valuable applications in experimental research, including developmental, pathological, and drug screening studies. Using integration-free episomal plasmids, we have generated a new iPS cell line from a 26-year-old healthy male donor. Characterization of the established cell line confirmed the expression of pluripotency markers, differentiation into the three germ layers, and absence of chromosomal abnormalities.

U2 - 10.1016/j.scr.2022.102961

DO - 10.1016/j.scr.2022.102961

M3 - Journal article

C2 - 36402078

AN - SCOPUS:85141998328

VL - 65

JO - Stem Cell Research

JF - Stem Cell Research

SN - 1873-5061

M1 - 102961

ER -

Generation and characterization of induced pluripotent stem (iPS) cell line (SDUCTi001-A) using fibroblasts derived from male-healthy donor

Abstrakt

Bibliografisk note

Dokumenter og links

Fingeraftryk

Citationsformater