Fibulin-1C, C1 Esterase Inhibitor and Glucose Regulated Protein 75 Interact with the CREC Proteins, Calumenin and Reticulocalbin

G. A. W. Hansen, M. Ludvigsen, C. Jacobsen, C. Cangemi, L. M. Rasmussen, H. Vorum, B. Honore

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

Affinity purification, immunoprecipitation, gel electrophoresis and mass spectrometry were used to identify fibulin-1C, C1 esterase inhibitor and glucose regulated protein 75, grp75, as binding partners of the CREC proteins, calumenin and reticulocalbin. Surface plasmon resonance was used to verify the interaction of all three proteins with each of the CREC proteins. Fibulin-1C interacts with calumenin and reticulocalbin with an estimated dissociation constant around 50-60 nM. The interaction, at least for reticulocalbin, was not dependent upon the presence of Ca 2+. C1 esterase inhibitor interacted with both proteins with an estimated dissociation constant at 1 μM for reticulocalbin and 150 nM for calumenin. The interaction, at least for calumenin, was dependent upon the presence of Ca 2+ with strong interaction at 3.5 mM while no detectable interaction could be found at 0.1 mM. Grp75 binds with an affinity of approximately 3-7 nM with reticulocalbin as well as with calumenin. These interactions suggest functional participation of the CREC proteins in chaperone activity, cell proliferation and transformation, cellular aging, haemostasis and thrombosis as well as modulation of the complement system in fighting bacterial infection.

OriginalsprogEngelsk
Artikelnummer e0132283
TidsskriftP L o S One
Vol/bind10
Udgave nummer7
ISSN1932-6203
DOI
StatusUdgivet - 10. jul. 2015

Bibliografisk note

Danish Medical Research Council [52-00-1004, 22-01-0249]; Novo Nordic Foundation; Danish Cancer Foundation [DS 02 071]; Danish Heart Foundation [08-4-R64-A2008-B894-22450] 1 e0132283 26161649

Emneord

  • CALCIUM-BINDING PROTEIN MULTIPLE EF-HAND ENDOPLASMIC-RETICULUM CA2+-BINDING PROTEIN EXTRACELLULAR CALUMENIN PROTEOMIC APPROACH SECRETORY PATHWAY POTENTIAL ROLE CANCER CELLS EXPRESSION

Fingeraftryk Dyk ned i forskningsemnerne om 'Fibulin-1C, C1 Esterase Inhibitor and Glucose Regulated Protein 75 Interact with the CREC Proteins, Calumenin and Reticulocalbin'. Sammen danner de et unikt fingeraftryk.

Citationsformater