FDOPA metabolism in the adult porcine brain: influence of tracer circulation time and VOI selection on estimates of striatal DOPA decarboxylation.

E H Danielsen, D F Smith, Flemming Andersen, A D Gee, D Bender, Søren Baarsgaard Hansen, F Hermansen, L Østergaard, P Cumming, A Gjedde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2001-Oct-30
OriginalsprogEngelsk
TidsskriftJournal of Neuroscience Methods
Vol/bind111
Udgave nummer2
Sider (fra-til)157-68
Antal sider12
ISSN0165-0270
DOI
StatusUdgivet - 2001
Udgivet eksterntJa

Fingeraftryk

Decarboxylation
Radioactivity

Citer dette

Danielsen, E H ; Smith, D F ; Andersen, Flemming ; Gee, A D ; Bender, D ; Hansen, Søren Baarsgaard ; Hermansen, F ; Østergaard, L ; Cumming, P ; Gjedde, A. / FDOPA metabolism in the adult porcine brain: influence of tracer circulation time and VOI selection on estimates of striatal DOPA decarboxylation. I: Journal of Neuroscience Methods. 2001 ; Bind 111, Nr. 2. s. 157-68.
@article{b95a9d393cc34de7ade6ab760ceb68c9,
title = "FDOPA metabolism in the adult porcine brain: influence of tracer circulation time and VOI selection on estimates of striatal DOPA decarboxylation.",
abstract = "Different methodologies for PET data analysis influence the magnitude of estimates of blood-brain transfer coefficients and rate constants for the metabolism of FDOPA in living striatum. We now test the effects on several kinetic parameters of automatic procedures for volume of interest (VOI) selection. We also tested the sensitivity of the estimates to dynamic frame sequence duration, and produced a standard method for minimizing the variations in physiological estimates for FDOPA kinetics in minipig brain. We used minipigs because our previous work has shown them to provide an appropriate animal model for study normal and pathological cerebral DOPA metabolism using PET. Time-activity curves in striatum of adult minipigs were acquired in VOIs defined manually on MR-images, or alternatively on the basis of the radioactivity concentration based on the most radioactive voxel in the last scan frame. For all frame sequences, the relative decarboxylase activity (k(3)(D)) declined significantly (P < 0.006) as the VOI threshold declined from 95 to 70{\%} of the most radioactive voxel. Irrespective of VOI size, the magnitude of k(3)(D) declined significantly (P < 0.001) from 0.074+/-0.008 to 0.045+/-0.005 per min (mean+/-S.E.M.) as total sequence length increased from 60 to 120 min circulation. The method of VOI selection had no significant effect on the striatum decarboxylation index of FDOPA calculated relative to the radioactivity in cerebellum (k(3)(S)).",
author = "Danielsen, {E H} and Smith, {D F} and Flemming Andersen and Gee, {A D} and D Bender and Hansen, {S{\o}ren Baarsgaard} and F Hermansen and L {\O}stergaard and P Cumming and A Gjedde",
year = "2001",
doi = "10.1016/S0165-0270(01)00453-8",
language = "English",
volume = "111",
pages = "157--68",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "2",

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FDOPA metabolism in the adult porcine brain: influence of tracer circulation time and VOI selection on estimates of striatal DOPA decarboxylation. / Danielsen, E H; Smith, D F; Andersen, Flemming; Gee, A D; Bender, D; Hansen, Søren Baarsgaard; Hermansen, F; Østergaard, L; Cumming, P; Gjedde, A.

I: Journal of Neuroscience Methods, Bind 111, Nr. 2, 2001, s. 157-68.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - FDOPA metabolism in the adult porcine brain: influence of tracer circulation time and VOI selection on estimates of striatal DOPA decarboxylation.

AU - Danielsen, E H

AU - Smith, D F

AU - Andersen, Flemming

AU - Gee, A D

AU - Bender, D

AU - Hansen, Søren Baarsgaard

AU - Hermansen, F

AU - Østergaard, L

AU - Cumming, P

AU - Gjedde, A

PY - 2001

Y1 - 2001

N2 - Different methodologies for PET data analysis influence the magnitude of estimates of blood-brain transfer coefficients and rate constants for the metabolism of FDOPA in living striatum. We now test the effects on several kinetic parameters of automatic procedures for volume of interest (VOI) selection. We also tested the sensitivity of the estimates to dynamic frame sequence duration, and produced a standard method for minimizing the variations in physiological estimates for FDOPA kinetics in minipig brain. We used minipigs because our previous work has shown them to provide an appropriate animal model for study normal and pathological cerebral DOPA metabolism using PET. Time-activity curves in striatum of adult minipigs were acquired in VOIs defined manually on MR-images, or alternatively on the basis of the radioactivity concentration based on the most radioactive voxel in the last scan frame. For all frame sequences, the relative decarboxylase activity (k(3)(D)) declined significantly (P < 0.006) as the VOI threshold declined from 95 to 70% of the most radioactive voxel. Irrespective of VOI size, the magnitude of k(3)(D) declined significantly (P < 0.001) from 0.074+/-0.008 to 0.045+/-0.005 per min (mean+/-S.E.M.) as total sequence length increased from 60 to 120 min circulation. The method of VOI selection had no significant effect on the striatum decarboxylation index of FDOPA calculated relative to the radioactivity in cerebellum (k(3)(S)).

AB - Different methodologies for PET data analysis influence the magnitude of estimates of blood-brain transfer coefficients and rate constants for the metabolism of FDOPA in living striatum. We now test the effects on several kinetic parameters of automatic procedures for volume of interest (VOI) selection. We also tested the sensitivity of the estimates to dynamic frame sequence duration, and produced a standard method for minimizing the variations in physiological estimates for FDOPA kinetics in minipig brain. We used minipigs because our previous work has shown them to provide an appropriate animal model for study normal and pathological cerebral DOPA metabolism using PET. Time-activity curves in striatum of adult minipigs were acquired in VOIs defined manually on MR-images, or alternatively on the basis of the radioactivity concentration based on the most radioactive voxel in the last scan frame. For all frame sequences, the relative decarboxylase activity (k(3)(D)) declined significantly (P < 0.006) as the VOI threshold declined from 95 to 70% of the most radioactive voxel. Irrespective of VOI size, the magnitude of k(3)(D) declined significantly (P < 0.001) from 0.074+/-0.008 to 0.045+/-0.005 per min (mean+/-S.E.M.) as total sequence length increased from 60 to 120 min circulation. The method of VOI selection had no significant effect on the striatum decarboxylation index of FDOPA calculated relative to the radioactivity in cerebellum (k(3)(S)).

U2 - 10.1016/S0165-0270(01)00453-8

DO - 10.1016/S0165-0270(01)00453-8

M3 - Journal article

C2 - 11595282

VL - 111

SP - 157

EP - 168

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 2

ER -