False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

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Resumé

Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

OriginalsprogEngelsk
TidsskriftThe Open Microbiology Journal
Vol/bind13
Sider (fra-til)51-54
ISSN1874-2858
DOI
StatusUdgivet - apr. 2019

Fingeraftryk

Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Insertional Mutagenesis
Retrospective Studies

Citer dette

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title = "False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients",
abstract = "Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3{\%} of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.",
keywords = "Bordetella pertussis, False-positive, IS1001, IS1002, IS481, Real-time PCR",
author = "H{\o}gh, {Silje Vermedal} and Agergaard, {Charlotte Nielsen} and Skov, {Marianne Nielsine} and Michael Kemp",
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month = "4",
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language = "English",
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pages = "51--54",
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False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients. / Høgh, Silje Vermedal; Agergaard, Charlotte Nielsen; Skov, Marianne Nielsine; Kemp, Michael.

I: The Open Microbiology Journal, Bind 13, 04.2019, s. 51-54.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

AU - Høgh, Silje Vermedal

AU - Agergaard, Charlotte Nielsen

AU - Skov, Marianne Nielsine

AU - Kemp, Michael

PY - 2019/4

Y1 - 2019/4

N2 - Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

AB - Background: Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis. Objective: The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR. Methods: Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001. Results: The results showed that 2.3% of the samples were false-positive for Bordetella pertussis. Conclusion: In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

KW - Bordetella pertussis

KW - False-positive

KW - IS1001

KW - IS1002

KW - IS481

KW - Real-time PCR

U2 - 10.2174/1874285801913010051

DO - 10.2174/1874285801913010051

M3 - Journal article

VL - 13

SP - 51

EP - 54

JO - The Open Microbiology Journal

JF - The Open Microbiology Journal

SN - 1874-2858

ER -