Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

Yangxin Wang; Ningning Zhang; Deming Tan; Zhenhui Qi; Changzhu Wu

doi:10.3389/fbioe.2020.00714

Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

Yangxin Wang, Ningning Zhang, Deming Tan, Zhenhui Qi, Changzhu Wu^*

^*Kontaktforfatter

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO₃)₂⋅6H₂O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

Originalsprog	Engelsk
Artikelnummer	714
Tidsskrift	Frontiers in Bioengineering and Biotechnology
Vol/bind	8
ISSN	2296-4185
DOI	https://doi.org/10.3389/fbioe.2020.00714
Status	Udgivet - 7. jul. 2020

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10.3389/fbioe.2020.00714Licens: CC BY

Open Access VersionForlagets udgivne version, 1,21 MBLicens: CC BY

Citationsformater

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title = "Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent",

abstract = "In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.",

keywords = "biocatalysis, enzymatic reactions, heterogeneous catalysis, metal–organic frameworks, size-selective catalysis",

author = "Yangxin Wang and Ningning Zhang and Deming Tan and Zhenhui Qi and Changzhu Wu",

year = "2020",

month = jul,

day = "7",

doi = "10.3389/fbioe.2020.00714",

language = "English",

volume = "8",

journal = "Frontiers in Bioengineering and Biotechnology",

issn = "2296-4185",

publisher = "Frontiers Media S.A.",

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TY - JOUR

T1 - Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

AU - Wang, Yangxin

AU - Zhang, Ningning

AU - Tan, Deming

AU - Qi, Zhenhui

AU - Wu, Changzhu

PY - 2020/7/7

Y1 - 2020/7/7

N2 - In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

AB - In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO3)2⋅6H2O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

KW - biocatalysis

KW - enzymatic reactions

KW - heterogeneous catalysis

KW - metal–organic frameworks

KW - size-selective catalysis

U2 - 10.3389/fbioe.2020.00714

DO - 10.3389/fbioe.2020.00714

M3 - Journal article

C2 - 32733866

AN - SCOPUS:85088468868

SN - 2296-4185

VL - 8

JO - Frontiers in Bioengineering and Biotechnology

JF - Frontiers in Bioengineering and Biotechnology

M1 - 714

ER -

Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

Abstract

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