Exposure to silver nanoparticles induces size- and dose-dependent oxidative stress and cytotoxicity in human colon carcinoma cells

Rona Miethling-Graff, Rita Rumpker, Madeleine Richter, Thiago Verano-Braga, Frank Kjeldsen, Jonathan R. Brewer, James Hoyland, Horst-Günter Rubahn, Helmut Erdmann

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The antimicrobial properties of silver nanoparticles (AgNPs) have made these particles one of the most frequently utilized nanomaterials in consumer products; therefore, a comprehensive understanding of their toxicity is necessary. In particular, information about the cellular uptake and size dependence of AgNPs is insufficient. In this study, we evaluated the size-dependent effects of AgNPs by treating the human LoVo cell line, an intestinal epithelium model, with spherical AgNPs of well-defined sizes (10, 20, 40, 60 and 100nm). The cellular uptake was visualized by confocal laser scanning microscopy, and various cytotoxicity parameters were analyzed in a size- and dose-dependent manner. In addition, the cellular proteomic response to 20 and 100nm AgNPs was investigated to increase the understanding of potential mechanisms of action. Our data indicated that cellular uptake and toxicity were regulated by size; smaller particles easily penetrated the cells, and 100nm particles did not. It was hypothesized that this size-dependent effect resulted from the stimulation of a signaling cascade that generated ROS and inflammatory markers, leading to mitochondrial dysfunction and subsequently inducing apoptosis. By contrast, the cell proliferation, was independent of AgNPs particle size, indicating a differentially regulated, ROS-independent pathway.

OriginalsprogEngelsk
TidsskriftToxicology in Vitro
Vol/bind28
Udgave nummer7
Sider (fra-til)1280–1289
ISSN0887-2333
DOI
StatusUdgivet - 2014

Fingeraftryk

Oxidative stress
Intestinal Mucosa
Cytotoxicity
Silver
Particle Size
Confocal Microscopy
Action Potentials
Toxicity
Colon
Cells
Cell Proliferation
Apoptosis
Nanoparticles
Cell Line
Consumer products
Cell proliferation
Nanostructured materials
Microscopic examination
Particle size
Scanning

Citer dette

Miethling-Graff, Rona ; Rumpker, Rita ; Richter, Madeleine ; Verano-Braga, Thiago ; Kjeldsen, Frank ; Brewer, Jonathan R. ; Hoyland, James ; Rubahn, Horst-Günter ; Erdmann, Helmut. / Exposure to silver nanoparticles induces size- and dose-dependent oxidative stress and cytotoxicity in human colon carcinoma cells. I: Toxicology in Vitro. 2014 ; Bind 28, Nr. 7. s. 1280–1289.
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title = "Exposure to silver nanoparticles induces size- and dose-dependent oxidative stress and cytotoxicity in human colon carcinoma cells",
abstract = "The antimicrobial properties of silver nanoparticles (AgNPs) have made these particles one of the most frequently utilized nanomaterials in consumer products; therefore, a comprehensive understanding of their toxicity is necessary. In particular, information about the cellular uptake and size dependence of AgNPs is insufficient. In this study, we evaluated the size-dependent effects of AgNPs by treating the human LoVo cell line, an intestinal epithelium model, with spherical AgNPs of well-defined sizes (10, 20, 40, 60 and 100nm). The cellular uptake was visualized by confocal laser scanning microscopy, and various cytotoxicity parameters were analyzed in a size- and dose-dependent manner. In addition, the cellular proteomic response to 20 and 100nm AgNPs was investigated to increase the understanding of potential mechanisms of action. Our data indicated that cellular uptake and toxicity were regulated by size; smaller particles easily penetrated the cells, and 100nm particles did not. It was hypothesized that this size-dependent effect resulted from the stimulation of a signaling cascade that generated ROS and inflammatory markers, leading to mitochondrial dysfunction and subsequently inducing apoptosis. By contrast, the cell proliferation, was independent of AgNPs particle size, indicating a differentially regulated, ROS-independent pathway.",
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Exposure to silver nanoparticles induces size- and dose-dependent oxidative stress and cytotoxicity in human colon carcinoma cells. / Miethling-Graff, Rona; Rumpker, Rita; Richter, Madeleine; Verano-Braga, Thiago; Kjeldsen, Frank; Brewer, Jonathan R.; Hoyland, James; Rubahn, Horst-Günter; Erdmann, Helmut.

I: Toxicology in Vitro, Bind 28, Nr. 7, 2014, s. 1280–1289.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Exposure to silver nanoparticles induces size- and dose-dependent oxidative stress and cytotoxicity in human colon carcinoma cells

AU - Miethling-Graff, Rona

AU - Rumpker, Rita

AU - Richter, Madeleine

AU - Verano-Braga, Thiago

AU - Kjeldsen, Frank

AU - Brewer, Jonathan R.

AU - Hoyland, James

AU - Rubahn, Horst-Günter

AU - Erdmann, Helmut

N1 - Copyright © 2014 Elsevier Ltd. All rights reserved.

PY - 2014

Y1 - 2014

N2 - The antimicrobial properties of silver nanoparticles (AgNPs) have made these particles one of the most frequently utilized nanomaterials in consumer products; therefore, a comprehensive understanding of their toxicity is necessary. In particular, information about the cellular uptake and size dependence of AgNPs is insufficient. In this study, we evaluated the size-dependent effects of AgNPs by treating the human LoVo cell line, an intestinal epithelium model, with spherical AgNPs of well-defined sizes (10, 20, 40, 60 and 100nm). The cellular uptake was visualized by confocal laser scanning microscopy, and various cytotoxicity parameters were analyzed in a size- and dose-dependent manner. In addition, the cellular proteomic response to 20 and 100nm AgNPs was investigated to increase the understanding of potential mechanisms of action. Our data indicated that cellular uptake and toxicity were regulated by size; smaller particles easily penetrated the cells, and 100nm particles did not. It was hypothesized that this size-dependent effect resulted from the stimulation of a signaling cascade that generated ROS and inflammatory markers, leading to mitochondrial dysfunction and subsequently inducing apoptosis. By contrast, the cell proliferation, was independent of AgNPs particle size, indicating a differentially regulated, ROS-independent pathway.

AB - The antimicrobial properties of silver nanoparticles (AgNPs) have made these particles one of the most frequently utilized nanomaterials in consumer products; therefore, a comprehensive understanding of their toxicity is necessary. In particular, information about the cellular uptake and size dependence of AgNPs is insufficient. In this study, we evaluated the size-dependent effects of AgNPs by treating the human LoVo cell line, an intestinal epithelium model, with spherical AgNPs of well-defined sizes (10, 20, 40, 60 and 100nm). The cellular uptake was visualized by confocal laser scanning microscopy, and various cytotoxicity parameters were analyzed in a size- and dose-dependent manner. In addition, the cellular proteomic response to 20 and 100nm AgNPs was investigated to increase the understanding of potential mechanisms of action. Our data indicated that cellular uptake and toxicity were regulated by size; smaller particles easily penetrated the cells, and 100nm particles did not. It was hypothesized that this size-dependent effect resulted from the stimulation of a signaling cascade that generated ROS and inflammatory markers, leading to mitochondrial dysfunction and subsequently inducing apoptosis. By contrast, the cell proliferation, was independent of AgNPs particle size, indicating a differentially regulated, ROS-independent pathway.

U2 - 10.1016/j.tiv.2014.06.005

DO - 10.1016/j.tiv.2014.06.005

M3 - Journal article

VL - 28

SP - 1280

EP - 1289

JO - Toxicology in Vitro

JF - Toxicology in Vitro

SN - 0887-2333

IS - 7

ER -