Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase

Ana Rioz-Martínez, Malgorzata Kopacz, Gonzalo de Gonzalo, Daniel E Torres Pazmiño, Vicente Gotor, Marco W Fraaije

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

A bacterial flavin-containing monooxygenase (FMO), fused to phosphite dehydrogenase, has been used to explore its biocatalytic potential. The bifunctional biocatalyst could be expressed in high amounts in Escherichia coli and was able to oxidize indole and indole derivatives into a variety of indigo compounds. The monooxygenase also performs the sulfoxidation of a wide range of prochiral sulfides, showing moderate to good enantioselectivities in forming chiral sulfoxides.

OriginalsprogEngelsk
TidsskriftOrganic & Biomolecular Chemistry
Vol/bind9
Udgave nummer5
Sider (fra-til)1337-41
Antal sider5
ISSN1477-0520
DOI
StatusUdgivet - 7. mar. 2011
Udgivet eksterntJa

Fingeraftryk

dimethylaniline monooxygenase (N-oxide forming)
Indigo Carmine
Sulfoxides
Enantioselectivity
Sulfides
Mixed Function Oxygenases
Escherichia coli
Derivatives
Enzymes
indole

Citer dette

Rioz-Martínez, A., Kopacz, M., de Gonzalo, G., Torres Pazmiño, D. E., Gotor, V., & Fraaije, M. W. (2011). Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase. Organic & Biomolecular Chemistry, 9(5), 1337-41. https://doi.org/10.1039/c0ob00988a
Rioz-Martínez, Ana ; Kopacz, Malgorzata ; de Gonzalo, Gonzalo ; Torres Pazmiño, Daniel E ; Gotor, Vicente ; Fraaije, Marco W. / Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase. I: Organic & Biomolecular Chemistry. 2011 ; Bind 9, Nr. 5. s. 1337-41.
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abstract = "A bacterial flavin-containing monooxygenase (FMO), fused to phosphite dehydrogenase, has been used to explore its biocatalytic potential. The bifunctional biocatalyst could be expressed in high amounts in Escherichia coli and was able to oxidize indole and indole derivatives into a variety of indigo compounds. The monooxygenase also performs the sulfoxidation of a wide range of prochiral sulfides, showing moderate to good enantioselectivities in forming chiral sulfoxides.",
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Rioz-Martínez, A, Kopacz, M, de Gonzalo, G, Torres Pazmiño, DE, Gotor, V & Fraaije, MW 2011, 'Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase', Organic & Biomolecular Chemistry, bind 9, nr. 5, s. 1337-41. https://doi.org/10.1039/c0ob00988a

Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase. / Rioz-Martínez, Ana; Kopacz, Malgorzata; de Gonzalo, Gonzalo; Torres Pazmiño, Daniel E; Gotor, Vicente; Fraaije, Marco W.

I: Organic & Biomolecular Chemistry, Bind 9, Nr. 5, 07.03.2011, s. 1337-41.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase

AU - Rioz-Martínez, Ana

AU - Kopacz, Malgorzata

AU - de Gonzalo, Gonzalo

AU - Torres Pazmiño, Daniel E

AU - Gotor, Vicente

AU - Fraaije, Marco W

PY - 2011/3/7

Y1 - 2011/3/7

N2 - A bacterial flavin-containing monooxygenase (FMO), fused to phosphite dehydrogenase, has been used to explore its biocatalytic potential. The bifunctional biocatalyst could be expressed in high amounts in Escherichia coli and was able to oxidize indole and indole derivatives into a variety of indigo compounds. The monooxygenase also performs the sulfoxidation of a wide range of prochiral sulfides, showing moderate to good enantioselectivities in forming chiral sulfoxides.

AB - A bacterial flavin-containing monooxygenase (FMO), fused to phosphite dehydrogenase, has been used to explore its biocatalytic potential. The bifunctional biocatalyst could be expressed in high amounts in Escherichia coli and was able to oxidize indole and indole derivatives into a variety of indigo compounds. The monooxygenase also performs the sulfoxidation of a wide range of prochiral sulfides, showing moderate to good enantioselectivities in forming chiral sulfoxides.

KW - Biocatalysis

KW - Escherichia coli

KW - Molecular Structure

KW - Oxidation-Reduction

KW - Oxygenases

KW - Substrate Specificity

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1039/c0ob00988a

DO - 10.1039/c0ob00988a

M3 - Journal article

C2 - 21225061

VL - 9

SP - 1337

EP - 1341

JO - Organic & Biomolecular Chemistry

JF - Organic & Biomolecular Chemistry

SN - 1477-0520

IS - 5

ER -

Rioz-Martínez A, Kopacz M, de Gonzalo G, Torres Pazmiño DE, Gotor V, Fraaije MW. Exploring the biocatalytic scope of a bacterial flavin-containing monooxygenase. Organic & Biomolecular Chemistry. 2011 mar 7;9(5):1337-41. https://doi.org/10.1039/c0ob00988a