Exogenous ether lipids predominantly target mitochondria

Lars Kuerschner, Doris Richter, Hans Kristian Hannibal-Bach, Anne Gaebler, Andrej Shevchenko, Christer S. Ejsing, Christoph Thiele

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

Ether lipids are ubiquitous constituents of cellular membranes with no discrete cell biological function assigned yet. Using fluorescent polyene-ether lipids we analyzed their intracellular distribution in living cells by microscopy. Mitochondria and the endoplasmic reticulum accumulated high amounts of ether-phosphatidylcholine and ether-phosphatidylethanolamine. Both lipids were specifically labeled using the corresponding lyso-ether lipids, which we established as supreme precursors for lipid tagging. Polyfosine, a fluorescent analogue of the anti-neoplastic ether lipid edelfosine, accumulated to mitochondria and induced morphological changes and cellular apoptosis. These data indicate that edelfosine could exert its pro-apoptotic power by targeting and damaging mitochondria and thereby inducing cellular apoptosis. In general, this study implies an important role of mitochondria in ether lipid metabolism and intracellular ether lipid trafficking.
OriginalsprogEngelsk
TidsskriftP L o S One
Vol/bind7
Udgave nummer2
Sider (fra-til)e31342
ISSN1932-6203
DOI
StatusUdgivet - 2012

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