Evidence that the medium-chain acyltransferase of lactating-goat mammary-gland fatty acid synthetase is identical with the acetyl/malonyltransferase

J Mikkelsen, P Højrup, H F Hansen, J K Hansen, J Knudsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Competitive binding experiments with malonyl-CoA and [1-14C]acetyl-CoA, [1-14C]butyryl-CoA or [1-14C]decanoyl-CoA indicate that all these substrates are transferred to lactating-goat mammary-gland fatty acid synthetase by the same transferase. Isolation and determination of the amino acid sequence of [1-14C]decanoyl-labelled CNBr-cleavage peptide from the decanoyltransferase site showed that this transferase is identical with the acetyl/malonyltransferase.

OriginalsprogEngelsk
TidsskriftThe Biochemical journal
Vol/bind227
Udgave nummer3
Sider (fra-til)981-5
Antal sider5
ISSN0264-6021
DOI
StatusUdgivet - 1. maj 1985

Fingeraftryk

Acyltransferases
Fatty Acid Synthases
Transferases
Malonyl Coenzyme A
Acetyl Coenzyme A
Protein Sequence Analysis
Amino Acids
Peptides
Substrates
Experiments
decanoyl-coenzyme A
butyryl-coenzyme A

Citer dette

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title = "Evidence that the medium-chain acyltransferase of lactating-goat mammary-gland fatty acid synthetase is identical with the acetyl/malonyltransferase",
abstract = "Competitive binding experiments with malonyl-CoA and [1-14C]acetyl-CoA, [1-14C]butyryl-CoA or [1-14C]decanoyl-CoA indicate that all these substrates are transferred to lactating-goat mammary-gland fatty acid synthetase by the same transferase. Isolation and determination of the amino acid sequence of [1-14C]decanoyl-labelled CNBr-cleavage peptide from the decanoyltransferase site showed that this transferase is identical with the acetyl/malonyltransferase.",
keywords = "Acetyl Coenzyme A/metabolism, Acyl Coenzyme A/metabolism, Acyltransferases/metabolism, Amino Acid Sequence, Animals, Binding Sites, Fatty Acid Synthases/metabolism, Female, Formates/metabolism, Goats, Lactation, Malonyl Coenzyme A/metabolism, Mammary Glands, Animal/enzymology, Pregnancy",
author = "J Mikkelsen and P H{\o}jrup and Hansen, {H F} and Hansen, {J K} and J Knudsen",
year = "1985",
month = "5",
day = "1",
doi = "10.1042/bj2270981",
language = "English",
volume = "227",
pages = "981--5",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
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Evidence that the medium-chain acyltransferase of lactating-goat mammary-gland fatty acid synthetase is identical with the acetyl/malonyltransferase. / Mikkelsen, J; Højrup, P; Hansen, H F; Hansen, J K; Knudsen, J.

I: The Biochemical journal, Bind 227, Nr. 3, 01.05.1985, s. 981-5.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Evidence that the medium-chain acyltransferase of lactating-goat mammary-gland fatty acid synthetase is identical with the acetyl/malonyltransferase

AU - Mikkelsen, J

AU - Højrup, P

AU - Hansen, H F

AU - Hansen, J K

AU - Knudsen, J

PY - 1985/5/1

Y1 - 1985/5/1

N2 - Competitive binding experiments with malonyl-CoA and [1-14C]acetyl-CoA, [1-14C]butyryl-CoA or [1-14C]decanoyl-CoA indicate that all these substrates are transferred to lactating-goat mammary-gland fatty acid synthetase by the same transferase. Isolation and determination of the amino acid sequence of [1-14C]decanoyl-labelled CNBr-cleavage peptide from the decanoyltransferase site showed that this transferase is identical with the acetyl/malonyltransferase.

AB - Competitive binding experiments with malonyl-CoA and [1-14C]acetyl-CoA, [1-14C]butyryl-CoA or [1-14C]decanoyl-CoA indicate that all these substrates are transferred to lactating-goat mammary-gland fatty acid synthetase by the same transferase. Isolation and determination of the amino acid sequence of [1-14C]decanoyl-labelled CNBr-cleavage peptide from the decanoyltransferase site showed that this transferase is identical with the acetyl/malonyltransferase.

KW - Acetyl Coenzyme A/metabolism

KW - Acyl Coenzyme A/metabolism

KW - Acyltransferases/metabolism

KW - Amino Acid Sequence

KW - Animals

KW - Binding Sites

KW - Fatty Acid Synthases/metabolism

KW - Female

KW - Formates/metabolism

KW - Goats

KW - Lactation

KW - Malonyl Coenzyme A/metabolism

KW - Mammary Glands, Animal/enzymology

KW - Pregnancy

U2 - 10.1042/bj2270981

DO - 10.1042/bj2270981

M3 - Journal article

C2 - 4004809

VL - 227

SP - 981

EP - 985

JO - Biochemical Journal

JF - Biochemical Journal

SN - 0264-6021

IS - 3

ER -