Evidence that the medium-chain acyltransferase of lactating-goat mammary-gland fatty acid synthetase is identical with the acetyl/malonyltransferase

J Mikkelsen, P Højrup, H F Hansen, J K Hansen, J Knudsen

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Abstract

Competitive binding experiments with malonyl-CoA and [1-14C]acetyl-CoA, [1-14C]butyryl-CoA or [1-14C]decanoyl-CoA indicate that all these substrates are transferred to lactating-goat mammary-gland fatty acid synthetase by the same transferase. Isolation and determination of the amino acid sequence of [1-14C]decanoyl-labelled CNBr-cleavage peptide from the decanoyltransferase site showed that this transferase is identical with the acetyl/malonyltransferase.

OriginalsprogEngelsk
TidsskriftBiochemical Journal
Vol/bind227
Udgave nummer3
Sider (fra-til)981-5
Antal sider5
ISSN0264-6021
DOI
StatusUdgivet - 1. maj 1985

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