Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant

S Douthwaite, J B Prince, H F Noller

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 1985-Dec
OriginalsprogEngelsk
TidsskriftProceedings of the National Academy of Science of the United States of America
Vol/bind82
Udgave nummer24
Sider (fra-til)8330-8334
Antal sider4
ISSN0027-8424
StatusUdgivet - 1985

Fingeraftryk

23S Ribosomal RNA
Mutation
Hydroxylamine
Ribosomal RNA
Microbial Drug Resistance
DNA Sequence Analysis
rRNA Genes

Citer dette

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title = "Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant",
abstract = "A mutation affording low levels of erythromycin resistance has been obtained by in vitro hydroxylamine mutagenesis of a cloned ribosomal RNA operon from Escherichia coli. The site of the mutational event responsible for antibiotic resistance was localized to the gene region encoding domain II of 23S rRNA by replacement of restriction fragments in the wild-type plasmid by corresponding fragments from the mutant plasmid. DNA sequencing showed that positions 1219-1230 of the 23S rRNA gene are deleted in the mutant. Since all previously characterized rRNA mutations conferring resistance to erythromycin show changes exclusively in domain V, our present findings provide direct evidence for functional interaction between domains II and V of 23S rRNA.",
keywords = "Ampicillin, Base Sequence, Chloramphenicol, Chromosome Mapping, Erythromycin, Escherichia coli, Nucleic Acid Conformation, Penicillin Resistance, Plasmids, RNA, Ribosomal",
author = "S Douthwaite and Prince, {J B} and Noller, {H F}",
year = "1985",
language = "English",
volume = "82",
pages = "8330--8334",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "24",

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Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant. / Douthwaite, S; Prince, J B; Noller, H F.

I: Proceedings of the National Academy of Science of the United States of America, Bind 82, Nr. 24, 1985, s. 8330-8334.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Evidence for functional interaction between domains II and V of 23S ribosomal RNA from an erythromycin-resistant mutant

AU - Douthwaite, S

AU - Prince, J B

AU - Noller, H F

PY - 1985

Y1 - 1985

N2 - A mutation affording low levels of erythromycin resistance has been obtained by in vitro hydroxylamine mutagenesis of a cloned ribosomal RNA operon from Escherichia coli. The site of the mutational event responsible for antibiotic resistance was localized to the gene region encoding domain II of 23S rRNA by replacement of restriction fragments in the wild-type plasmid by corresponding fragments from the mutant plasmid. DNA sequencing showed that positions 1219-1230 of the 23S rRNA gene are deleted in the mutant. Since all previously characterized rRNA mutations conferring resistance to erythromycin show changes exclusively in domain V, our present findings provide direct evidence for functional interaction between domains II and V of 23S rRNA.

AB - A mutation affording low levels of erythromycin resistance has been obtained by in vitro hydroxylamine mutagenesis of a cloned ribosomal RNA operon from Escherichia coli. The site of the mutational event responsible for antibiotic resistance was localized to the gene region encoding domain II of 23S rRNA by replacement of restriction fragments in the wild-type plasmid by corresponding fragments from the mutant plasmid. DNA sequencing showed that positions 1219-1230 of the 23S rRNA gene are deleted in the mutant. Since all previously characterized rRNA mutations conferring resistance to erythromycin show changes exclusively in domain V, our present findings provide direct evidence for functional interaction between domains II and V of 23S rRNA.

KW - Ampicillin

KW - Base Sequence

KW - Chloramphenicol

KW - Chromosome Mapping

KW - Erythromycin

KW - Escherichia coli

KW - Nucleic Acid Conformation

KW - Penicillin Resistance

KW - Plasmids

KW - RNA, Ribosomal

M3 - Journal article

VL - 82

SP - 8330

EP - 8334

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 24

ER -