Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release

Bidragets oversatte titel: Evaluering of kronisk spontan urticaria ved serum-induceret tumor necrosis factor alpha pg matril metalloproteinase 9 frigivelse

Sidsel Falkencrone, Carsten Bindslev-Jensen, Per Stahl Skov, Lars Kærgaard Poulsen, Marcus Maurer

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

Resumé

BACKGROUND
Previous studies from our group have demonstrated that IgE-mediated basophil activation leads to release of TNFα that in turn can induce matrix metallo-proteinase-9 (MMP-9) release from monocytes.
We wished to investigate if serum from chronic spontaneous urticaria-patients with auto-antibodies against IgE/IgE-receptor could induce TNFα and MMP-9 release from donor PBMCs, and if release levels could be used to assess severity and activity of chronic spontaneous urticaria (CSU).
METHODS
Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood from healthy donors and basophils isolated with MACS Basophil Isolation Kit to 97-99% purity. Cells were pulsed 1 hour with/without anti-IgE or with sera from CSU-patients/healthy controls and incubated for a total of 21 h before protein analysis of supernatants. MMP-9 and TNFα in supernatants were measured with commercial ELISAs (R&D), and histamine release determined with HR-test from RefLab ApS.
RESULTS
Stimulations with serum-pools demonstrated that CSU-serum, in contrast to healthy controls, was able to induce TNFα-release from isolated basophils.
10 sera from healthy controls and 22 sera from CSU-patients were tested for serum-induced histamine, TNFα and MMP-9 release. The CSU sera were grouped by reaction/no reaction in the autologous skin serum test (ASST), each group consisting of 11 sera.
9 of the 22 CSU-sera were found positive in the HR-test, 6 sera from ASST+ and 3 from ASST- patients. Sera from ASST+ and ASST- patients were observed to induce highly significant MMP-9 and TNFα release from donor PBMCs when compared to sera from healthy controls (p<0.001).
Urticaria assessment score (UAS) did not appear to correlate with release levels for histamine, TNFa or MMP-9 in either group but in the ASST+ group, the ASST score appeared to be positive correlated to histamine and TNFα release and to a smaller degree to MMP-9 release.
CONCLUSION
We have shown for the first time that serum from CSU-patients, in contrast to serum from healthy controls, can induce TNFα release from isolated basophils as well as TNFα and MMP-9 from donor PBMCS. Release levels appeared to be positive correlated to ASST reaction in ASST+ patients but not to disease severity for CSU patients in general.
OriginalsprogEngelsk
Publikationsdato12. jun. 2012
StatusUdgivet - 12. jun. 2012
BegivenhedXXVIII Congress of the European Academy of Allergy and Clinical Immunology - Warzaw, Polen
Varighed: 8. jun. 200910. jun. 2009

Konference

KonferenceXXVIII Congress of the European Academy of Allergy and Clinical Immunology
LandPolen
ByWarzaw
Periode08/06/200910/06/2009

Citer dette

Falkencrone, S., Bindslev-Jensen, C., Skov, P. S., Poulsen, L. K., & Maurer, M. (2012). Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release. Poster session præsenteret på XXVIII Congress of the European Academy of Allergy and Clinical Immunology, Warzaw, Polen.
Falkencrone, Sidsel ; Bindslev-Jensen, Carsten ; Skov, Per Stahl ; Poulsen, Lars Kærgaard ; Maurer, Marcus. / Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release. Poster session præsenteret på XXVIII Congress of the European Academy of Allergy and Clinical Immunology, Warzaw, Polen.
@conference{3b0c93599ce246419ec441435b741718,
title = "Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release",
abstract = "BACKGROUND Previous studies from our group have demonstrated that IgE-mediated basophil activation leads to release of TNFα that in turn can induce matrix metallo-proteinase-9 (MMP-9) release from monocytes. We wished to investigate if serum from chronic spontaneous urticaria-patients with auto-antibodies against IgE/IgE-receptor could induce TNFα and MMP-9 release from donor PBMCs, and if release levels could be used to assess severity and activity of chronic spontaneous urticaria (CSU). METHODS Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood from healthy donors and basophils isolated with MACS Basophil Isolation Kit to 97-99{\%} purity. Cells were pulsed 1 hour with/without anti-IgE or with sera from CSU-patients/healthy controls and incubated for a total of 21 h before protein analysis of supernatants. MMP-9 and TNFα in supernatants were measured with commercial ELISAs (R&D), and histamine release determined with HR-test from RefLab ApS. RESULTS Stimulations with serum-pools demonstrated that CSU-serum, in contrast to healthy controls, was able to induce TNFα-release from isolated basophils. 10 sera from healthy controls and 22 sera from CSU-patients were tested for serum-induced histamine, TNFα and MMP-9 release. The CSU sera were grouped by reaction/no reaction in the autologous skin serum test (ASST), each group consisting of 11 sera. 9 of the 22 CSU-sera were found positive in the HR-test, 6 sera from ASST+ and 3 from ASST- patients. Sera from ASST+ and ASST- patients were observed to induce highly significant MMP-9 and TNFα release from donor PBMCs when compared to sera from healthy controls (p<0.001). Urticaria assessment score (UAS) did not appear to correlate with release levels for histamine, TNFa or MMP-9 in either group but in the ASST+ group, the ASST score appeared to be positive correlated to histamine and TNFα release and to a smaller degree to MMP-9 release. CONCLUSION We have shown for the first time that serum from CSU-patients, in contrast to serum from healthy controls, can induce TNFα release from isolated basophils as well as TNFα and MMP-9 from donor PBMCS. Release levels appeared to be positive correlated to ASST reaction in ASST+ patients but not to disease severity for CSU patients in general.",
author = "Sidsel Falkencrone and Carsten Bindslev-Jensen and Skov, {Per Stahl} and Poulsen, {Lars K{\ae}rgaard} and Marcus Maurer",
year = "2012",
month = "6",
day = "12",
language = "English",
note = "null ; Conference date: 08-06-2009 Through 10-06-2009",

}

Falkencrone, S, Bindslev-Jensen, C, Skov, PS, Poulsen, LK & Maurer, M 2012, 'Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release' XXVIII Congress of the European Academy of Allergy and Clinical Immunology, Warzaw, Polen, 08/06/2009 - 10/06/2009, .

Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release. / Falkencrone, Sidsel; Bindslev-Jensen, Carsten; Skov, Per Stahl; Poulsen, Lars Kærgaard; Maurer, Marcus.

2012. Poster session præsenteret på XXVIII Congress of the European Academy of Allergy and Clinical Immunology, Warzaw, Polen.

Publikation: Konferencebidrag uden forlag/tidsskriftPosterForskning

TY - CONF

T1 - Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release

AU - Falkencrone, Sidsel

AU - Bindslev-Jensen, Carsten

AU - Skov, Per Stahl

AU - Poulsen, Lars Kærgaard

AU - Maurer, Marcus

PY - 2012/6/12

Y1 - 2012/6/12

N2 - BACKGROUND Previous studies from our group have demonstrated that IgE-mediated basophil activation leads to release of TNFα that in turn can induce matrix metallo-proteinase-9 (MMP-9) release from monocytes. We wished to investigate if serum from chronic spontaneous urticaria-patients with auto-antibodies against IgE/IgE-receptor could induce TNFα and MMP-9 release from donor PBMCs, and if release levels could be used to assess severity and activity of chronic spontaneous urticaria (CSU). METHODS Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood from healthy donors and basophils isolated with MACS Basophil Isolation Kit to 97-99% purity. Cells were pulsed 1 hour with/without anti-IgE or with sera from CSU-patients/healthy controls and incubated for a total of 21 h before protein analysis of supernatants. MMP-9 and TNFα in supernatants were measured with commercial ELISAs (R&D), and histamine release determined with HR-test from RefLab ApS. RESULTS Stimulations with serum-pools demonstrated that CSU-serum, in contrast to healthy controls, was able to induce TNFα-release from isolated basophils. 10 sera from healthy controls and 22 sera from CSU-patients were tested for serum-induced histamine, TNFα and MMP-9 release. The CSU sera were grouped by reaction/no reaction in the autologous skin serum test (ASST), each group consisting of 11 sera. 9 of the 22 CSU-sera were found positive in the HR-test, 6 sera from ASST+ and 3 from ASST- patients. Sera from ASST+ and ASST- patients were observed to induce highly significant MMP-9 and TNFα release from donor PBMCs when compared to sera from healthy controls (p<0.001). Urticaria assessment score (UAS) did not appear to correlate with release levels for histamine, TNFa or MMP-9 in either group but in the ASST+ group, the ASST score appeared to be positive correlated to histamine and TNFα release and to a smaller degree to MMP-9 release. CONCLUSION We have shown for the first time that serum from CSU-patients, in contrast to serum from healthy controls, can induce TNFα release from isolated basophils as well as TNFα and MMP-9 from donor PBMCS. Release levels appeared to be positive correlated to ASST reaction in ASST+ patients but not to disease severity for CSU patients in general.

AB - BACKGROUND Previous studies from our group have demonstrated that IgE-mediated basophil activation leads to release of TNFα that in turn can induce matrix metallo-proteinase-9 (MMP-9) release from monocytes. We wished to investigate if serum from chronic spontaneous urticaria-patients with auto-antibodies against IgE/IgE-receptor could induce TNFα and MMP-9 release from donor PBMCs, and if release levels could be used to assess severity and activity of chronic spontaneous urticaria (CSU). METHODS Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood from healthy donors and basophils isolated with MACS Basophil Isolation Kit to 97-99% purity. Cells were pulsed 1 hour with/without anti-IgE or with sera from CSU-patients/healthy controls and incubated for a total of 21 h before protein analysis of supernatants. MMP-9 and TNFα in supernatants were measured with commercial ELISAs (R&D), and histamine release determined with HR-test from RefLab ApS. RESULTS Stimulations with serum-pools demonstrated that CSU-serum, in contrast to healthy controls, was able to induce TNFα-release from isolated basophils. 10 sera from healthy controls and 22 sera from CSU-patients were tested for serum-induced histamine, TNFα and MMP-9 release. The CSU sera were grouped by reaction/no reaction in the autologous skin serum test (ASST), each group consisting of 11 sera. 9 of the 22 CSU-sera were found positive in the HR-test, 6 sera from ASST+ and 3 from ASST- patients. Sera from ASST+ and ASST- patients were observed to induce highly significant MMP-9 and TNFα release from donor PBMCs when compared to sera from healthy controls (p<0.001). Urticaria assessment score (UAS) did not appear to correlate with release levels for histamine, TNFa or MMP-9 in either group but in the ASST+ group, the ASST score appeared to be positive correlated to histamine and TNFα release and to a smaller degree to MMP-9 release. CONCLUSION We have shown for the first time that serum from CSU-patients, in contrast to serum from healthy controls, can induce TNFα release from isolated basophils as well as TNFα and MMP-9 from donor PBMCS. Release levels appeared to be positive correlated to ASST reaction in ASST+ patients but not to disease severity for CSU patients in general.

M3 - Poster

ER -

Falkencrone S, Bindslev-Jensen C, Skov PS, Poulsen LK, Maurer M. Assessment of chronic spontaneous urticaria by serum-induced tumor necrosis factor alpha and matrix metalloproteinase-9 release. 2012. Poster session præsenteret på XXVIII Congress of the European Academy of Allergy and Clinical Immunology, Warzaw, Polen.