Evaluation of siRNA stability and interaction with serum components using an agarose gel-based single-molecule FRET labeling method

Martina Tuttolomondo*, Henrik J. Ditzel

*Kontaktforfatter for dette arbejde

Publikation: Kapitel i bog/rapport/konference-proceedingKapitel i bogForskningpeer review

Abstrakt

Small interfering RNAs (siRNAs) are RNA molecules with promising therapeutic potential as a result of their selective mRNA cleavage. However, despite recent progress, low stability in the bloodstream is an impediment to successful administration in vivo. Thus, the availability of flexible and rapid methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast Förster resonance energy transfer (FRET) method based on agarose gel electrophoresis to evaluate the stability of siRNA in serum as well as siRNA interaction with serum proteins and enzymes.

OriginalsprogEngelsk
TitelDesign and Delivery of SiRNA Therapeutics
RedaktørerHenrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen
Vol/bind2282
ForlagHumana Press
Publikationsdato2021
Sider43-56
ISBN (Trykt) 978-1-0716-1297-2
ISBN (Elektronisk)978-1-0716-1298-9
DOI
StatusUdgivet - 2021
NavnMethods in Molecular Biology
Vol/bind2282
ISSN1064-3745

Bibliografisk note

Funding Information:
We gratefully acknowledge funding support from A Race Agaisnt Breast Cancer and the A.P. Moeller Foundation. Moreover, we acknowledge Dr. Bartosz Pilecki from the Department of Cancer and Inflammation Research, University of Southern Denmark, for providing the mouse serum.

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2021.

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