Small interfering RNAs (siRNAs) are RNA molecules with promising therapeutic potential as a result of their selective mRNA cleavage. However, despite recent progress, low stability in the bloodstream is an impediment to successful administration in vivo. Thus, the availability of flexible and rapid methods for studying siRNA stability and vehicles is crucial for future novel siRNA-based therapeutics. Herein, we report a fast Förster resonance energy transfer (FRET) method based on agarose gel electrophoresis to evaluate the stability of siRNA in serum as well as siRNA interaction with serum proteins and enzymes.
|Titel||Design and Delivery of SiRNA Therapeutics|
|Redaktører||Henrik J. Ditzel, Martina Tuttolomondo, Sakari Kauppinen|
|Status||Udgivet - 2021|
|Navn||Methods in Molecular Biology|
Bibliografisk noteFunding Information:
We gratefully acknowledge funding support from A Race Agaisnt Breast Cancer and the A.P. Moeller Foundation. Moreover, we acknowledge Dr. Bartosz Pilecki from the Department of Cancer and Inflammation Research, University of Southern Denmark, for providing the mouse serum.
© Springer Science+Business Media, LLC, part of Springer Nature 2021.