ESwab challenges influenza virus propagation in cell cultures

Ramona Trebbien, B Andersen, Jesper Rønn, Jacob L McCauley, T Kølsen Fischer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

Although the ESwab kit (Copan, Brescia, Italy) is intended for sampling bacteria for culture, this kit is increasingly also used for virus sampling. The effect of ESwab medium on influenza virus detection by real-time reverse transcription-polymerase chain reaction (RT-PCR) or virus propagation in Madin-Darby canine kidney (MDCK) cell culture was investigated. The ESwab medium was suitable for viral RNA detection but not for viral propagation due to cytotoxicity. Sampling influenza viruses with ESwab challenges influenza surveillance by strongly limiting the possibility of antigenic characterisation.

OriginalsprogEngelsk
TidsskriftEurosurveillance (Online Edition)
Vol/bind19
Udgave nummer50
Sider (fra-til)20995
ISSN1025-496X
DOI
StatusUdgivet - 18. dec. 2014
Udgivet eksterntJa

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