ESwab challenges influenza virus propagation in cell cultures

Ramona Trebbien, B Andersen, Jesper Rønn, Jacob L McCauley, T Kølsen Fischer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Although the ESwab kit (Copan, Brescia, Italy) is intended for sampling bacteria for culture, this kit is increasingly also used for virus sampling. The effect of ESwab medium on influenza virus detection by real-time reverse transcription-polymerase chain reaction (RT-PCR) or virus propagation in Madin-Darby canine kidney (MDCK) cell culture was investigated. The ESwab medium was suitable for viral RNA detection but not for viral propagation due to cytotoxicity. Sampling influenza viruses with ESwab challenges influenza surveillance by strongly limiting the possibility of antigenic characterisation.

OriginalsprogEngelsk
TidsskriftEurosurveillance (Online Edition)
Vol/bind19
Udgave nummer50
Sider (fra-til)20995
ISSN1025-496X
DOI
StatusUdgivet - 18. dec. 2014
Udgivet eksterntJa

Fingeraftryk

Orthomyxoviridae
Viruses
Madin Darby Canine Kidney Cells
Viral RNA
Human Influenza
Italy
Polymerase Chain Reaction

Citer dette

Trebbien, R., Andersen, B., Rønn, J., McCauley, J. L., & Fischer, T. K. (2014). ESwab challenges influenza virus propagation in cell cultures. Eurosurveillance (Online Edition), 19(50), 20995. https://doi.org/10.2807/1560-7917.ES2014.19.50.20995
Trebbien, Ramona ; Andersen, B ; Rønn, Jesper ; McCauley, Jacob L ; Fischer, T Kølsen. / ESwab challenges influenza virus propagation in cell cultures. I: Eurosurveillance (Online Edition). 2014 ; Bind 19, Nr. 50. s. 20995.
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title = "ESwab challenges influenza virus propagation in cell cultures",
abstract = "Although the ESwab kit (Copan, Brescia, Italy) is intended for sampling bacteria for culture, this kit is increasingly also used for virus sampling. The effect of ESwab medium on influenza virus detection by real-time reverse transcription-polymerase chain reaction (RT-PCR) or virus propagation in Madin-Darby canine kidney (MDCK) cell culture was investigated. The ESwab medium was suitable for viral RNA detection but not for viral propagation due to cytotoxicity. Sampling influenza viruses with ESwab challenges influenza surveillance by strongly limiting the possibility of antigenic characterisation.",
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Trebbien, R, Andersen, B, Rønn, J, McCauley, JL & Fischer, TK 2014, 'ESwab challenges influenza virus propagation in cell cultures', Eurosurveillance (Online Edition), bind 19, nr. 50, s. 20995. https://doi.org/10.2807/1560-7917.ES2014.19.50.20995

ESwab challenges influenza virus propagation in cell cultures. / Trebbien, Ramona; Andersen, B; Rønn, Jesper; McCauley, Jacob L; Fischer, T Kølsen.

I: Eurosurveillance (Online Edition), Bind 19, Nr. 50, 18.12.2014, s. 20995.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - ESwab challenges influenza virus propagation in cell cultures

AU - Trebbien, Ramona

AU - Andersen, B

AU - Rønn, Jesper

AU - McCauley, Jacob L

AU - Fischer, T Kølsen

PY - 2014/12/18

Y1 - 2014/12/18

N2 - Although the ESwab kit (Copan, Brescia, Italy) is intended for sampling bacteria for culture, this kit is increasingly also used for virus sampling. The effect of ESwab medium on influenza virus detection by real-time reverse transcription-polymerase chain reaction (RT-PCR) or virus propagation in Madin-Darby canine kidney (MDCK) cell culture was investigated. The ESwab medium was suitable for viral RNA detection but not for viral propagation due to cytotoxicity. Sampling influenza viruses with ESwab challenges influenza surveillance by strongly limiting the possibility of antigenic characterisation.

AB - Although the ESwab kit (Copan, Brescia, Italy) is intended for sampling bacteria for culture, this kit is increasingly also used for virus sampling. The effect of ESwab medium on influenza virus detection by real-time reverse transcription-polymerase chain reaction (RT-PCR) or virus propagation in Madin-Darby canine kidney (MDCK) cell culture was investigated. The ESwab medium was suitable for viral RNA detection but not for viral propagation due to cytotoxicity. Sampling influenza viruses with ESwab challenges influenza surveillance by strongly limiting the possibility of antigenic characterisation.

KW - Animals

KW - Cell Culture Techniques

KW - DNA, Viral

KW - Dogs

KW - Madin Darby Canine Kidney Cells

KW - Orthomyxoviridae

KW - Orthomyxoviridae Infections

KW - RNA, Viral

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.2807/1560-7917.ES2014.19.50.20995

DO - 10.2807/1560-7917.ES2014.19.50.20995

M3 - Journal article

C2 - 25597539

VL - 19

SP - 20995

JO - Eurosurveillance

JF - Eurosurveillance

SN - 1025-496X

IS - 50

ER -