Estrone-1-sulphate (E1S) has impact on the kinetics parameters of transporter mediated taurine and glutamate influx in Caco-2 cells

Bente Steffansen, F El-Sayed

Publikation: Konferencebidrag uden forlag/tidsskriftKonferenceabstrakt til konferenceForskningpeer review

Abstrakt

Previously, we have suggested estrone-1-sulfate (E1S) to be intercalated into the phospholipid membrane 1,2-dipalmitoyl-sn-glycero-3-phospho-choline (DPPC).
The overall hypothesis of the present study was that E1S intercalation in the cell membrane of Caco-2 cells may changes the functionality of membrane transporters.
The aim was therefore to investigate if addition of E1S to the growth medium of Caco-2 cells before but not during the influx study, change the kinetic parameters of transporter-mediated influx of taurine and glutamate by respective TAUT and EAAT transporters.
The results show that 4 days pretreatment with E1S change the concentration dependent influx curves and Km for transporter mediated taurine and Km and Jmax for glutamate influx although the effects on Km and Jmax are not significant.
OriginalsprogEngelsk
Publikationsdato2015
StatusUdgivet - 2015
BegivenhedThe 42nd Annual Meeting and Exposition of the Controlled Release Society - Edinburgh International Conference Centre, Edinburgh, Storbritannien
Varighed: 26. jul. 201529. jul. 2015

Konference

KonferenceThe 42nd Annual Meeting and Exposition of the Controlled Release Society
LokationEdinburgh International Conference Centre
LandStorbritannien
ByEdinburgh
Periode26/07/201529/07/2015

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