Estrone-1-sulphate (E1S) has impact on the kinetics parameters of transporter mediated taurine and glutamate influx in Caco-2 cells

Bente Steffansen; F El-Sayed

Estrone-1-sulphate (E1S) has impact on the kinetics parameters of transporter mediated taurine and glutamate influx in Caco-2 cells

Bente Steffansen, F El-Sayed

Institut for Fysik, Kemi og Farmaci

Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Forskning › peer review

Abstrakt

Previously, we have suggested estrone-1-sulfate (E1S) to be intercalated into the phospholipid membrane 1,2-dipalmitoyl-sn-glycero-3-phospho-choline (DPPC).
The overall hypothesis of the present study was that E1S intercalation in the cell membrane of Caco-2 cells may changes the functionality of membrane transporters.
The aim was therefore to investigate if addition of E1S to the growth medium of Caco-2 cells before but not during the influx study, change the kinetic parameters of transporter-mediated influx of taurine and glutamate by respective TAUT and EAAT transporters.
The results show that 4 days pretreatment with E1S change the concentration dependent influx curves and Km for transporter mediated taurine and Km and Jmax for glutamate influx although the effects on Km and Jmax are not significant.

Originalsprog	Engelsk
Publikationsdato	2015
Status	Udgivet - 2015
Begivenhed	The 42nd Annual Meeting and Exposition of the Controlled Release Society - Edinburgh International Conference Centre, Edinburgh, Storbritannien Varighed: 26. jul. 2015 → 29. jul. 2015

Konference

Konference	The 42nd Annual Meeting and Exposition of the Controlled Release Society
Lokation	Edinburgh International Conference Centre
Land	Storbritannien
By	Edinburgh
Periode	26/07/2015 → 29/07/2015

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Citationsformater

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title = "Estrone-1-sulphate (E1S) has impact on the kinetics parameters of transporter mediated taurine and glutamate influx in Caco-2 cells",

abstract = "Previously, we have suggested estrone-1-sulfate (E1S) to be intercalated into the phospholipid membrane 1,2-dipalmitoyl-sn-glycero-3-phospho-choline (DPPC). The overall hypothesis of the present study was that E1S intercalation in the cell membrane of Caco-2 cells may changes the functionality of membrane transporters.The aim was therefore to investigate if addition of E1S to the growth medium of Caco-2 cells before but not during the influx study, change the kinetic parameters of transporter-mediated influx of taurine and glutamate by respective TAUT and EAAT transporters. The results show that 4 days pretreatment with E1S change the concentration dependent influx curves and Km for transporter mediated taurine and Km and Jmax for glutamate influx although the effects on Km and Jmax are not significant. ",

author = "Bente Steffansen and F El-Sayed",

year = "2015",

language = "English",

note = "null ; Conference date: 26-07-2015 Through 29-07-2015",

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Estrone-1-sulphate (E1S) has impact on the kinetics parameters of transporter mediated taurine and glutamate influx in Caco-2 cells. / Steffansen, Bente; El-Sayed, F.

2015. Abstract fra The 42nd Annual Meeting and Exposition of the Controlled Release Society, Edinburgh, Storbritannien.

Publikation: Konferencebidrag uden forlag/tidsskrift › Konferenceabstrakt til konference › Forskning › peer review

TY - ABST

T1 - Estrone-1-sulphate (E1S) has impact on the kinetics parameters of transporter mediated taurine and glutamate influx in Caco-2 cells

AU - Steffansen, Bente

AU - El-Sayed, F

PY - 2015

Y1 - 2015

N2 - Previously, we have suggested estrone-1-sulfate (E1S) to be intercalated into the phospholipid membrane 1,2-dipalmitoyl-sn-glycero-3-phospho-choline (DPPC). The overall hypothesis of the present study was that E1S intercalation in the cell membrane of Caco-2 cells may changes the functionality of membrane transporters.The aim was therefore to investigate if addition of E1S to the growth medium of Caco-2 cells before but not during the influx study, change the kinetic parameters of transporter-mediated influx of taurine and glutamate by respective TAUT and EAAT transporters. The results show that 4 days pretreatment with E1S change the concentration dependent influx curves and Km for transporter mediated taurine and Km and Jmax for glutamate influx although the effects on Km and Jmax are not significant.

AB - Previously, we have suggested estrone-1-sulfate (E1S) to be intercalated into the phospholipid membrane 1,2-dipalmitoyl-sn-glycero-3-phospho-choline (DPPC). The overall hypothesis of the present study was that E1S intercalation in the cell membrane of Caco-2 cells may changes the functionality of membrane transporters.The aim was therefore to investigate if addition of E1S to the growth medium of Caco-2 cells before but not during the influx study, change the kinetic parameters of transporter-mediated influx of taurine and glutamate by respective TAUT and EAAT transporters. The results show that 4 days pretreatment with E1S change the concentration dependent influx curves and Km for transporter mediated taurine and Km and Jmax for glutamate influx although the effects on Km and Jmax are not significant.

M3 - Conference abstract for conference

Y2 - 26 July 2015 through 29 July 2015

ER -