Enzymes Immobilized on Carbon Nitride (C₃N₄) Cooperating with Metal Nanoparticles for Cascade Catalysis

The exploration of effective platforms for immobilizing chemo- and biocatalysts to develop biohybrid catalysts is an attractive subject of practical interest. In this work, carbon nitride (C₃N₄) is used for the first time as a platform for the immobilization of metal catalyst (Pd nanoparticles) and biocatalyst (Candida antarctica lipase B, CalB) in a facile manner to prepare biohybrid catalyst. The optimal biohybrid catalyst inherits the intrinsic performance of both Pd nanoparticles and CalB, and shows high activity in the one-pot cascade reaction converting benzaldehyde to benzyl hexanoate at room temperature. With this proof of concept, it is expected that C₃N₄ can be utilized for immobilizing more types of chemo- and biocatalysts for perspective applications.