Enzyme-linked immunosorbent assay characterization of Basal variation and heritability of systemic microfibrillar-associated protein 4

Susanne Gjørup Sækmose, Anders Schlosser, René Holst, Sofie Lock Johansson, Helle Wulf-Johansson, Ida Tornøe, Jørgen Vestbo, Kirsten Ohm Kyvik, Torben Barington, Uffe Holmskov, Grith Lykke Sørensen

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Abstrakt

Microfibrillar-associated protein 4 (MFAP4) is a systemic biomarker that is significantly elevated in samples from patients suffering from hepatic cirrhosis. The protein is generally localized to elastic fibers and other connective tissue fibers in the extracellular matrix (ECM), and variation in systemic MFAP4 (sMFAP4) has the potential to reflect diverse diseases with increased ECM turnover. Here, we aimed to validate an enzyme-linked immunosorbent assay (ELISA) for the measurement of sMFAP4 with an emphasis on the robustness of the assay. Moreover, we aimed to determine confounders influencing the basal sMFAP4 variability and the genetic contribution to the basal variation.
OriginalsprogEngelsk
Artikelnummere82383
TidsskriftPLOS ONE
Vol/bind8
Udgave nummer12
Sider (fra-til)e82383
Antal sider13
ISSN1932-6203
DOI
StatusUdgivet - 4. dec. 2013

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