Enzyme immunoassay of oestrogen receptors in needle biopsies from human liver

Ulrik Becker*, Jørn Andersen, Hans Skovgaard Poulsen, Flemming Burcharth, Christian Gluud, Thomas Horn

*Kontaktforfatter for dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

ABSTRACT— For quantitative assessments of sex hormone receptors in liver tissue, ligand binding assays are inconvenient, as they require large biopsies (0.5–1.0 g). The present study shows that it is possible to measure oestrogen receptors (ER) quantitatively in needle biopsy specimens as small as 10 mg by modifications of a commercial enzyme immunoassay employing monoclonal antibodies. Sucrose gradient centrifugation and the dextran charcoal method served as reference methods. A consecutive series of needle biopsies from patients suspected of liver disease were investigated. The biopsies (n = 37) had a median weight of 14 mg and cytosolic protein concentrations > 1 mg/ml (median 1.28 mg/ml). The median ER concentration was 20 fmol/mg cytosolic protein (range 5 to 57 fmol/mg). The intra‐assay coefficient of variation was 8.9%, the inter‐assay 13.2%, and the detection limit 2.7 fmol/ml cytosol. Women had significantly higher ER concentrations (median 22 fmol/mg) compared to male patients (median 16 fmol/mg) (P = 0.007). The enzyme immunoassay measures ER in liver specimens as small as 10 mg, compared to the large tissue specimens necessary for the conventional DCC assay, and the method is a convenient tool for further studies of ER in routine needle biopsies from the liver.

OriginalsprogEngelsk
TidsskriftLiver
Vol/bind11
Udgave nummer5
Sider (fra-til)292-299
Antal sider8
ISSN0106-9543
DOI
StatusUdgivet - 1. jan. 1991

Fingeraftryk

Liver
Charcoal
Centrifugation
Cytosol
Limit of Detection
Liver Diseases
Proteins
Ligands
Weights and Measures

Citer dette

Becker, U., Andersen, J., Poulsen, H. S., Burcharth, F., Gluud, C., & Horn, T. (1991). Enzyme immunoassay of oestrogen receptors in needle biopsies from human liver. Liver, 11(5), 292-299. https://doi.org/10.1111/j.1600-0676.1991.tb00532.x
Becker, Ulrik ; Andersen, Jørn ; Poulsen, Hans Skovgaard ; Burcharth, Flemming ; Gluud, Christian ; Horn, Thomas. / Enzyme immunoassay of oestrogen receptors in needle biopsies from human liver. I: Liver. 1991 ; Bind 11, Nr. 5. s. 292-299.
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title = "Enzyme immunoassay of oestrogen receptors in needle biopsies from human liver",
abstract = "ABSTRACT— For quantitative assessments of sex hormone receptors in liver tissue, ligand binding assays are inconvenient, as they require large biopsies (0.5–1.0 g). The present study shows that it is possible to measure oestrogen receptors (ER) quantitatively in needle biopsy specimens as small as 10 mg by modifications of a commercial enzyme immunoassay employing monoclonal antibodies. Sucrose gradient centrifugation and the dextran charcoal method served as reference methods. A consecutive series of needle biopsies from patients suspected of liver disease were investigated. The biopsies (n = 37) had a median weight of 14 mg and cytosolic protein concentrations > 1 mg/ml (median 1.28 mg/ml). The median ER concentration was 20 fmol/mg cytosolic protein (range 5 to 57 fmol/mg). The intra‐assay coefficient of variation was 8.9{\%}, the inter‐assay 13.2{\%}, and the detection limit 2.7 fmol/ml cytosol. Women had significantly higher ER concentrations (median 22 fmol/mg) compared to male patients (median 16 fmol/mg) (P = 0.007). The enzyme immunoassay measures ER in liver specimens as small as 10 mg, compared to the large tissue specimens necessary for the conventional DCC assay, and the method is a convenient tool for further studies of ER in routine needle biopsies from the liver.",
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Becker, U, Andersen, J, Poulsen, HS, Burcharth, F, Gluud, C & Horn, T 1991, 'Enzyme immunoassay of oestrogen receptors in needle biopsies from human liver', Liver, bind 11, nr. 5, s. 292-299. https://doi.org/10.1111/j.1600-0676.1991.tb00532.x

Enzyme immunoassay of oestrogen receptors in needle biopsies from human liver. / Becker, Ulrik; Andersen, Jørn; Poulsen, Hans Skovgaard; Burcharth, Flemming; Gluud, Christian; Horn, Thomas.

I: Liver, Bind 11, Nr. 5, 01.01.1991, s. 292-299.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Enzyme immunoassay of oestrogen receptors in needle biopsies from human liver

AU - Becker, Ulrik

AU - Andersen, Jørn

AU - Poulsen, Hans Skovgaard

AU - Burcharth, Flemming

AU - Gluud, Christian

AU - Horn, Thomas

PY - 1991/1/1

Y1 - 1991/1/1

N2 - ABSTRACT— For quantitative assessments of sex hormone receptors in liver tissue, ligand binding assays are inconvenient, as they require large biopsies (0.5–1.0 g). The present study shows that it is possible to measure oestrogen receptors (ER) quantitatively in needle biopsy specimens as small as 10 mg by modifications of a commercial enzyme immunoassay employing monoclonal antibodies. Sucrose gradient centrifugation and the dextran charcoal method served as reference methods. A consecutive series of needle biopsies from patients suspected of liver disease were investigated. The biopsies (n = 37) had a median weight of 14 mg and cytosolic protein concentrations > 1 mg/ml (median 1.28 mg/ml). The median ER concentration was 20 fmol/mg cytosolic protein (range 5 to 57 fmol/mg). The intra‐assay coefficient of variation was 8.9%, the inter‐assay 13.2%, and the detection limit 2.7 fmol/ml cytosol. Women had significantly higher ER concentrations (median 22 fmol/mg) compared to male patients (median 16 fmol/mg) (P = 0.007). The enzyme immunoassay measures ER in liver specimens as small as 10 mg, compared to the large tissue specimens necessary for the conventional DCC assay, and the method is a convenient tool for further studies of ER in routine needle biopsies from the liver.

AB - ABSTRACT— For quantitative assessments of sex hormone receptors in liver tissue, ligand binding assays are inconvenient, as they require large biopsies (0.5–1.0 g). The present study shows that it is possible to measure oestrogen receptors (ER) quantitatively in needle biopsy specimens as small as 10 mg by modifications of a commercial enzyme immunoassay employing monoclonal antibodies. Sucrose gradient centrifugation and the dextran charcoal method served as reference methods. A consecutive series of needle biopsies from patients suspected of liver disease were investigated. The biopsies (n = 37) had a median weight of 14 mg and cytosolic protein concentrations > 1 mg/ml (median 1.28 mg/ml). The median ER concentration was 20 fmol/mg cytosolic protein (range 5 to 57 fmol/mg). The intra‐assay coefficient of variation was 8.9%, the inter‐assay 13.2%, and the detection limit 2.7 fmol/ml cytosol. Women had significantly higher ER concentrations (median 22 fmol/mg) compared to male patients (median 16 fmol/mg) (P = 0.007). The enzyme immunoassay measures ER in liver specimens as small as 10 mg, compared to the large tissue specimens necessary for the conventional DCC assay, and the method is a convenient tool for further studies of ER in routine needle biopsies from the liver.

KW - dextran‐coated charcoal assay

KW - enzyme immunoassay

KW - estrogen receptors

KW - liver

KW - sucrose gradient centrifugation

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DO - 10.1111/j.1600-0676.1991.tb00532.x

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JO - Liver International

JF - Liver International

SN - 1478-3223

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