The triphosphate of the thymine derivative of 2'-amino-LNA (2'-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion® HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template. To complement this, 2'-amino-LNA-T phosphoramidites were incorporated into DNA oligonucleotides which were used as templates for enzymatic synthesis of unmodified DNA using either KOD, KOD XL or Phusion polymerases. 2'-Amino-LNA-T in the template and 2'-amino-LNA-TTP as a substrate both decreased reaction rate and yield compared to unmodified DNA, especially for sequences with multiple 2'-amino-LNA-T nucleotides.
Johannsen, M. W., Veedu, R. N., Madsen, A. S., & Wengel, J. (2012). Enzymatic polymerisation involving 2'-amino-LNA nucleotides. Bioorganic & Medicinal Chemistry Letters, 22(10), 3522-3526. https://doi.org/10.1016/j.bmcl.2012.03.073