Enzymatic polymerisation involving 2'-amino-LNA nucleotides

Marie W Johannsen, Rakesh N Veedu, Andreas Stahl Madsen, Jesper Wengel

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Abstrakt

The triphosphate of the thymine derivative of 2'-amino-LNA (2'-amino-LNA-TTP) was synthesised and found to be a good substrate for Phusion® HF DNA polymerase, allowing enzymatic synthesis of modified DNA encoded by an unmodified template. To complement this, 2'-amino-LNA-T phosphoramidites were incorporated into DNA oligonucleotides which were used as templates for enzymatic synthesis of unmodified DNA using either KOD, KOD XL or Phusion polymerases. 2'-Amino-LNA-T in the template and 2'-amino-LNA-TTP as a substrate both decreased reaction rate and yield compared to unmodified DNA, especially for sequences with multiple 2'-amino-LNA-T nucleotides.
OriginalsprogEngelsk
TidsskriftBioorganic & Medicinal Chemistry Letters
Vol/bind22
Udgave nummer10
Sider (fra-til)3522-3526
Antal sider5
ISSN0960-894X
DOI
StatusUdgivet - 2012

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