Enhanced H-bonding and pi-stacking in DNA: a potent duplex-stabilizing and mismatch sensing nucleobase analogue

Chenguang Lou, Andre Dallmann, Pietro Marafini, Rachel Gao, Tom Brown

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Abstrakt

X-pyrene is a new nucleic acid duplex stabilizing cytosine analogue that combines enhanced pi-stacking, hydrogen bonding and electrostatic interactions to greatly increase the stability of bulged DNA duplexes and DNA/RNA hybrids. X-pyrene is highly selective for guanine as a partner and duplex stability is reduced dramatically when X-pyrene or a neighboring base is mismatched. An NMR study indicates that the pyrene moiety stacks within the helix, and large changes in fluorescence emission on duplex formation are consistent with this. These favorable properties make X-pyrene a promising cytosine analogue for use in a variety of biological applications.
OriginalsprogEngelsk
TidsskriftChemical Science
Vol/bind5
Udgave nummer10
Sider (fra-til)3836-3844
Antal sider9
ISSN2041-6520
DOI
StatusUdgivet - aug. 2014

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