Endothelin-1 shifts the mediator of bradykinin-induced relaxation from NO to H2 O2 in resistance arteries from patients with cardiovascular disease

Thomas M Leurgans, Maria Bloksgaard, Jonathan R Brewer, Luis A Bagatolli, Maise H Fredgart, Kristoffer Rosenstand, Maria L Hansen, Lars M Rasmussen, Akhmadjon Irmukhamedov, Jo G R De Mey

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Background and Purpose We tested the hypothesis that in resistance arteries from cardiovascular disease (CVD) patients, effects of an endothelium-dependent vasodilator depend on the contractile stimulus. Experimental Approach Arteries dissected from parietal pericardium of cardiothoracic surgery patients were studied by myography and imaging techniques. Segments were sub-maximally contracted by K +, the TxA 2 analogue U46619 or endothelin-1 (ET-1). Key Results Relaxing effects of Na-nitroprusside were comparable, but those of bradykinin (BK) were bigger in the presence of ET-1 compared with K + or U46619. BK-induced relaxation was (i) abolished by L-NAME in K +-contracted arteries, (ii) partly inhibited by L-NAME in the presence of U46619 and (iii) not altered by indomethacin, L-NAME plus inhibitors of small and intermediate conductance calcium-activated K + channels, but attenuated by catalase, in ET-1-contracted arteries. This catalase-sensitive relaxation was unaffected by inhibitors of NADPH oxidases or allopurinol. Exogenous H 2O 2 caused a larger relaxation of ET-1-induced contractions than those evoked by K + or U46619 in the presence of inhibitors of other endothelium-derived relaxing factors. Catalase-sensitive staining of cellular ROS with CellROX Deep Red was significantly increased in the presence of both 1 μM BK and 2 nM ET-1 but not either peptide alone. Conclusions and Implications In resistance arteries from patients with CVD, exogenous ET-1 shifts the mediator of relaxing responses to the endothelium-dependent vasodilator BK from NO to H 2O 2 and neither NADPH oxidases, xanthine oxidase nor NOS appear to be involved in this effect. This might have consequences for endothelial dysfunction in conditions where intra-arterial levels of ET-1 are enhanced.

TidsskriftBritish Journal of Pharmacology
Udgave nummer10
Sider (fra-til)1653-1664
StatusUdgivet - maj 2016


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