Endocrine-disrupting effect of the ultraviolet filter benzophenone-3 in zebrafish, Danio rerio

Karin Lund Kinnberg, Gitte I. Petersen, Mette Albrektsen, Mite Minghlani, Suad Mohamud Awad, Bente Frost Holbech, John W. Green, Poul Bjerregaard, Henrik Holbech

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The chemical ultraviolet (UV) filter benzophenone-3 (BP-3) is suspected to be an endocrine disruptor based on results from in vitro and in vivo testing. However, studies including endpoints of endocrine adversity are lacking. The present study investigated the potential endocrine-disrupting effects of BP-3 in zebrafish (Danio rerio) in the Fish Sexual Development Test (Organisation for Economic Co-operation and Development TG 234) and a 12 day adult male zebrafish study. In TG 234, exposure from 0 to 60 d posthatch caused a monotone dose-dependent skewing of the phenotypic sex ratio towards less males and more female zebrafish (no observed effect concentration [NOEC]: 191 µg/L, lowest observed effect concentration [LOEC]: 388 µg/L). Besides, gonad maturation was affected in both female fish (NOEC 191 µg/L, LOEC 388 µg/L) and male fish (NOEC 388 µg/L, LOEC 470 µg/L). Exposure to BP-3 did not affect the vitellogenin concentration in TG 234. After 12 d exposure of adult male zebrafish, a slight but yet significant increase in the vitellogenin concentration was observed at 268 µg/L but not at 63 µg/L and 437 µg/l BP-3. Skewing of the sex ratio is a marker of an endocrine mediated mechanism as well as a marker of adversity and therefore the conclusion of the investigation is that BP-3 is an endocrine-disrupting chemical in accordance with the World Health Organization's definition.
OriginalsprogEngelsk
TidsskriftEnvironmental Toxicology and Chemistry
Vol/bind34
Udgave nummer12
Sider (fra-til)2833-2840
Antal sider8
ISSN0730-7268
DOI
StatusUdgivet - 2015

Fingeraftryk

Zebrafish
dose-response relationship
filter
Fish
Vitellogenins
Endocrine Disruptors
sex ratio
Sex Ratio
fish
Fishes
endocrine disruptor
World Health Organization
OECD
Gonads
maturation
effect
oxybenzone
Health
Economics
exposure

Citer dette

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title = "Endocrine-disrupting effect of the ultraviolet filter benzophenone-3 in zebrafish, Danio rerio",
abstract = "The chemical ultraviolet (UV) filter benzophenone-3 (BP-3) is suspected to be an endocrine disruptor based on results from in vitro and in vivo testing. However, studies including endpoints of endocrine adversity are lacking. The present study investigated the potential endocrine-disrupting effects of BP-3 in zebrafish (Danio rerio) in the Fish Sexual Development Test (Organisation for Economic Co-operation and Development TG 234) and a 12 day adult male zebrafish study. In TG 234, exposure from 0 to 60 d posthatch caused a monotone dose-dependent skewing of the phenotypic sex ratio towards less males and more female zebrafish (no observed effect concentration [NOEC]: 191 µg/L, lowest observed effect concentration [LOEC]: 388 µg/L). Besides, gonad maturation was affected in both female fish (NOEC 191 µg/L, LOEC 388 µg/L) and male fish (NOEC 388 µg/L, LOEC 470 µg/L). Exposure to BP-3 did not affect the vitellogenin concentration in TG 234. After 12 d exposure of adult male zebrafish, a slight but yet significant increase in the vitellogenin concentration was observed at 268 µg/L but not at 63 µg/L and 437 µg/l BP-3. Skewing of the sex ratio is a marker of an endocrine mediated mechanism as well as a marker of adversity and therefore the conclusion of the investigation is that BP-3 is an endocrine-disrupting chemical in accordance with the World Health Organization's definition.",
keywords = "Endocrine disrupters, UV filters, Vitellogenin, Sex ratio, Aquatic toxicology",
author = "Kinnberg, {Karin Lund} and Petersen, {Gitte I.} and Mette Albrektsen and Mite Minghlani and Awad, {Suad Mohamud} and Holbech, {Bente Frost} and Green, {John W.} and Poul Bjerregaard and Henrik Holbech",
year = "2015",
doi = "10.1002/etc.3129",
language = "English",
volume = "34",
pages = "2833--2840",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "JohnWiley & Sons, Inc.",
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Endocrine-disrupting effect of the ultraviolet filter benzophenone-3 in zebrafish, Danio rerio. / Kinnberg, Karin Lund; Petersen, Gitte I.; Albrektsen, Mette; Minghlani, Mite; Awad, Suad Mohamud; Holbech, Bente Frost; Green, John W.; Bjerregaard, Poul; Holbech, Henrik.

I: Environmental Toxicology and Chemistry, Bind 34, Nr. 12, 2015, s. 2833-2840.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Endocrine-disrupting effect of the ultraviolet filter benzophenone-3 in zebrafish, Danio rerio

AU - Kinnberg, Karin Lund

AU - Petersen, Gitte I.

AU - Albrektsen, Mette

AU - Minghlani, Mite

AU - Awad, Suad Mohamud

AU - Holbech, Bente Frost

AU - Green, John W.

AU - Bjerregaard, Poul

AU - Holbech, Henrik

PY - 2015

Y1 - 2015

N2 - The chemical ultraviolet (UV) filter benzophenone-3 (BP-3) is suspected to be an endocrine disruptor based on results from in vitro and in vivo testing. However, studies including endpoints of endocrine adversity are lacking. The present study investigated the potential endocrine-disrupting effects of BP-3 in zebrafish (Danio rerio) in the Fish Sexual Development Test (Organisation for Economic Co-operation and Development TG 234) and a 12 day adult male zebrafish study. In TG 234, exposure from 0 to 60 d posthatch caused a monotone dose-dependent skewing of the phenotypic sex ratio towards less males and more female zebrafish (no observed effect concentration [NOEC]: 191 µg/L, lowest observed effect concentration [LOEC]: 388 µg/L). Besides, gonad maturation was affected in both female fish (NOEC 191 µg/L, LOEC 388 µg/L) and male fish (NOEC 388 µg/L, LOEC 470 µg/L). Exposure to BP-3 did not affect the vitellogenin concentration in TG 234. After 12 d exposure of adult male zebrafish, a slight but yet significant increase in the vitellogenin concentration was observed at 268 µg/L but not at 63 µg/L and 437 µg/l BP-3. Skewing of the sex ratio is a marker of an endocrine mediated mechanism as well as a marker of adversity and therefore the conclusion of the investigation is that BP-3 is an endocrine-disrupting chemical in accordance with the World Health Organization's definition.

AB - The chemical ultraviolet (UV) filter benzophenone-3 (BP-3) is suspected to be an endocrine disruptor based on results from in vitro and in vivo testing. However, studies including endpoints of endocrine adversity are lacking. The present study investigated the potential endocrine-disrupting effects of BP-3 in zebrafish (Danio rerio) in the Fish Sexual Development Test (Organisation for Economic Co-operation and Development TG 234) and a 12 day adult male zebrafish study. In TG 234, exposure from 0 to 60 d posthatch caused a monotone dose-dependent skewing of the phenotypic sex ratio towards less males and more female zebrafish (no observed effect concentration [NOEC]: 191 µg/L, lowest observed effect concentration [LOEC]: 388 µg/L). Besides, gonad maturation was affected in both female fish (NOEC 191 µg/L, LOEC 388 µg/L) and male fish (NOEC 388 µg/L, LOEC 470 µg/L). Exposure to BP-3 did not affect the vitellogenin concentration in TG 234. After 12 d exposure of adult male zebrafish, a slight but yet significant increase in the vitellogenin concentration was observed at 268 µg/L but not at 63 µg/L and 437 µg/l BP-3. Skewing of the sex ratio is a marker of an endocrine mediated mechanism as well as a marker of adversity and therefore the conclusion of the investigation is that BP-3 is an endocrine-disrupting chemical in accordance with the World Health Organization's definition.

KW - Endocrine disrupters

KW - UV filters

KW - Vitellogenin

KW - Sex ratio

KW - Aquatic toxicology

UR - http://onlinelibrary.wiley.com/doi/10.1002/etc.3129/abstract

U2 - 10.1002/etc.3129

DO - 10.1002/etc.3129

M3 - Journal article

C2 - 26118430

VL - 34

SP - 2833

EP - 2840

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 12

ER -