Embryonic stem cells produce neurotrophins in response to cerebral tissue extract: Cell line-dependent differences

Kristine Bentz, Marek Molcanyi, Peter Riess, Andrea Elbers, Esther Pohl, Agapios Sachinidis, Jürgen Hescheler, Edmund Neugebauer, Ute Schäfer

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

Udgivelsesdato: 2007-Apr
OriginalsprogEngelsk
TidsskriftJournal of Neuroscience Research
Vol/bind85
Udgave nummer5
Sider (fra-til)1057-64
Antal sider7
ISSN0360-4012
DOI
StatusUdgivet - 1. apr. 2007
Udgivet eksterntJa

Fingeraftryk

Cell Line
Brain-Derived Neurotrophic Factor
Feeder Cells
Nerve Growth Factor
Neurotrophin 3
Percussion

Citer dette

Bentz, Kristine ; Molcanyi, Marek ; Riess, Peter ; Elbers, Andrea ; Pohl, Esther ; Sachinidis, Agapios ; Hescheler, Jürgen ; Neugebauer, Edmund ; Schäfer, Ute. / Embryonic stem cells produce neurotrophins in response to cerebral tissue extract: Cell line-dependent differences. I: Journal of Neuroscience Research. 2007 ; Bind 85, Nr. 5. s. 1057-64.
@article{46351480707211de9c46000ea68e967b,
title = "Embryonic stem cells produce neurotrophins in response to cerebral tissue extract: Cell line-dependent differences",
abstract = "In the present study, we compare the capacity of two different embryonic stem (ES) cell lines to secrete neurotrophins in response to cerebral tissue extract derived from healthy or injured rat brains. The intrinsic capacity of the embryonic cell lines BAC7 (feeder cell-dependent cultivation) to release brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) exceeded the release of these factors by CGR8 cells (feeder cell-free growth) by factors of 10 and 4, respectively. Nerve growth factor (NGF) was secreted only by BAC7 cells. Conditioning of cell lines with cerebral tissue extract derived from healthy or fluid percussion-injured rat brains resulted in a significant time-dependent increase in BDNF release in both cell lines. The increase in BDNF release by BAC7 cells was more pronounced when cells were incubated with brain extract derived from injured brain. However, differences in neurotrophin release associated with the origin of brain extract were at no time statistically significant. Neutrophin-3 and NGF release was inhibited when cell lines were exposed to cerebral tissue extract. The magnitude of the response to cerebral tissue extract was dependent on the intrinsic capacity of the cell lines to release neurotrophins. Our results clearly demonstrate significant variations in the intrinsic capability of different stem cell lines to produce neurotrophic factors. Furthermore, a significant modulation of neurotrophic factor release was observed following conditioning of cell lines with tissue extract derived from rat brains. A significant modulation of neurotrophin release dependent on the source of cerebral tissue extract used was not observed.",
author = "Kristine Bentz and Marek Molcanyi and Peter Riess and Andrea Elbers and Esther Pohl and Agapios Sachinidis and J{\"u}rgen Hescheler and Edmund Neugebauer and Ute Sch{\"a}fer",
note = "(c) 2007 Wiley-Liss, Inc.",
year = "2007",
month = "4",
day = "1",
doi = "10.1002/jnr.21219",
language = "English",
volume = "85",
pages = "1057--64",
journal = "Journal of Neuroscience Research",
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Bentz, K, Molcanyi, M, Riess, P, Elbers, A, Pohl, E, Sachinidis, A, Hescheler, J, Neugebauer, E & Schäfer, U 2007, 'Embryonic stem cells produce neurotrophins in response to cerebral tissue extract: Cell line-dependent differences', Journal of Neuroscience Research, bind 85, nr. 5, s. 1057-64. https://doi.org/10.1002/jnr.21219

Embryonic stem cells produce neurotrophins in response to cerebral tissue extract: Cell line-dependent differences. / Bentz, Kristine; Molcanyi, Marek; Riess, Peter; Elbers, Andrea; Pohl, Esther; Sachinidis, Agapios; Hescheler, Jürgen; Neugebauer, Edmund; Schäfer, Ute.

I: Journal of Neuroscience Research, Bind 85, Nr. 5, 01.04.2007, s. 1057-64.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Embryonic stem cells produce neurotrophins in response to cerebral tissue extract: Cell line-dependent differences

AU - Bentz, Kristine

AU - Molcanyi, Marek

AU - Riess, Peter

AU - Elbers, Andrea

AU - Pohl, Esther

AU - Sachinidis, Agapios

AU - Hescheler, Jürgen

AU - Neugebauer, Edmund

AU - Schäfer, Ute

N1 - (c) 2007 Wiley-Liss, Inc.

PY - 2007/4/1

Y1 - 2007/4/1

N2 - In the present study, we compare the capacity of two different embryonic stem (ES) cell lines to secrete neurotrophins in response to cerebral tissue extract derived from healthy or injured rat brains. The intrinsic capacity of the embryonic cell lines BAC7 (feeder cell-dependent cultivation) to release brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) exceeded the release of these factors by CGR8 cells (feeder cell-free growth) by factors of 10 and 4, respectively. Nerve growth factor (NGF) was secreted only by BAC7 cells. Conditioning of cell lines with cerebral tissue extract derived from healthy or fluid percussion-injured rat brains resulted in a significant time-dependent increase in BDNF release in both cell lines. The increase in BDNF release by BAC7 cells was more pronounced when cells were incubated with brain extract derived from injured brain. However, differences in neurotrophin release associated with the origin of brain extract were at no time statistically significant. Neutrophin-3 and NGF release was inhibited when cell lines were exposed to cerebral tissue extract. The magnitude of the response to cerebral tissue extract was dependent on the intrinsic capacity of the cell lines to release neurotrophins. Our results clearly demonstrate significant variations in the intrinsic capability of different stem cell lines to produce neurotrophic factors. Furthermore, a significant modulation of neurotrophic factor release was observed following conditioning of cell lines with tissue extract derived from rat brains. A significant modulation of neurotrophin release dependent on the source of cerebral tissue extract used was not observed.

AB - In the present study, we compare the capacity of two different embryonic stem (ES) cell lines to secrete neurotrophins in response to cerebral tissue extract derived from healthy or injured rat brains. The intrinsic capacity of the embryonic cell lines BAC7 (feeder cell-dependent cultivation) to release brain-derived neurotrophic factor (BDNF) or neurotrophin-3 (NT-3) exceeded the release of these factors by CGR8 cells (feeder cell-free growth) by factors of 10 and 4, respectively. Nerve growth factor (NGF) was secreted only by BAC7 cells. Conditioning of cell lines with cerebral tissue extract derived from healthy or fluid percussion-injured rat brains resulted in a significant time-dependent increase in BDNF release in both cell lines. The increase in BDNF release by BAC7 cells was more pronounced when cells were incubated with brain extract derived from injured brain. However, differences in neurotrophin release associated with the origin of brain extract were at no time statistically significant. Neutrophin-3 and NGF release was inhibited when cell lines were exposed to cerebral tissue extract. The magnitude of the response to cerebral tissue extract was dependent on the intrinsic capacity of the cell lines to release neurotrophins. Our results clearly demonstrate significant variations in the intrinsic capability of different stem cell lines to produce neurotrophic factors. Furthermore, a significant modulation of neurotrophic factor release was observed following conditioning of cell lines with tissue extract derived from rat brains. A significant modulation of neurotrophin release dependent on the source of cerebral tissue extract used was not observed.

U2 - 10.1002/jnr.21219

DO - 10.1002/jnr.21219

M3 - Journal article

VL - 85

SP - 1057

EP - 1064

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 5

ER -