Various elements (K, Fe, Cu, Zn, Br) were measured by X-ray flourescence spectrometry in cellular and connective tissue fractions of normal and cirrhotic liver samples obtained at autopsy. Normal livers: 32 subjects (16 males, 16 females) median age 69 years. Cirrhotic livers: 14 subjects (13 males, 1 female) with alcoholic cirrhosis, median age 55 years. Cellular and connective tissue fractions were separated by blending and sieving of the liver samples. Normal livers:K, Cu and Zn content was higher, and Br content lower in the cellular than in the connective tissue fractions. Median values in cellular/connective tissue fractions in mmol/kg/dry weight were: K, 121.04/48.67, p<0.0001; Fe, 16.12/13.95, NS; Cu, 0.326/0.223, p<0.001; Zn, 3.20/2.78, p<0.02; Br, 0.0838/0.1827, p<0.0001. Cirrhotic livers: K content was higher and Br content lower in cellular than in connective tissue fractions, while Fe, Cu and Zn displayed no difference. K, 122.42/22.58, p<0.0001; Fe, 13.00/16.58, NS; Cu, 0.444/0.595, NS; Zn, 2.19/2.07, NS; Br, 0.1602/0.2340, p<0.001. In cellular fractions of cirrhotic livers, K and Fe content was within the normal range, Zn content lower (p<0.03), and Cu and Br content higher (p<0.01, p<0.04) than normal. In connective tissue fractions of cirrhotic livers, Fe content was within normal range, K and Zn content lower (p<0.0001, p<0.03), and Cu and Br content higher (p<0.01, p<0.001) than normal. From a practical point of view, when assessing element content in cirrhotic livers, it does not seem necessary to correct for the connective tissue content.
|Tidsskrift||Trace Elements in Medicine|
|Status||Udgivet - 1990|
Laursen, J., Milman, N., Leth, P. M., & Asnæs, S. (1990). Elements in normal and cirrhotic human liver. Potassium, iron, copper, zinc and bromine measured by X-ray fluorescence spectrometry. Trace Elements in Medicine, 7(3), 118-123.