Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions

Ruth Montes, Hasna Ahyayauch, Maitane Ibarguren, Jesus Sot, Alicia Alonso, Luis Bagatolli, Felix Goni

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

Resumé

Giant unilamellar vesicles (GUVs) constitute a cell-sized model membrane system that allows direct visualization of particular membrane-related phenomena, such as domain formation, at the level of single vesicles using fluorescence microscopy-related techniques. Currently available protocols for the preparation of GUVs work only at very low salt concentrations, thus precluding experimentation under physiological conditions. In addition, the GUVs thus obtained lack membrane compositional asymmetry. Here we
show how to prepare GUVs using a new protocol based on the electroformation method either from native membranes or organic lipid mixtures at physiological ionic strength. Additionally, we describe methods to test whether membrane proteins and glycosphingolipids preserve their natural orientation after electroformation of GUVs composed of native membranes
OriginalsprogEngelsk
TitelLiposomes: Methods and Protocols, Volumen 2: Biological Membrane Models, Methods in Molecular Biology
RedaktørerVolkmar Weissig
Antal sider10
Vol/bind606
ForlagHumana Press
Publikationsdato2010
Sider105-114
Kapitel9
ISBN (Trykt)978-1-60761-446-3
StatusUdgivet - 2010

Fingeraftryk

Unilamellar Liposomes
Ionic strength
Membranes
Lipids
Glycosphingolipids
Fluorescence microscopy
Membrane Proteins
Visualization
Salts

Citer dette

Montes, R., Ahyayauch, H., Ibarguren, M., Sot, J., Alonso, A., Bagatolli, L., & Goni, F. (2010). Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions. I V. Weissig (red.), Liposomes: Methods and Protocols, Volumen 2: Biological Membrane Models, Methods in Molecular Biology (Bind 606, s. 105-114). Humana Press.
Montes, Ruth ; Ahyayauch, Hasna ; Ibarguren, Maitane ; Sot, Jesus ; Alonso, Alicia ; Bagatolli, Luis ; Goni, Felix. / Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions. Liposomes: Methods and Protocols, Volumen 2: Biological Membrane Models, Methods in Molecular Biology. red. / Volkmar Weissig. Bind 606 Humana Press, 2010. s. 105-114
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abstract = "Giant unilamellar vesicles (GUVs) constitute a cell-sized model membrane system that allows direct visualization of particular membrane-related phenomena, such as domain formation, at the level of single vesicles using fluorescence microscopy-related techniques. Currently available protocols for the preparation of GUVs work only at very low salt concentrations, thus precluding experimentation under physiological conditions. In addition, the GUVs thus obtained lack membrane compositional asymmetry. Here weshow how to prepare GUVs using a new protocol based on the electroformation method either from native membranes or organic lipid mixtures at physiological ionic strength. Additionally, we describe methods to test whether membrane proteins and glycosphingolipids preserve their natural orientation after electroformation of GUVs composed of native membranes",
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Montes, R, Ahyayauch, H, Ibarguren, M, Sot, J, Alonso, A, Bagatolli, L & Goni, F 2010, Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions. i V Weissig (red.), Liposomes: Methods and Protocols, Volumen 2: Biological Membrane Models, Methods in Molecular Biology. bind 606, Humana Press, s. 105-114.

Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions. / Montes, Ruth; Ahyayauch, Hasna; Ibarguren, Maitane ; Sot, Jesus; Alonso, Alicia; Bagatolli, Luis; Goni, Felix.

Liposomes: Methods and Protocols, Volumen 2: Biological Membrane Models, Methods in Molecular Biology. red. / Volkmar Weissig. Bind 606 Humana Press, 2010. s. 105-114.

Publikation: Bidrag til bog/antologi/rapport/konference-proceedingBidrag til bog/antologiForskningpeer review

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T1 - Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions

AU - Montes, Ruth

AU - Ahyayauch, Hasna

AU - Ibarguren, Maitane

AU - Sot, Jesus

AU - Alonso, Alicia

AU - Bagatolli, Luis

AU - Goni, Felix

PY - 2010

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N2 - Giant unilamellar vesicles (GUVs) constitute a cell-sized model membrane system that allows direct visualization of particular membrane-related phenomena, such as domain formation, at the level of single vesicles using fluorescence microscopy-related techniques. Currently available protocols for the preparation of GUVs work only at very low salt concentrations, thus precluding experimentation under physiological conditions. In addition, the GUVs thus obtained lack membrane compositional asymmetry. Here weshow how to prepare GUVs using a new protocol based on the electroformation method either from native membranes or organic lipid mixtures at physiological ionic strength. Additionally, we describe methods to test whether membrane proteins and glycosphingolipids preserve their natural orientation after electroformation of GUVs composed of native membranes

AB - Giant unilamellar vesicles (GUVs) constitute a cell-sized model membrane system that allows direct visualization of particular membrane-related phenomena, such as domain formation, at the level of single vesicles using fluorescence microscopy-related techniques. Currently available protocols for the preparation of GUVs work only at very low salt concentrations, thus precluding experimentation under physiological conditions. In addition, the GUVs thus obtained lack membrane compositional asymmetry. Here weshow how to prepare GUVs using a new protocol based on the electroformation method either from native membranes or organic lipid mixtures at physiological ionic strength. Additionally, we describe methods to test whether membrane proteins and glycosphingolipids preserve their natural orientation after electroformation of GUVs composed of native membranes

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Montes R, Ahyayauch H, Ibarguren M, Sot J, Alonso A, Bagatolli L et al. Electroformation of Giant Unilamellar Vesicles from Native Membranes and Organic Lipid Mixtures for the Study of Lipid Domains under Physiological Ionic-Strength Conditions. I Weissig V, red., Liposomes: Methods and Protocols, Volumen 2: Biological Membrane Models, Methods in Molecular Biology. Bind 606. Humana Press. 2010. s. 105-114