Elastin organization in pig and cardiovascular disease patients' pericardial resistance arteries

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Resumé

Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass grafting or cardiac valve replacement surgeries, can serve as a source of resistance arteries for structural research in cardiovascular disease patients. We applied two-photon excitation fluorescence microscopy to study the parietal pericardium and isolated pericardial resistance arteries with a focus on the collagen and elastin components of the extracellular matrix. Initial findings in pig tissue were confirmed in patient biopsies. The microarchitecture of the internal elastic lamina in both the pig and patient pericardial resistance arteries (studied at a transmural pressure of 100 mm Hg) is fiber like, and no prominent external elastic lamina could be observed. This microarchitecture is very different from that in rat mesenteric arteries frequently used for resistance artery research. In conclusion, we add three-dimensional information on the structure of the extracellular matrix in resistance arteries from cardiovascular disease patients and propose further use of patient pericardial resistance arteries for studies of the human microvasculature. © 2015 S. Karger AG, Basel.
OriginalsprogEngelsk
TidsskriftJournal of Vascular Research
Vol/bind52
Udgave nummer1
Antal sider11
ISSN1018-1172
DOI
StatusUdgivet - 2015

Fingeraftryk

Pericardium
Heart Valves
Photons
Fluorescence Microscopy
Research
Essential Hypertension

Citer dette

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title = "Elastin organization in pig and cardiovascular disease patients' pericardial resistance arteries",
abstract = "Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass grafting or cardiac valve replacement surgeries, can serve as a source of resistance arteries for structural research in cardiovascular disease patients. We applied two-photon excitation fluorescence microscopy to study the parietal pericardium and isolated pericardial resistance arteries with a focus on the collagen and elastin components of the extracellular matrix. Initial findings in pig tissue were confirmed in patient biopsies. The microarchitecture of the internal elastic lamina in both the pig and patient pericardial resistance arteries (studied at a transmural pressure of 100 mm Hg) is fiber like, and no prominent external elastic lamina could be observed. This microarchitecture is very different from that in rat mesenteric arteries frequently used for resistance artery research. In conclusion, we add three-dimensional information on the structure of the extracellular matrix in resistance arteries from cardiovascular disease patients and propose further use of patient pericardial resistance arteries for studies of the human microvasculature. {\circledC} 2015 S. Karger AG, Basel.",
keywords = "Elastin, Collagen, Remodelling, Extracellular Matrix, Two-photon excitation fluorescence microscopy",
author = "Maria Bloksgaard and Thomas Leurgans and Inger Nissen and Jensen, {Pia S{\o}ndergaard} and Hansen, {Maria Lyck} and Brewer, {Jonathan R.} and Luis Bagatolli and Niels Marcussen and Akhmadjon Irmukhamedov and Rasmussen, {Lars Melholt} and {De Mey}, {Jo G. R}",
year = "2015",
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language = "English",
volume = "52",
journal = "Journal of Vascular Research",
issn = "1018-1172",
publisher = "S. Karger AG",
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TY - JOUR

T1 - Elastin organization in pig and cardiovascular disease patients' pericardial resistance arteries

AU - Bloksgaard, Maria

AU - Leurgans, Thomas

AU - Nissen, Inger

AU - Jensen, Pia Søndergaard

AU - Hansen, Maria Lyck

AU - Brewer, Jonathan R.

AU - Bagatolli, Luis

AU - Marcussen, Niels

AU - Irmukhamedov, Akhmadjon

AU - Rasmussen, Lars Melholt

AU - De Mey, Jo G. R

PY - 2015

Y1 - 2015

N2 - Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass grafting or cardiac valve replacement surgeries, can serve as a source of resistance arteries for structural research in cardiovascular disease patients. We applied two-photon excitation fluorescence microscopy to study the parietal pericardium and isolated pericardial resistance arteries with a focus on the collagen and elastin components of the extracellular matrix. Initial findings in pig tissue were confirmed in patient biopsies. The microarchitecture of the internal elastic lamina in both the pig and patient pericardial resistance arteries (studied at a transmural pressure of 100 mm Hg) is fiber like, and no prominent external elastic lamina could be observed. This microarchitecture is very different from that in rat mesenteric arteries frequently used for resistance artery research. In conclusion, we add three-dimensional information on the structure of the extracellular matrix in resistance arteries from cardiovascular disease patients and propose further use of patient pericardial resistance arteries for studies of the human microvasculature. © 2015 S. Karger AG, Basel.

AB - Peripheral vascular resistance is increased in essential hypertension. This involves structural changes of resistance arteries and stiffening of the arterial wall, including remodeling of the extracellular matrix. We hypothesized that biopsies of the human parietal pericardium, obtained during coronary artery bypass grafting or cardiac valve replacement surgeries, can serve as a source of resistance arteries for structural research in cardiovascular disease patients. We applied two-photon excitation fluorescence microscopy to study the parietal pericardium and isolated pericardial resistance arteries with a focus on the collagen and elastin components of the extracellular matrix. Initial findings in pig tissue were confirmed in patient biopsies. The microarchitecture of the internal elastic lamina in both the pig and patient pericardial resistance arteries (studied at a transmural pressure of 100 mm Hg) is fiber like, and no prominent external elastic lamina could be observed. This microarchitecture is very different from that in rat mesenteric arteries frequently used for resistance artery research. In conclusion, we add three-dimensional information on the structure of the extracellular matrix in resistance arteries from cardiovascular disease patients and propose further use of patient pericardial resistance arteries for studies of the human microvasculature. © 2015 S. Karger AG, Basel.

KW - Elastin

KW - Collagen

KW - Remodelling

KW - Extracellular Matrix

KW - Two-photon excitation fluorescence microscopy

U2 - 10.1159/000376548

DO - 10.1159/000376548

M3 - Journal article

VL - 52

JO - Journal of Vascular Research

JF - Journal of Vascular Research

SN - 1018-1172

IS - 1

ER -