Effect of detergents on the physico-chemical properties of skin stratum corneum: A two-photon excitation fluorescence microscopy study.

Bidragets oversatte titel: Effekt af detergenter på de fysisk-kemiske egenskaber af hudens hornlag - et to-fotons excitations mikroskopi studie

Maria Bloksgaard, Jonathan R. Brewer, Eugene Pashkovski, KP Ananthapadmanabhan, Jens Ahm Sørensen, Luis Bagatolli

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

OBJECTIVE:

Understanding the structural and dynamical features of skin is critical for advancing innovation in personal care and drug discovery. Synthetic detergent mixtures used in commercially available body wash products are thought to be less aggressive towards the skin barrier when compared to conventional detergents. The aim of this work is to comparatively characterize the effect of a mild synthetic cleanser mixture (SCM) and sodium dodecyl sulphate (SDS) on the hydration state of the intercellular lipid matrix and on proton activity of excised skin stratum corneum (SC).
METHOD:

Experiments were performed using two-photon excitation fluorescence microscopy. Fluorescent images of fluorescence reporters sensitive to proton activity and hydration of SC were obtained in excised skin and examined in presence and absence of SCM and SDS detergents.
RESULTS:

Hydration of the intercellular lipid matrix to a depth of 10μm into the SC was increased upon treatment with SCM, whereas SDS shows this effect only at the very surface of SC. The proton activity of SC remained unaffected by treatment with either detergent.
CONCLUSION:

While our study indicates that the SC is very resistant to external stimuli, it also shows that, in contrast to the response to SDS, SCM to some extent modulates the in-depth hydration properties of the intercellular lipid matrix within excised skin SC.
OriginalsprogEngelsk
TidsskriftInternational Journal of Cosmetic Science
Vol/bind36
Udgave nummer1
Sider (fra-til)39-45
ISSN0142-5463
DOI
StatusUdgivet - 2014

Citer dette

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title = "Effect of detergents on the physico-chemical properties of skin stratum corneum: A two-photon excitation fluorescence microscopy study.",
abstract = "OBJECTIVE:Understanding the structural and dynamical features of skin is critical for advancing innovation in personal care and drug discovery. Synthetic detergent mixtures used in commercially available body wash products are thought to be less aggressive towards the skin barrier when compared to conventional detergents. The aim of this work is to comparatively characterize the effect of a mild synthetic cleanser mixture (SCM) and sodium dodecyl sulphate (SDS) on the hydration state of the intercellular lipid matrix and on proton activity of excised skin stratum corneum (SC).METHOD:Experiments were performed using two-photon excitation fluorescence microscopy. Fluorescent images of fluorescence reporters sensitive to proton activity and hydration of SC were obtained in excised skin and examined in presence and absence of SCM and SDS detergents.RESULTS:Hydration of the intercellular lipid matrix to a depth of 10μm into the SC was increased upon treatment with SCM, whereas SDS shows this effect only at the very surface of SC. The proton activity of SC remained unaffected by treatment with either detergent.CONCLUSION:While our study indicates that the SC is very resistant to external stimuli, it also shows that, in contrast to the response to SDS, SCM to some extent modulates the in-depth hydration properties of the intercellular lipid matrix within excised skin SC.",
author = "Maria Bloksgaard and Brewer, {Jonathan R.} and Eugene Pashkovski and KP Ananthapadmanabhan and S{\o}rensen, {Jens Ahm} and Luis Bagatolli",
year = "2014",
doi = "10.1111/ics.12089",
language = "English",
volume = "36",
pages = "39--45",
journal = "International Journal of Cosmetic Science",
issn = "0142-5463",
publisher = "Wiley-Blackwell",
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}

Effect of detergents on the physico-chemical properties of skin stratum corneum: A two-photon excitation fluorescence microscopy study. / Bloksgaard, Maria; Brewer, Jonathan R.; Pashkovski, Eugene; Ananthapadmanabhan, KP; Sørensen, Jens Ahm; Bagatolli, Luis.

I: International Journal of Cosmetic Science, Bind 36, Nr. 1, 2014, s. 39-45.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Effect of detergents on the physico-chemical properties of skin stratum corneum: A two-photon excitation fluorescence microscopy study.

AU - Bloksgaard, Maria

AU - Brewer, Jonathan R.

AU - Pashkovski, Eugene

AU - Ananthapadmanabhan, KP

AU - Sørensen, Jens Ahm

AU - Bagatolli, Luis

PY - 2014

Y1 - 2014

N2 - OBJECTIVE:Understanding the structural and dynamical features of skin is critical for advancing innovation in personal care and drug discovery. Synthetic detergent mixtures used in commercially available body wash products are thought to be less aggressive towards the skin barrier when compared to conventional detergents. The aim of this work is to comparatively characterize the effect of a mild synthetic cleanser mixture (SCM) and sodium dodecyl sulphate (SDS) on the hydration state of the intercellular lipid matrix and on proton activity of excised skin stratum corneum (SC).METHOD:Experiments were performed using two-photon excitation fluorescence microscopy. Fluorescent images of fluorescence reporters sensitive to proton activity and hydration of SC were obtained in excised skin and examined in presence and absence of SCM and SDS detergents.RESULTS:Hydration of the intercellular lipid matrix to a depth of 10μm into the SC was increased upon treatment with SCM, whereas SDS shows this effect only at the very surface of SC. The proton activity of SC remained unaffected by treatment with either detergent.CONCLUSION:While our study indicates that the SC is very resistant to external stimuli, it also shows that, in contrast to the response to SDS, SCM to some extent modulates the in-depth hydration properties of the intercellular lipid matrix within excised skin SC.

AB - OBJECTIVE:Understanding the structural and dynamical features of skin is critical for advancing innovation in personal care and drug discovery. Synthetic detergent mixtures used in commercially available body wash products are thought to be less aggressive towards the skin barrier when compared to conventional detergents. The aim of this work is to comparatively characterize the effect of a mild synthetic cleanser mixture (SCM) and sodium dodecyl sulphate (SDS) on the hydration state of the intercellular lipid matrix and on proton activity of excised skin stratum corneum (SC).METHOD:Experiments were performed using two-photon excitation fluorescence microscopy. Fluorescent images of fluorescence reporters sensitive to proton activity and hydration of SC were obtained in excised skin and examined in presence and absence of SCM and SDS detergents.RESULTS:Hydration of the intercellular lipid matrix to a depth of 10μm into the SC was increased upon treatment with SCM, whereas SDS shows this effect only at the very surface of SC. The proton activity of SC remained unaffected by treatment with either detergent.CONCLUSION:While our study indicates that the SC is very resistant to external stimuli, it also shows that, in contrast to the response to SDS, SCM to some extent modulates the in-depth hydration properties of the intercellular lipid matrix within excised skin SC.

U2 - 10.1111/ics.12089

DO - 10.1111/ics.12089

M3 - Journal article

VL - 36

SP - 39

EP - 45

JO - International Journal of Cosmetic Science

JF - International Journal of Cosmetic Science

SN - 0142-5463

IS - 1

ER -