Effect of storage on osteoinductive properties of demineralized bone in rats

E M Pinholt, E Solheim

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

A requirement for the clinical use of demineralized bone is the possibility of storing the material without loss of its osteoinductive properties. Seventy-five 8-week-old male Wistar rats were randomly assigned to one of five groups of 15 rats each. Lyophilized demineralized allogeneic bone was prepared and implanted in the abdominal muscle either without prior storage (control group) or after storage for 9 or 14 months at -70 degrees C or 4 degrees C (four experimental groups). Bone formation in the implants was evaluated quantitatively 4 weeks postoperatively by measuring the strontium 85 uptake of the recovered implants. Storage for 9 months at both temperatures did not affect the osteoinduction, whereas storage for 14 months at both temperatures led to a statistically significant decrease in osteoinduction.
OriginalsprogEngelsk
TidsskriftAnnals of Plastic Surgery
Vol/bind33
Udgave nummer5
Sider (fra-til)531-5
Antal sider5
ISSN0148-7043
StatusUdgivet - 1. nov. 1994
Udgivet eksterntJa

Fingeraftryk

Abdominal Muscles
Osteogenesis
Wistar Rats
Control Groups

Citer dette

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Effect of storage on osteoinductive properties of demineralized bone in rats. / Pinholt, E M; Solheim, E.

I: Annals of Plastic Surgery, Bind 33, Nr. 5, 01.11.1994, s. 531-5.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Effect of storage on osteoinductive properties of demineralized bone in rats

AU - Pinholt, E M

AU - Solheim, E

PY - 1994/11/1

Y1 - 1994/11/1

N2 - A requirement for the clinical use of demineralized bone is the possibility of storing the material without loss of its osteoinductive properties. Seventy-five 8-week-old male Wistar rats were randomly assigned to one of five groups of 15 rats each. Lyophilized demineralized allogeneic bone was prepared and implanted in the abdominal muscle either without prior storage (control group) or after storage for 9 or 14 months at -70 degrees C or 4 degrees C (four experimental groups). Bone formation in the implants was evaluated quantitatively 4 weeks postoperatively by measuring the strontium 85 uptake of the recovered implants. Storage for 9 months at both temperatures did not affect the osteoinduction, whereas storage for 14 months at both temperatures led to a statistically significant decrease in osteoinduction.

AB - A requirement for the clinical use of demineralized bone is the possibility of storing the material without loss of its osteoinductive properties. Seventy-five 8-week-old male Wistar rats were randomly assigned to one of five groups of 15 rats each. Lyophilized demineralized allogeneic bone was prepared and implanted in the abdominal muscle either without prior storage (control group) or after storage for 9 or 14 months at -70 degrees C or 4 degrees C (four experimental groups). Bone formation in the implants was evaluated quantitatively 4 weeks postoperatively by measuring the strontium 85 uptake of the recovered implants. Storage for 9 months at both temperatures did not affect the osteoinduction, whereas storage for 14 months at both temperatures led to a statistically significant decrease in osteoinduction.

M3 - Journal article

C2 - 7857048

VL - 33

SP - 531

EP - 535

JO - Annals of Plastic Surgery

JF - Annals of Plastic Surgery

SN - 0148-7043

IS - 5

ER -