Effect of locked nucleic acid (LNA) modification on hybridization kinetics of DNA duplex

Amit Arora, Harleen Kaur, Jesper Wengel, Souvik Maiti

Publikation: Bidrag til tidsskriftKonferenceartikelForskningpeer review

Abstrakt

The effect of locked nucleic acid (LNA) modification on hybridization kinetics of DNA duplex formation has been studied at varying salt concentration (Na+ and Mg2+) using surface-plasmon resonance. The study suggested that the increased stability of LNA containing duplexes mainly originates from the slower dissociation rates constants of the duplexes. An increase in salt concentration increased the binding affinity of the individual duplexes by raising their association rate constants. Monitoring the change in binding affinity with respect to salt concentration revealed that of LNA-associated enhancement in helical stability mainly results from the changes in the non-electrostatic interactions upon duplex formation.

OriginalsprogEngelsk
BogserieNucleic Acids Symposium Series
Udgave nummer1
Sider (fra-til)417-418
Antal sider2
ISSN0261-3166
DOI
StatusUdgivet - 2008
BegivenhedEffect of locked nucleic acid (LNA) modification on hybridization kinetics of DNA duplex -
Varighed: 24. aug. 2010 → …

Konference

KonferenceEffect of locked nucleic acid (LNA) modification on hybridization kinetics of DNA duplex
Periode24/08/2010 → …

Bibliografisk note

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