Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro

Lijin Zou, Xuenong Zou, Li Chen, Haisheng Li, Tina Mygind, Moustapha Kassem, Cody Bünger

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

Resumé

Udgivelsesdato: 2008-May
OriginalsprogEngelsk
TidsskriftJournal of Orthopaedic Research
Vol/bind26
Udgave nummer5
Sider (fra-til)713-20
ISSN0736-0266
DOI
StatusUdgivet - 1. maj 2008

Fingeraftryk

Hyaluronic Acid
Mesenchymal Stromal Cells
Cell Proliferation
In Vitro Techniques
Osteocalcin
Collagen Type I
Morphogenesis
Cell Movement
Cell Differentiation
Up-Regulation
Polymerase Chain Reaction

Citer dette

Zou, Lijin ; Zou, Xuenong ; Chen, Li ; Li, Haisheng ; Mygind, Tina ; Kassem, Moustapha ; Bünger, Cody. / Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro. I: Journal of Orthopaedic Research. 2008 ; Bind 26, Nr. 5. s. 713-20.
@article{e9c0052038c211deb0ce000ea68e967b,
title = "Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro",
abstract = "Hyaluronan (HA) plays a predominant role in tissue morphogenesis, cell migration, proliferation, and cell differentiation. The aims of the present study were to investigate whether (i) prolonged presence of high concentration (4.0 mg/mL) 800 KDa HA and (ii) pretreatment with HA can modify osteogenic differentiation of pig bone marrow stromal cells (pBMSC). Cell proliferation and mineralization were measured. Expression of differentiation-related genes was evaluated by means of real-time reverse transcription polymerase chain reaction (RT-PCR). HA increased cell proliferation on day 7. HA decreased the basal level of bone-related gene expression and increased the basal level of sox9 marginally during 7-day pretreatment with HA. HA increased calcium deposit on day 21. cbfa1, ALP, and type 1 alpha collagen (Col1) expression was increased when pBMSC were cultivated in osteogenic medium, whereas their expression was decreased in the presence of HA on day 7. On day 14, the addition of HA upregulated cbfa1 and ALP expression compared to osteogenic medium group; there was no significant difference in Col1 expression. At day 21, osteocalcin (OC) expression showed 2.5-fold upregulation over osteogenic medium. These results suggest that exogenous HA stimulates endogenous HA, which together may play a synergetic role in osteogenic differentiation under osteoinducing conditions although gene expression was inhibited at the early stage.",
keywords = "Adjuvants, Immunologic, Animals, Ascorbic Acid, Bone Marrow Cells, Calcification, Physiologic, Cell Differentiation, Cell Proliferation, Cells, Cultured, Dexamethasone, Female, Gene Expression, Glucocorticoids, Glucuronosyltransferase, Glycerophosphates, Hyaluronic Acid, Osteogenesis, RNA, Messenger, Swine, Vitamins",
author = "Lijin Zou and Xuenong Zou and Li Chen and Haisheng Li and Tina Mygind and Moustapha Kassem and Cody B{\"u}nger",
note = "(c) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.",
year = "2008",
month = "5",
day = "1",
doi = "10.1002/jor.20539",
language = "English",
volume = "26",
pages = "713--20",
journal = "Journal of Orthopaedic Research",
issn = "0736-0266",
publisher = "JohnWiley & Sons, Inc.",
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}

Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro. / Zou, Lijin; Zou, Xuenong; Chen, Li; Li, Haisheng; Mygind, Tina; Kassem, Moustapha; Bünger, Cody.

I: Journal of Orthopaedic Research, Bind 26, Nr. 5, 01.05.2008, s. 713-20.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

TY - JOUR

T1 - Effect of hyaluronan on osteogenic differentiation of porcine bone marrow stromal cells in vitro

AU - Zou, Lijin

AU - Zou, Xuenong

AU - Chen, Li

AU - Li, Haisheng

AU - Mygind, Tina

AU - Kassem, Moustapha

AU - Bünger, Cody

N1 - (c) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.

PY - 2008/5/1

Y1 - 2008/5/1

N2 - Hyaluronan (HA) plays a predominant role in tissue morphogenesis, cell migration, proliferation, and cell differentiation. The aims of the present study were to investigate whether (i) prolonged presence of high concentration (4.0 mg/mL) 800 KDa HA and (ii) pretreatment with HA can modify osteogenic differentiation of pig bone marrow stromal cells (pBMSC). Cell proliferation and mineralization were measured. Expression of differentiation-related genes was evaluated by means of real-time reverse transcription polymerase chain reaction (RT-PCR). HA increased cell proliferation on day 7. HA decreased the basal level of bone-related gene expression and increased the basal level of sox9 marginally during 7-day pretreatment with HA. HA increased calcium deposit on day 21. cbfa1, ALP, and type 1 alpha collagen (Col1) expression was increased when pBMSC were cultivated in osteogenic medium, whereas their expression was decreased in the presence of HA on day 7. On day 14, the addition of HA upregulated cbfa1 and ALP expression compared to osteogenic medium group; there was no significant difference in Col1 expression. At day 21, osteocalcin (OC) expression showed 2.5-fold upregulation over osteogenic medium. These results suggest that exogenous HA stimulates endogenous HA, which together may play a synergetic role in osteogenic differentiation under osteoinducing conditions although gene expression was inhibited at the early stage.

AB - Hyaluronan (HA) plays a predominant role in tissue morphogenesis, cell migration, proliferation, and cell differentiation. The aims of the present study were to investigate whether (i) prolonged presence of high concentration (4.0 mg/mL) 800 KDa HA and (ii) pretreatment with HA can modify osteogenic differentiation of pig bone marrow stromal cells (pBMSC). Cell proliferation and mineralization were measured. Expression of differentiation-related genes was evaluated by means of real-time reverse transcription polymerase chain reaction (RT-PCR). HA increased cell proliferation on day 7. HA decreased the basal level of bone-related gene expression and increased the basal level of sox9 marginally during 7-day pretreatment with HA. HA increased calcium deposit on day 21. cbfa1, ALP, and type 1 alpha collagen (Col1) expression was increased when pBMSC were cultivated in osteogenic medium, whereas their expression was decreased in the presence of HA on day 7. On day 14, the addition of HA upregulated cbfa1 and ALP expression compared to osteogenic medium group; there was no significant difference in Col1 expression. At day 21, osteocalcin (OC) expression showed 2.5-fold upregulation over osteogenic medium. These results suggest that exogenous HA stimulates endogenous HA, which together may play a synergetic role in osteogenic differentiation under osteoinducing conditions although gene expression was inhibited at the early stage.

KW - Adjuvants, Immunologic

KW - Animals

KW - Ascorbic Acid

KW - Bone Marrow Cells

KW - Calcification, Physiologic

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cells, Cultured

KW - Dexamethasone

KW - Female

KW - Gene Expression

KW - Glucocorticoids

KW - Glucuronosyltransferase

KW - Glycerophosphates

KW - Hyaluronic Acid

KW - Osteogenesis

KW - RNA, Messenger

KW - Swine

KW - Vitamins

U2 - 10.1002/jor.20539

DO - 10.1002/jor.20539

M3 - Journal article

VL - 26

SP - 713

EP - 720

JO - Journal of Orthopaedic Research

JF - Journal of Orthopaedic Research

SN - 0736-0266

IS - 5

ER -