@article{4d7f82307d1911de9c46000ea68e967b,
title = "Effect of heterologous expression of acyl-CoA-binding protein on acyl-CoA level and composition in yeast",
abstract = "We have expressed a bovine synthetic acyl-CoA-binding protein (ACBP) gene in yeast (Saccharomyces cerevisiae) under the control of the GAL1 promoter. The heterologously expressed bovine ACBP constituted up to 6.4% of total cellular protein and the processing was identical with that of native bovine ACBP, i.e. the initiating methionine was removed and the following serine residue was N-acetylated. The expression of this protein did not affect the growth rate of the cells. Determination of the yeast acyl-CoA pool size showed a close positive correlation between the ACBP content of the cells and the size of the acyl-CoA pool. Thus ACBP can act as an intracellular acyl-CoA pool former. Possible physiological functions of ACBP in cells are discussed.",
keywords = "Acyl Coenzyme A, Animals, Base Sequence, Carrier Proteins, Cattle, Cloning, Molecular, Escherichia coli, Fatty Acid-Binding Proteins, Molecular Sequence Data, Neoplasm Proteins, Oligonucleotides, Plasmids, Promoter Regions, Genetic, Recombinant Proteins, Saccharomyces cerevisiae",
author = "S Mandrup and R Jepsen and H Sk{\o}tt and J Rosendal and P H{\o}jrup and K Kristiansen and J Knudsen",
year = "1993",
month = mar,
day = "1",
doi = "10.1042/bj2900369",
language = "English",
volume = "290",
pages = "369--374",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd",
number = "2",
}