Effect of anaerobiosis on indigenous microorganisms in blackwater with fish offal as co-substrate

Ragnhildur Gunnarsdottir, Stefan Heiske, Pernille Erland Jensen, Jens Ejbye Schmidt, Arne Villumsen, Petter Deinboll Jenssen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Resumé

The aim of this study was to compare the effect of mesophilic anaerobic digestion with aerobic storage on the survival of selected indigenous microorganisms and microbial groups in blackwater, including the effect of addition of Greenlandic Halibut and shrimp offal. The methane yield of the different substrate mixtures was determined in batch experiments to study possible correlation between methanogenic activity in the anaerobic digesters and reduction of indigenous microorganisms in the blackwater. By the end of the experiments a recovery study was conducted to determine possible injury of the microorganisms. In both anaerobic and aerobic samples, survival of Escherichia coli was better in the presence of Greenlandic Halibut offal when compared to samples containing blackwater only and blackwater and shrimp offal, possibly due to more available carbon in the samples containing Greenlandic Halibut offal. Reduction of faecal streptococci was large under both anaerobic and aerobic conditions, and the results indicated a complete removal of faecal streptococci in the anaerobic samples containing blackwater and a mixture of blackwater and shrimp offal after 17 and 31 days, respectively. Amoxicillin resistant bacteria were reduced in the anaerobic samples in the beginning of the study but increased towards the end of it. The opposite pattern was observed in the aerobic samples, with a growth in the beginning followed by a reduction. During the anaerobic digestion tetracycline resistant bacteria showed the least reduction in the mixture of blackwater and shrimp offal, which had the lowest methane yield while the highest reduction was observed in the mixture of blackwater and Greenlandic Halibut, where the highest methane yield was measured Reduction of coliphages was larger under anaerobic conditions. Addition of fish offal had no effect on survival of coliphages. The results of the recovery study indicated that a fraction of the E. coli in the aerobic blackwater sample and of the faecal streptococci in both the anaerobic and aerobic samples containing blackwater and Greenlandic Halibut were injured only, and thus able to resuscitate during recovery.
OriginalsprogEngelsk
TidsskriftWater Research
Vol/bind63
Sider (fra-til)1-9
ISSN0043-1354
DOI
StatusUdgivet - okt. 2014

Emneord

  • Anaerobic digestion
  • Indigenous microorganisms
  • Resistance
  • Wastewater
  • Fish offal
  • Arctic

Citer dette

Gunnarsdottir, Ragnhildur ; Heiske, Stefan ; Jensen, Pernille Erland ; Schmidt, Jens Ejbye ; Villumsen, Arne ; Jenssen, Petter Deinboll. / Effect of anaerobiosis on indigenous microorganisms in blackwater with fish offal as co-substrate. I: Water Research. 2014 ; Bind 63. s. 1-9.
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abstract = "The aim of this study was to compare the effect of mesophilic anaerobic digestion with aerobic storage on the survival of selected indigenous microorganisms and microbial groups in blackwater, including the effect of addition of Greenlandic Halibut and shrimp offal. The methane yield of the different substrate mixtures was determined in batch experiments to study possible correlation between methanogenic activity in the anaerobic digesters and reduction of indigenous microorganisms in the blackwater. By the end of the experiments a recovery study was conducted to determine possible injury of the microorganisms. In both anaerobic and aerobic samples, survival of Escherichia coli was better in the presence of Greenlandic Halibut offal when compared to samples containing blackwater only and blackwater and shrimp offal, possibly due to more available carbon in the samples containing Greenlandic Halibut offal. Reduction of faecal streptococci was large under both anaerobic and aerobic conditions, and the results indicated a complete removal of faecal streptococci in the anaerobic samples containing blackwater and a mixture of blackwater and shrimp offal after 17 and 31 days, respectively. Amoxicillin resistant bacteria were reduced in the anaerobic samples in the beginning of the study but increased towards the end of it. The opposite pattern was observed in the aerobic samples, with a growth in the beginning followed by a reduction. During the anaerobic digestion tetracycline resistant bacteria showed the least reduction in the mixture of blackwater and shrimp offal, which had the lowest methane yield while the highest reduction was observed in the mixture of blackwater and Greenlandic Halibut, where the highest methane yield was measured Reduction of coliphages was larger under anaerobic conditions. Addition of fish offal had no effect on survival of coliphages. The results of the recovery study indicated that a fraction of the E. coli in the aerobic blackwater sample and of the faecal streptococci in both the anaerobic and aerobic samples containing blackwater and Greenlandic Halibut were injured only, and thus able to resuscitate during recovery.",
keywords = "Anaerobic digestion, Indigenous microorganisms, Resistance, Wastewater, Fish offal, Arctic",
author = "Ragnhildur Gunnarsdottir and Stefan Heiske and Jensen, {Pernille Erland} and Schmidt, {Jens Ejbye} and Arne Villumsen and Jenssen, {Petter Deinboll}",
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Effect of anaerobiosis on indigenous microorganisms in blackwater with fish offal as co-substrate. / Gunnarsdottir, Ragnhildur; Heiske, Stefan; Jensen, Pernille Erland; Schmidt, Jens Ejbye; Villumsen, Arne; Jenssen, Petter Deinboll.

I: Water Research, Bind 63, 10.2014, s. 1-9.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

TY - JOUR

T1 - Effect of anaerobiosis on indigenous microorganisms in blackwater with fish offal as co-substrate

AU - Gunnarsdottir, Ragnhildur

AU - Heiske, Stefan

AU - Jensen, Pernille Erland

AU - Schmidt, Jens Ejbye

AU - Villumsen, Arne

AU - Jenssen, Petter Deinboll

PY - 2014/10

Y1 - 2014/10

N2 - The aim of this study was to compare the effect of mesophilic anaerobic digestion with aerobic storage on the survival of selected indigenous microorganisms and microbial groups in blackwater, including the effect of addition of Greenlandic Halibut and shrimp offal. The methane yield of the different substrate mixtures was determined in batch experiments to study possible correlation between methanogenic activity in the anaerobic digesters and reduction of indigenous microorganisms in the blackwater. By the end of the experiments a recovery study was conducted to determine possible injury of the microorganisms. In both anaerobic and aerobic samples, survival of Escherichia coli was better in the presence of Greenlandic Halibut offal when compared to samples containing blackwater only and blackwater and shrimp offal, possibly due to more available carbon in the samples containing Greenlandic Halibut offal. Reduction of faecal streptococci was large under both anaerobic and aerobic conditions, and the results indicated a complete removal of faecal streptococci in the anaerobic samples containing blackwater and a mixture of blackwater and shrimp offal after 17 and 31 days, respectively. Amoxicillin resistant bacteria were reduced in the anaerobic samples in the beginning of the study but increased towards the end of it. The opposite pattern was observed in the aerobic samples, with a growth in the beginning followed by a reduction. During the anaerobic digestion tetracycline resistant bacteria showed the least reduction in the mixture of blackwater and shrimp offal, which had the lowest methane yield while the highest reduction was observed in the mixture of blackwater and Greenlandic Halibut, where the highest methane yield was measured Reduction of coliphages was larger under anaerobic conditions. Addition of fish offal had no effect on survival of coliphages. The results of the recovery study indicated that a fraction of the E. coli in the aerobic blackwater sample and of the faecal streptococci in both the anaerobic and aerobic samples containing blackwater and Greenlandic Halibut were injured only, and thus able to resuscitate during recovery.

AB - The aim of this study was to compare the effect of mesophilic anaerobic digestion with aerobic storage on the survival of selected indigenous microorganisms and microbial groups in blackwater, including the effect of addition of Greenlandic Halibut and shrimp offal. The methane yield of the different substrate mixtures was determined in batch experiments to study possible correlation between methanogenic activity in the anaerobic digesters and reduction of indigenous microorganisms in the blackwater. By the end of the experiments a recovery study was conducted to determine possible injury of the microorganisms. In both anaerobic and aerobic samples, survival of Escherichia coli was better in the presence of Greenlandic Halibut offal when compared to samples containing blackwater only and blackwater and shrimp offal, possibly due to more available carbon in the samples containing Greenlandic Halibut offal. Reduction of faecal streptococci was large under both anaerobic and aerobic conditions, and the results indicated a complete removal of faecal streptococci in the anaerobic samples containing blackwater and a mixture of blackwater and shrimp offal after 17 and 31 days, respectively. Amoxicillin resistant bacteria were reduced in the anaerobic samples in the beginning of the study but increased towards the end of it. The opposite pattern was observed in the aerobic samples, with a growth in the beginning followed by a reduction. During the anaerobic digestion tetracycline resistant bacteria showed the least reduction in the mixture of blackwater and shrimp offal, which had the lowest methane yield while the highest reduction was observed in the mixture of blackwater and Greenlandic Halibut, where the highest methane yield was measured Reduction of coliphages was larger under anaerobic conditions. Addition of fish offal had no effect on survival of coliphages. The results of the recovery study indicated that a fraction of the E. coli in the aerobic blackwater sample and of the faecal streptococci in both the anaerobic and aerobic samples containing blackwater and Greenlandic Halibut were injured only, and thus able to resuscitate during recovery.

KW - Anaerobic digestion

KW - Indigenous microorganisms

KW - Resistance

KW - Wastewater

KW - Fish offal

KW - Arctic

U2 - 10.1016/j.watres.2014.05.038

DO - 10.1016/j.watres.2014.05.038

M3 - Journal article

VL - 63

SP - 1

EP - 9

JO - Water Research

JF - Water Research

SN - 0043-1354

ER -