Earthworm tolerance to residual agricultural pesticide contamination: Field and experimental assessment of detoxification capabilities

Nicolas Givaudan, Françoise Binet, Barbara Le Bot, Claudia Wiegand*

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This study investigates if acclimatization to residual pesticide contamination in agricultural soils is reflected in detoxification, antioxidant enzyme activities and energy budget of earthworms. Five fields within a joint agricultural area exhibited different chemical and farming histories from conventional cultivation to organic pasture. Soil multiresidual pesticide analysis revealed up to 9 molecules including atrazine up to 2.4 ng g -1 dry soil. Exposure history of endogeic Aporrectodea caliginosa and Allolobophora chlorotica modified their responses to pesticides. In the field, activities of soluble glutathione-S-transferases (sGST) and catalase increased with soil pesticide contamination in A. caliginosa. Pesticide stress was reflected in depletion of energy reserves in A. chlorotica. Acute exposure of pre-adapted and naïve A. caliginosa to pesticides (fungicide Opus ®, 0.1 μg active ingredient epoxiconazole g-1 dry soil, RoundUp Flash®, 2.5 μg active ingredient glyphosate g-1 dry soil, and their mixture), revealed that environmental pre-exposure accelerated activation of the detoxification enzyme sGST towards epoxiconazole.

TidsskriftEnvironmental Pollution
Udgave nummerSeptember
Sider (fra-til)9-18
Antal sider10
StatusUdgivet - 2014